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reverse transcriptase m mlv  (TaKaRa)


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    Structured Review

    TaKaRa reverse transcriptase m mlv
    Reverse Transcriptase M Mlv, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11036 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript%E2%84%A2+reverse+transcriptase/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc13077431-59-8-14
    Average 99 stars, based on 11036 article reviews
    reverse transcriptase m mlv - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Valorization of the oleaginous fungus Mortierella alpina for sustainable aquafeed development: Physiological and microbiome responses in zebrafish (Danio rerio)
    Article Snippet: Microbial bioresources are emerging as sustainable alternatives to traditional aquaculture feed ingredients.. While microbial oils derived from oleaginous microorganisms have demonstrated beneficial effects in aquaculture nutrition, the functional role of whole-cell biomass remains insufficiently explored.. Therefore, in this study, dried biomass of the oleaginous fungus Mortierella alpina EI-9 was assessed for safety and physiological effects in juvenile and adult zebrafish (Danio rerio) over a 60-day feeding trial.

    Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells.
    Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS.

    Article Title: Harnessing plant-derived bioactive compounds as LOX-1 inhibitors in Helicobacter pylori-driven gastric inflammation.
    Article Snippet: Helicobacter pylori persistence and rising antibiotic resistance have intensified interest in host-directed adjunctive strategies.. Recent studies identify LOX-1 as a gastric epithelial receptor for H. pylori catalase and support its role in bacterial adhesion, making it a plausible host-directed target.. In the current study, BI-0115, a structurally defined selective LOX-1 inhibitor, was used for functional validation in gastric epithelial cells infected with two clinical H. pylori isolates.

    Article Title: Quercetin Antagonizes Doxorubicin-Induced Cardiotoxicity via HO-1/PGC-1α-ALOX5 Axis: Arachidonic Acid Metabolism-Ferroptosis Crosstalk as a Therapeutic Target.
    Article Snippet: 4 School of Medical Technology, Gannan Medical University, Ganzhou 341000, China Abstract Myocardial injury caused by Doxorubicin has limited its clinical application.. Our research has demonstrated that doxorubicin induces various forms of cell death, oxidative stress, and metabolic abnormalities.. We have confirmed, through both in vitro and in vivo studies, that apoptosis, iron overload, inflammatory responses, and arachidonic acid metabolism contribute to doxorubicin’s cardiotoxic effects.

    Article Title: Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla
    Article Snippet: The GFP and DAPI fluorescence signals were separately visualized under a laser scanning confocal microscope (Zeiss LSM900, Germany). .. Total RNA was extracted from leaf, stem, and flower tissues of P. polyphylla using TaKaRa MiniBEST Plant RNA Extraction Kit (Takara, China), and the first-strand cDNA was synthesized through PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, China). .. For the PpUGTs expression analysis, qRT-PCR was performed on a qTOWER 3 G Real-Time PCR Thermal Cycler (Analytik Jena AG, Germany) using the PerfectStart® Green qPCR SuperMix (TransGen Biotech, China) according to the product manual.

    Article Title: Exon skipping by peptide nucleic acid derivatives
    Article Snippet: .. 200 ng of RNA template was subjected to a 25 μl reverse transcription reaction using PrimeScript 1st strand cDNA synthesis Kit (Cat. No. 6110B, Takara) against random hexamers. ..

    Article Title: Intracellular acid-base regulation mediates a trade-off between shell and somatic growth in a clam under ocean acidification
    Article Snippet: .. PrimeScript 1st strand cDNA synthesis kit , TaKaRa Biomedical Technology (Beijing) Co., Ltd. , 6110A. .. SMARTer® RACE 5’/3′ kit , Clontech , 634860.

    Article Title: Cathepsin A deficiency exacerbates LPS-induced inflammatory liver injury and apoptosis
    Article Snippet: Total RNA was isolated from spleen and liver tissue using the QIAzol lysis reagent (QIAGEN). .. RNA was reverse transcribed into cDNA using the PrimeScript 1st strand cDNA synthesis kit (TAKARA). .. Quantitative real-time PCR was performed using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (TAKARA) on a LightCycler 96 system.

    Reverse Transcription:

    Article Title: Valorization of the oleaginous fungus Mortierella alpina for sustainable aquafeed development: Physiological and microbiome responses in zebrafish (Danio rerio)
    Article Snippet: Microbial bioresources are emerging as sustainable alternatives to traditional aquaculture feed ingredients.. While microbial oils derived from oleaginous microorganisms have demonstrated beneficial effects in aquaculture nutrition, the functional role of whole-cell biomass remains insufficiently explored.. Therefore, in this study, dried biomass of the oleaginous fungus Mortierella alpina EI-9 was assessed for safety and physiological effects in juvenile and adult zebrafish (Danio rerio) over a 60-day feeding trial.

    Article Title: Quercetin Antagonizes Doxorubicin-Induced Cardiotoxicity via HO-1/PGC-1α-ALOX5 Axis: Arachidonic Acid Metabolism-Ferroptosis Crosstalk as a Therapeutic Target.
    Article Snippet: 4 School of Medical Technology, Gannan Medical University, Ganzhou 341000, China Abstract Myocardial injury caused by Doxorubicin has limited its clinical application.. Our research has demonstrated that doxorubicin induces various forms of cell death, oxidative stress, and metabolic abnormalities.. We have confirmed, through both in vitro and in vivo studies, that apoptosis, iron overload, inflammatory responses, and arachidonic acid metabolism contribute to doxorubicin’s cardiotoxic effects.

    Article Title: Exon skipping by peptide nucleic acid derivatives
    Article Snippet: .. 200 ng of RNA template was subjected to a 25 μl reverse transcription reaction using PrimeScript 1st strand cDNA synthesis Kit (Cat. No. 6110B, Takara) against random hexamers. ..

    Article Title: Cathepsin A deficiency exacerbates LPS-induced inflammatory liver injury and apoptosis
    Article Snippet: Total RNA was isolated from spleen and liver tissue using the QIAzol lysis reagent (QIAGEN). .. RNA was reverse transcribed into cDNA using the PrimeScript 1st strand cDNA synthesis kit (TAKARA). .. Quantitative real-time PCR was performed using TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (TAKARA) on a LightCycler 96 system.

    SYBR Green Assay:

    Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells.
    Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS.

    Polymerase Chain Reaction:

    Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells.
    Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS.

    RNA Extraction:

    Article Title: Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla
    Article Snippet: The GFP and DAPI fluorescence signals were separately visualized under a laser scanning confocal microscope (Zeiss LSM900, Germany). .. Total RNA was extracted from leaf, stem, and flower tissues of P. polyphylla using TaKaRa MiniBEST Plant RNA Extraction Kit (Takara, China), and the first-strand cDNA was synthesized through PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, China). .. For the PpUGTs expression analysis, qRT-PCR was performed on a qTOWER 3 G Real-Time PCR Thermal Cycler (Analytik Jena AG, Germany) using the PerfectStart® Green qPCR SuperMix (TransGen Biotech, China) according to the product manual.

    Synthesized:

    Article Title: Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla
    Article Snippet: The GFP and DAPI fluorescence signals were separately visualized under a laser scanning confocal microscope (Zeiss LSM900, Germany). .. Total RNA was extracted from leaf, stem, and flower tissues of P. polyphylla using TaKaRa MiniBEST Plant RNA Extraction Kit (Takara, China), and the first-strand cDNA was synthesized through PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, China). .. For the PpUGTs expression analysis, qRT-PCR was performed on a qTOWER 3 G Real-Time PCR Thermal Cycler (Analytik Jena AG, Germany) using the PerfectStart® Green qPCR SuperMix (TransGen Biotech, China) according to the product manual.



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