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Schuelke tailed primer method
Tailed Primer Method, supplied by Schuelke, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/tailed+primer+method/pmc11785656-117-7-10
Average 90 stars, based on 1 article reviews
tailed primer method - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Amplification:

Article Title: Assessment of the Origin and Diversity of Croatian Common Bean Germplasm Using Phaseolin Type, SSR and SNP Markers and Morphological Traits
Article Snippet: .. Amplification of phaseolin sequences [ ] was used to determine the phaseolin type of each accession using the tailed PCR approach developed by Schuelke [ ]. ..

Article Title: Identifying Priority Giant Anteater ( Myrmecophaga tridactyla ) Populations for Conservation in São Paulo State, Brazil
Article Snippet: .. Amplification via PCR was performed with the addition of the M13 tail, which allowed for the use of the tailed primer method (Schuelke, ). .. The amplifications were performed using touchdown PCR in a total volume of 17.2 μl, which contained 1 μl of DNA (~50 ηg), 0.2 μl of the forward primer (5 μM), 0.4 μl of the M13 primer dyed with fluorophore (5 μ), 0.6 μl of the reverse primer (5 μM), 5 μl of the Gotaq ® Colorless Master Mix (Promega; 2X), and 10 μl of ultrapure water (Don et al., ; Korbie & Mattick, ).

Article Title: Genome-Assisted Gene-Flow Rescued Genetic Diversity Without Hindering Growth Performance in an Inbred Coho Salmon ( Oncorhynchus kisutch ) Population Selected for High Growth Phenotype
Article Snippet: Genomic DNA was collected from an adipose fin clip using a Gentra Puregene Tissue Kit (QIAGEN). .. The 11 loci were amplified using the tailed primer method (Schuelke ; Sekino et al. ) where 19-bp of the M13 sequence (CACGACGTTGTAAAACGAC) was added to the 5' end of the forward primer to label PCR amplicon with a fluorescent dye (i.e., FAM, VIC, NED, PED). .. Polymorphisms were analyzed on an ABI3130 DNA Analyzer (Applied Biosystems, MA, USA).

Polymerase Chain Reaction:

Article Title: Assessment of the Origin and Diversity of Croatian Common Bean Germplasm Using Phaseolin Type, SSR and SNP Markers and Morphological Traits
Article Snippet: .. Amplification of phaseolin sequences [ ] was used to determine the phaseolin type of each accession using the tailed PCR approach developed by Schuelke [ ]. ..

Article Title: Identifying Priority Giant Anteater ( Myrmecophaga tridactyla ) Populations for Conservation in São Paulo State, Brazil
Article Snippet: .. Amplification via PCR was performed with the addition of the M13 tail, which allowed for the use of the tailed primer method (Schuelke, ). .. The amplifications were performed using touchdown PCR in a total volume of 17.2 μl, which contained 1 μl of DNA (~50 ηg), 0.2 μl of the forward primer (5 μM), 0.4 μl of the M13 primer dyed with fluorophore (5 μ), 0.6 μl of the reverse primer (5 μM), 5 μl of the Gotaq ® Colorless Master Mix (Promega; 2X), and 10 μl of ultrapure water (Don et al., ; Korbie & Mattick, ).

Article Title: Metapopulation of Ellobium Chinense Through The Late-Middle and Late Pleistocene Expansions: Four Covariate COI Hotspots Linked to G-Quadruplex Conformation
Article Snippet: .. A tailed PCR primer was produced by adding a M13 (-21) universal sequence “tag” to the 5′ end of each forward primer as described by Schuelke (2000)37. ..

Article Title: Characterization of metapopulation of Ellobium chinense through Pleistocene expansions and four covariate COI guanine-hotspots linked to G-quadruplex conformation
Article Snippet: .. A tailed PCR primer was produced by adding a M13 (− 21) universal sequence “tag” to the 5′ end of each forward primer as described by Schuelke . ..

Article Title: Genome-Assisted Gene-Flow Rescued Genetic Diversity Without Hindering Growth Performance in an Inbred Coho Salmon ( Oncorhynchus kisutch ) Population Selected for High Growth Phenotype
Article Snippet: Genomic DNA was collected from an adipose fin clip using a Gentra Puregene Tissue Kit (QIAGEN). .. The 11 loci were amplified using the tailed primer method (Schuelke ; Sekino et al. ) where 19-bp of the M13 sequence (CACGACGTTGTAAAACGAC) was added to the 5' end of the forward primer to label PCR amplicon with a fluorescent dye (i.e., FAM, VIC, NED, PED). .. Polymorphisms were analyzed on an ABI3130 DNA Analyzer (Applied Biosystems, MA, USA).

Article Title: Quantitative trait loci conferring blast resistance in hexaploid wheat at adult plant stage
Article Snippet: Magnaporthe oryzae (anamorphic synonym Pyricularia oryzae) pathotype Triticum (MoT) is the causal agent of wheat blast.. The pathogen can infect all the aboveground tissues of wheat plants, where different types of lesions can be observed.. However, the most destructive symptom occurs in wheat spikes that become discolored (bleached).

Article Title: The first reproductive parameters and evidence of multiple paternity in one new spiny dogfish species, Squalus albicaudus (Squaliformes, Squalidae).
Article Snippet: Understanding elasmobranch reproductive biology is necessary for species conservation.. Multiple paternity has been reported for elasmobranchs and here we investigate the reproductive aspects and mating system (paternity genetic analyses) for Squalus albicaudus.. Thirteen pregnant females were analyzed concerning reproductive parameters and mating system was assessed of nine females and their litters.

Produced:

Article Title: Metapopulation of Ellobium Chinense Through The Late-Middle and Late Pleistocene Expansions: Four Covariate COI Hotspots Linked to G-Quadruplex Conformation
Article Snippet: .. A tailed PCR primer was produced by adding a M13 (-21) universal sequence “tag” to the 5′ end of each forward primer as described by Schuelke (2000)37. ..

Article Title: Characterization of metapopulation of Ellobium chinense through Pleistocene expansions and four covariate COI guanine-hotspots linked to G-quadruplex conformation
Article Snippet: .. A tailed PCR primer was produced by adding a M13 (− 21) universal sequence “tag” to the 5′ end of each forward primer as described by Schuelke . ..

Sequencing:

Article Title: Metapopulation of Ellobium Chinense Through The Late-Middle and Late Pleistocene Expansions: Four Covariate COI Hotspots Linked to G-Quadruplex Conformation
Article Snippet: .. A tailed PCR primer was produced by adding a M13 (-21) universal sequence “tag” to the 5′ end of each forward primer as described by Schuelke (2000)37. ..

Article Title: Characterization of metapopulation of Ellobium chinense through Pleistocene expansions and four covariate COI guanine-hotspots linked to G-quadruplex conformation
Article Snippet: .. A tailed PCR primer was produced by adding a M13 (− 21) universal sequence “tag” to the 5′ end of each forward primer as described by Schuelke . ..

Article Title: Genome-Assisted Gene-Flow Rescued Genetic Diversity Without Hindering Growth Performance in an Inbred Coho Salmon ( Oncorhynchus kisutch ) Population Selected for High Growth Phenotype
Article Snippet: Genomic DNA was collected from an adipose fin clip using a Gentra Puregene Tissue Kit (QIAGEN). .. The 11 loci were amplified using the tailed primer method (Schuelke ; Sekino et al. ) where 19-bp of the M13 sequence (CACGACGTTGTAAAACGAC) was added to the 5' end of the forward primer to label PCR amplicon with a fluorescent dye (i.e., FAM, VIC, NED, PED). .. Polymorphisms were analyzed on an ABI3130 DNA Analyzer (Applied Biosystems, MA, USA).

Article Title: Quantitative trait loci conferring blast resistance in hexaploid wheat at adult plant stage
Article Snippet: Magnaporthe oryzae (anamorphic synonym Pyricularia oryzae) pathotype Triticum (MoT) is the causal agent of wheat blast.. The pathogen can infect all the aboveground tissues of wheat plants, where different types of lesions can be observed.. However, the most destructive symptom occurs in wheat spikes that become discolored (bleached).

Marker:

Article Title: Quantitative trait loci conferring blast resistance in hexaploid wheat at adult plant stage
Article Snippet: Magnaporthe oryzae (anamorphic synonym Pyricularia oryzae) pathotype Triticum (MoT) is the causal agent of wheat blast.. The pathogen can infect all the aboveground tissues of wheat plants, where different types of lesions can be observed.. However, the most destructive symptom occurs in wheat spikes that become discolored (bleached).



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List of tested (white fields) and reference genes.
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Image Search Results


List of tested (white fields) and reference genes.

Journal: International Journal of Molecular Sciences

Article Title: Biosynthesis of Phenolic Compounds of Medicago truncatula After Inoculation with Selected PGPR Strains

doi: 10.3390/ijms252312684

Figure Lengend Snippet: List of tested (white fields) and reference genes.

Article Snippet: PCR primers for both semi-quantitative and quantitative methods were designed using Primer Express 3.0 software (Applied Biosystems, Carlsbad, CA, USA)— .

Techniques: Sequencing