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Synthesized:Article Title: Engineering Anaerobic Metabolic Branch Points to Control Carbon Allocation Between Wax Ester Synthesis and Succinate Accumulation in Euglena gracilis Article Snippet: .. Complementary DNA was synthesized using Reverse Transcription:Article Title: Ac NR1F orchestrates epidermal remodeling to enable host adaptation in Angiostrongylus cantonensis Article Snippet: .. Total RNA of A. cantonensis was extracted using Trizol (Invitrogen, USA) and reverse transcribed to cDNA using Article Title: Unexpected roles of aromatic ring-cleaving dioxygenases in oxygen-heterocycle degradation: Functional diversity, gene redundancy, and ecological implications. Article Snippet: Oxygen-containing heterocycles (O-heterocycles), including tetrahydrofuran (THF) and 1,4-dioxane, are emerging water contaminants of growing concern because of their persistence and recalcitrance to conventional treatment processes.. Biodegradation of these compounds requires ring cleavage, yet the enzymes responsible for this critical step have remained unidentified.. By integrating affinity predictions with in vivo validation, we demonstrated that classical aromatic ring-cleaving dioxygenases, including the intradiol dioxygenases CatA1/ CatA2 from Cupriavidus metallidurans ZM02 and the extradiol dioxygenase MhpB from Escherichia coli BL21, mediate the cellular cleavage of 2-hydroxytetrahydrofuran (2-OH THF), a key intermediate in THF metabolism, to yield 4-hydroxybutyric acid (GHB). Article Title: PagCOBL5 Integrates Transcriptional and Spatial Cues to Confer G-Layer Deposition in Poplar. Article Snippet: Angiosperm trees reorient their stems by producing tension wood (TW), a specialised xylem that generates contractile force.. TW fibres develop a gelatinous (G) layer rich in cellulose, yet the molecular mechanisms governing G‐layer formation remain poorly understood.. COBRA‐like genes encode glycosylphosphatidylinositol (GPI)‐anchored proteins known to regulate cellulose deposition. Article Title: Pathogenicity and Genomic Features of a DAdV‐3 Strain Causing Severe Hepatitis in Muscovy Ducklings Article Snippet: The purity of isolated RNA samples was determined using a NanoDrop 2000 spectrophotometer. .. Qualified RNA samples were reverse‐transcribed into cDNA using the cDNA Synthesis:Article Title: Structural variation in the IAMH2 3′ UTR underlies hypocotyl elongation during light adaptation in Arabidopsis thaliana Article Snippet: Total RNA was extracted from manually dissected hypocotyls of 9‐day‐old seedlings using TRIzol reagent (Bio Fit, USA). .. cDNA synthesis was performed from 1 μg of RNA using the Centrifugation:Article Title: Unexpected roles of aromatic ring-cleaving dioxygenases in oxygen-heterocycle degradation: Functional diversity, gene redundancy, and ecological implications. Article Snippet: Oxygen-containing heterocycles (O-heterocycles), including tetrahydrofuran (THF) and 1,4-dioxane, are emerging water contaminants of growing concern because of their persistence and recalcitrance to conventional treatment processes.. Biodegradation of these compounds requires ring cleavage, yet the enzymes responsible for this critical step have remained unidentified.. By integrating affinity predictions with in vivo validation, we demonstrated that classical aromatic ring-cleaving dioxygenases, including the intradiol dioxygenases CatA1/ CatA2 from Cupriavidus metallidurans ZM02 and the extradiol dioxygenase MhpB from Escherichia coli BL21, mediate the cellular cleavage of 2-hydroxytetrahydrofuran (2-OH THF), a key intermediate in THF metabolism, to yield 4-hydroxybutyric acid (GHB). 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By integrating affinity predictions with in vivo validation, we demonstrated that classical aromatic ring-cleaving dioxygenases, including the intradiol dioxygenases CatA1/ CatA2 from Cupriavidus metallidurans ZM02 and the extradiol dioxygenase MhpB from Escherichia coli BL21, mediate the cellular cleavage of 2-hydroxytetrahydrofuran (2-OH THF), a key intermediate in THF metabolism, to yield 4-hydroxybutyric acid (GHB). Article Title: PagCOBL5 Integrates Transcriptional and Spatial Cues to Confer G-Layer Deposition in Poplar. Article Snippet: Angiosperm trees reorient their stems by producing tension wood (TW), a specialised xylem that generates contractile force.. TW fibres develop a gelatinous (G) layer rich in cellulose, yet the molecular mechanisms governing G‐layer formation remain poorly understood.. COBRA‐like genes encode glycosylphosphatidylinositol (GPI)‐anchored proteins known to regulate cellulose deposition. 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