primer3plus software (PHUSA Biochem Ltd)
90
Structured Review
PHUSA Biochem Ltd
primer3plus software
Primer3plus Software, supplied by PHUSA Biochem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer3plus+program/primer3plus+software/pm36578946-82-7-14
Average 90 stars, based on 1 article reviews
Primer3plus Software, supplied by PHUSA Biochem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer3plus+program/primer3plus+software/pm36578946-82-7-14
Average 90 stars, based on 1 article reviews
primer3plus software - by Bioz Stars,
2026-09
90/100 stars
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Mutagenesis:Article Title: Analytical validation and clinical utilization of K-4CARE TM : a comprehensive genomic profiling assay with personalized MRD detection Article Snippet: .. The minimum amount of cfDNA required was ≥ 0.15 ng/uL or total of ≥ 3 ng. cfDNA fragments with the selected mutation sites were amplified using KAPA HiFi DNA Polymerase (Roche, USA) and targeted Amplification:Article Title: Analytical validation and clinical utilization of K-4CARE TM : a comprehensive genomic profiling assay with personalized MRD detection Article Snippet: .. The minimum amount of cfDNA required was ≥ 0.15 ng/uL or total of ≥ 3 ng. cfDNA fragments with the selected mutation sites were amplified using KAPA HiFi DNA Polymerase (Roche, USA) and targeted other:Article Title: Improved expression and purification of highly-active 3 chymotrypsin-like protease from SARS-CoV-2. Article Snippet: Severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2) is the causative pathogen of coronavirus disease19 (COVID-19).. The COVID-19 pandemic has resulted in millions of deaths and widespread socio-economic damage worldwide.. Therefore, numerous studies have been conducted to identify effective measures to control the spreading of the virus. Polymerase Chain Reaction:Article Title: Development of a Recombinase Polymerase Amplification Assay on Digital Microfluidics for Point-of-Care Detection of Plasmodium falciparum Article Snippet: 24 Malaria is a dangerous mosquito-borne infectious disease that causes millions of death cases each year 25 worldwide, especially in Southeast Asia.. Current P. falciparum detection methods (e.g., blood smear, 26 ELISA, PCR...) have many disadvantages, such as being time-consuming, dependent on the operator’s 27 experience, and relying on sophisticated instruments that hinder the point-of-care application.. To overcome 28 those drawbacks, we developed a colorimetric Recombinase Polymerase Amplification (RPA) reaction on 29 a Digital Microfluidic (DMF) platform using SYBR Green I as the indicator for a simple result readout 30 step, thus simplifying the diagnostic procedure. Article Title: KTt-45, a T-type calcium channel blocker, acts as an anticancer agent by inducing apoptosis on HeLa cervical cancer cell line Article Snippet: FITC Annexin-V Apoptosis Detection Kit I (#556547) was obtained from BD Biosciences (USA). .. TriSure, Tetro cDNA Synthesis Kit, and SensiFASTTM HRM Realtime PCR Kit were supplied by Bioline (UK). cDNA Synthesis:Article Title: KTt-45, a T-type calcium channel blocker, acts as an anticancer agent by inducing apoptosis on HeLa cervical cancer cell line Article Snippet: FITC Annexin-V Apoptosis Detection Kit I (#556547) was obtained from BD Biosciences (USA). .. TriSure, Tetro cDNA Synthesis Kit, and SensiFASTTM HRM Realtime PCR Kit were supplied by Bioline (UK). Real-time Polymerase Chain Reaction:Article Title: KTt-45, a T-type calcium channel blocker, acts as an anticancer agent by inducing apoptosis on HeLa cervical cancer cell line Article Snippet: FITC Annexin-V Apoptosis Detection Kit I (#556547) was obtained from BD Biosciences (USA). .. TriSure, Tetro cDNA Synthesis Kit, and SensiFASTTM HRM Realtime PCR Kit were supplied by Bioline (UK). Software:Article Title: Combination of Hotspot Mutations With Methylation and Fragmentomic Profiles to Enhance Multi‐Cancer Early Detection Article Snippet: Hotspot mutations were selected based on the following criteria: [ ] reported hotspot mutations in the COSMIC database that are recurrent in the five cancer types, [ ] reported actionable mutations, and [ ] mutations found in tumor tissue from the Vietnamese cancer patients according to five types of cancers (breast, colorectal, gastric, liver, and lung cancer). .. Compatible primer pairs were designed using Article Title: Combination of hotspot mutations with methylation and fragmentomic profiles to enhance Multi-Cancer Early Detection Article Snippet: Hotspot mutations were selected based on the following criteria: (1) reported hotspot mutations in the COSMIC database that are recurrent in the five cancer types, (2) reported actionable mutations, and (3) mutations found in tumor tissue from the Vietnamese cancer patients according to five types of cancers (breast, colorectal, gastric, liver and lung cancer). .. Compatible primer pairs were designed using Synthesized:Article Title: Combination of Hotspot Mutations With Methylation and Fragmentomic Profiles to Enhance Multi‐Cancer Early Detection Article Snippet: Hotspot mutations were selected based on the following criteria: [ ] reported hotspot mutations in the COSMIC database that are recurrent in the five cancer types, [ ] reported actionable mutations, and [ ] mutations found in tumor tissue from the Vietnamese cancer patients according to five types of cancers (breast, colorectal, gastric, liver, and lung cancer). .. Compatible primer pairs were designed using Article Title: CRISPR/Cas9 targeted mutations of OsDSG1 gene enhanced salt tolerance in rice. Article Snippet: Salinization is one of the leading causes of arable land shrinkage and rice yield decline, recently.. Therefore, developing and utilizing salt-tolerant rice varieties have been seen as a crucial and urgent strategy to reduce the effects of saline intrusion and protect food security worldwide.. In the current study, the CRISPR/Cas9 system was utilized to induce targeted mutations in the coding sequence of the OsDSG1, a gene involved in the ubiquitination pathway and the regulation of biochemical reactions in rice. Article Title: Combination of hotspot mutations with methylation and fragmentomic profiles to enhance Multi-Cancer Early Detection Article Snippet: Hotspot mutations were selected based on the following criteria: (1) reported hotspot mutations in the COSMIC database that are recurrent in the five cancer types, (2) reported actionable mutations, and (3) mutations found in tumor tissue from the Vietnamese cancer patients according to five types of cancers (breast, colorectal, gastric, liver and lung cancer). .. Compatible primer pairs were designed using |