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cDNA Synthesis:

Article Title: Protumor effect of CXCL10/CXCR3 axis in canine mammary gland tumor.
Article Snippet: 2/12 https://doi.org/10.4142/jvs.24278https://vetsci.org Quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) Total RNA was isolated from CIPp and CIPm cells using TRIzol reagent (Ambion, USA) and quantified using a microplate spectrophotometer (Epoch; BioTek, USA). .. Total RNA was subjected to cDNA synthesis using a QuntiTect Reverse Transcription Kit (Enzynomics, Korea). .. An SYBR Green RT-PCR Kit (Enzynomics) was used for gene expression analysis.

Reverse Transcription:

Article Title: Protumor effect of CXCL10/CXCR3 axis in canine mammary gland tumor.
Article Snippet: 2/12 https://doi.org/10.4142/jvs.24278https://vetsci.org Quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) Total RNA was isolated from CIPp and CIPm cells using TRIzol reagent (Ambion, USA) and quantified using a microplate spectrophotometer (Epoch; BioTek, USA). .. Total RNA was subjected to cDNA synthesis using a QuntiTect Reverse Transcription Kit (Enzynomics, Korea). .. An SYBR Green RT-PCR Kit (Enzynomics) was used for gene expression analysis.



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