Reverse Transcription:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Quantitative RT-PCR:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
SYBR Green Assay:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Concentration Assay:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Polymerase Chain Reaction:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Spectrophotometry:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Real-time Polymerase Chain Reaction:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Luminescence Assay:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Construct:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Membrane:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Software:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Expressing:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Amplification:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Gene Expression:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Functional Assay:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
cDNA Synthesis:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Fluorescence Microscopy:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Fluorescence:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Isolation:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Microscopy:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Incubation:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Clinical Proteomics:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
Bicinchoninic Acid Protein Assay:Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
Article Snippet: Izol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× S
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