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Premier Biosoft computer program primer premier 5.0
Computer Program Primer Premier 5.0, supplied by Premier Biosoft, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+premier+computer+program/computer+software+primer+premier+5+0/pmc02845116-57-15-20
Average 90 stars, based on 1 article reviews
computer program primer premier 5.0 - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Identification of pathogenicity, investigation of virulent gene distribution and development of a virulent strain-specific detection PCR method for Vibrio harveyi isolated from Hainan Province and Guangdong Province, China
Article Snippet: Article history: Received 9 July 2016 Received in revised form 3 October 2016 Accepted 7 October 2016 Available online 11 October 2016 A collection of 46 Vibrio harveyi strains were isolated from Epinephelus spp., Lutjanus erythopterus, and other maricultured fish in coastal areas of Hainan Province and Guangdong Province, China, between 2011 and 2013.. Eighteen strains were determined to be pathogenic via artificial infection of healthy Epinephelus coioides at 10 colony-forming units (CFU) mL.. Mortality occurred within 2 to 6 h after injection.

Article Title: Seasonal differences in the transcriptome profile of the Zhedong white goose ( Anser cygnoides ) pituitary gland
Article Snippet: The qRT-PCR primers were designed using Primer Premier 5.0 software ( www.PremierBiosoft.com ) and synthesized by Generay biotechnology (Shanghai, China).

Article Title: Nitric Oxide Plays a Central Role in Water Stress-Induced Tanshinone Production in Salvia miltiorrhiza Hairy Roots
Article Snippet: The primers were designed by the software Primer-Premier 5.0 (Premier Biosoft International, Palo Alto, CA, USA) ( ).

Article Title: IGFBP7 promotes the proliferation and differentiation of primary myoblasts and intramuscular preadipocytes in chicken
Article Snippet: The qPCR primers ( ) were designed using Primer Premier 5.0 software (Premier Biosoft International, Palo Alto, CA) and synthesized by TsingkeBiotechnology (Zhengzhou, China).

Article Title: Heat-shock protein 70 reduces apoptosis in the gills and hepatopancreas of Marsupenaeus japonicus under low-temperature stress.
Article Snippet: The heat shock response (HSR) has important functions in a cell's reaction to stresses.. The HSR is regulated and effected by heat shock proteins (HSPs) and heat shock factors (HSFs).. Herein, the full-length cDNA of Marsupenaeus japonicus heat shock protein 70 (MjHSP70) was isolated and sequenced.

Article Title: Identification of IκBα in Japanese eel Anguilla japonica that impairs the IKKα-dependent activation of NF-κB, AP1, and type I IFN signaling pathways.
Article Snippet: As a member of inhibitory κB family (IκB) family, IκBα is best-characterized and plays a central negative feedback regulator of NF-κB pathway in mammals, but the information about IκBα in the regulation of immune responses is still limited in teleost fishes.. In the present study, the full-length cDNA of an IκBα homologue, AjIκBα, was cloned by 5 and 3 SMART RACE from Japanese eel, and its characteristics of expression in response to various PAMPs and A. hydrophila infection were investigated both in vivo and in vitro using quantitative realtime polymerase chain reaction (qRT-PCR).. In addition, the subcellular localization of AjIκBα GFP fusion protein and the induction of AjIκBα alone or co-expression with Japanese eel IKKα (AjIKKα) in the activation of NF-κB, type I IFN and AP1 performed using Dual-Glo luciferase assay system were also detected.

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Article Title: Molecular Cloning and Expression Analysis of Thyrotropin-Releasing Hormone, and Its Possible Role in Gonadal Differentiation in Rice Field eel Monopterus albus
Article Snippet: After transformation into Escherichia coli DH5α competent cells (Takara, Beijing, China), the recombinant plasmid was confirmed by DNA sequencing of positive clones. .. In this study, all the primers were designed using the classical software of Primer Premier 5.0 (Premier Biosoft International, Palo Alto, CA, USA) ( ). .. Both the synthesis of primers and sequencing of positive products were performed by Sangon Biotech Company in Shanghai, China.

Article Title: Molecular Cloning and Expression Analysis of Thyrotropin-Releasing Hormone, and Its Possible Role in Gonadal Differentiation in Rice Field eel Monopterus albus .
Article Snippet: After transformation into Escherichia coli DH5α competent cells (Takara, Beijing, China), the recombinant plasmid was confirmed by DNA sequencing of positive clones. .. In this study, all the primers were designed using the classical software of Primer Premier 5.0 (Premier Biosoft International, Palo Alto, CA, USA) (Table 1). .. Both the synthesis of primers and sequencing of positive products were performed by Sangon Biotech Company in Shanghai, China.



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