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crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid  (Addgene inc)


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    Addgene inc crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid
    Crispr Cas9 Genomic Engineering Idt Star Methods Rbns Primers Idt Star Methods Recombinant Dna Px458 Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4076 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer+method/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm41932309-792-83-97
    Average 96 stars, based on 4076 article reviews
    crispr cas9 genomic engineering idt star methods rbns primers idt star methods recombinant dna px458 addgene plasmid - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Mass Spectrometry:

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Flag Sigma F3165; RRID: AB_259529 TOMM22 ThermoFisher PA5-51804; RRID AB_2648810 U2AF2 Santa Cruz sc-53942; RRID: AB_831787 EIF3 Bethyl A301-746A-M GIGYF2 Bethyl A303-732A G3BP1 Invitrogen PA5-29455; RRID: AB_2546931 RPS19 Bethyl A304-002A BETA ACTIN Sigma A2228; RRID: AB_476697 HSPD1 Sigma HPA050025; RRID: AB_2680995 Alexa Fluor 488-conjugated anti-Puromycin Biolegend 381506; RRID: AB_2927825 CD34 FITC BD 555821; RRID: AB_396150 CD11b PE Beckman Coulter IM2581U; RRID: AB_131334 GlycophorinA BV421 BD 562938; RRID: AB_2721016 Anti-Puromycin AlexaFluor647 PMY-2A4 Donated by Jonthan Yewdell RRID:AB_2619605 Anti-Puromycin 2A4 AlexaFluor647 Biolegend 381507; RRID: AB_2927826 Biological samples Human bone marrow mono-nuclear primary samples Tampa General Hospital–University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) and Lombardi Cancer Institute, School of Medicine, Georgetown University; Washington, D.C. N/A Chemicals, peptides, and recombinant proteins 3XFlag peptide Sigma F4799 Puromycin Sigma P4512-1MLX10 2-Deoxy-D-glucose (2DG) Sigma D8375-5G Cycloheximide Sigma 239764-1GM Oligomycin Sigma 04876 Critical commercial assays LIVE/DEADTM Fixable Near-IR: Dead Cell Stain Kit Thermo Fisher L10119 EasySepTM Human CD34 Positive Selection Kit II STEMCELL Technologies 17856 Mitochondria Isolation Kit for Cultured Cells Thermo Fisher 89874 EasyPep kit Thermo Fisher A40006 HiScribe T7 RNA synthesis kit NEB E2040S Monarch RNA cleanup kit NEB T2040L DNA Clean & Concentrator Kit Zymo D4003 HiScribe SP6 RNA synthesis kit NEB E2070S Nano-Glo Luciferase Assay Promega N1110 MitoTracker Red CMXRos Invitrogen 2351994 FoxP3 Staining Buffer Set Thermo Fisher 00-5523-00 ImProm-II reverse transcription system Promega A3800 (Continued on next page) Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Mass spec This study MassIVE: MSV000100675 Ribo-sequencing This study GEO: GSE318055 RBNS Donavanet al.49 N/A Experimental models: Cell lines HBEC3-KT U2AF1wt/wt cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/S34F cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/wt-3XFlag cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) This study N/A Oligonucleotides sgRNA and primers for Crispr/CAS9 genomic engineering IDT Star Methods RBNS primers IDT Star Methods Recombinant DNA PX458 Addgene Plasmid #48138 U2AF1 C-terminal 3XFlag Donor plasmid This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-ΔCDs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT:1-126bp This study Addgene Plasmid Nano luciferase in vitro reporter: 1xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: 2xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs:1–126bp This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_wt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_mt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid In vivo reporter: ATP5F1B-mcherry Addgene # 232783 In vivo reporter: ATP5F1B-mcherry-mtAG This study Addgene Plasmid Software and algorithms Gene Set Enrichment Analysis (GSEA) Subramanian et al.33 N/A Fiji, open-source image processing package github.com/fiji/fiji N/A Bowtie Langmead et al.73 N/A wavClusteR Comoglio et al.74 N/A Enrichr online resource Kuleshov et al.75 N/A deeptools Ramı́rez et al.76 N/A RBNS pipeline Lambert, Robertson et al.77 N/A Probound Rube et al.78 N/A FIMO algorithm from the meme suite Grant et al.43 N/A cutadapt Kechin et al.79 N/A Bowtie2 Langmead and Salzberg80 N/A CellProfiler Stirling et al.81 N/A FISH-quant v2 big-FISH package Imbert et al.82 N/A Mitochondria Analyzer Chaudhry et al.83 N/A empanada-napari Conrad and Narayan84 N/A Other Anti-Flag M2 magnetic beads Sigma M8823 e2 Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 .. Human Bronchial Epithelial Cells (HBECs) were cultured in Keratinocyte SFM media (Gibco, 17005042) in the presence of 5% CO2 at 37 oC in a humidified incubator.

    CRISPR:

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Flag Sigma F3165; RRID: AB_259529 TOMM22 ThermoFisher PA5-51804; RRID AB_2648810 U2AF2 Santa Cruz sc-53942; RRID: AB_831787 EIF3 Bethyl A301-746A-M GIGYF2 Bethyl A303-732A G3BP1 Invitrogen PA5-29455; RRID: AB_2546931 RPS19 Bethyl A304-002A BETA ACTIN Sigma A2228; RRID: AB_476697 HSPD1 Sigma HPA050025; RRID: AB_2680995 Alexa Fluor 488-conjugated anti-Puromycin Biolegend 381506; RRID: AB_2927825 CD34 FITC BD 555821; RRID: AB_396150 CD11b PE Beckman Coulter IM2581U; RRID: AB_131334 GlycophorinA BV421 BD 562938; RRID: AB_2721016 Anti-Puromycin AlexaFluor647 PMY-2A4 Donated by Jonthan Yewdell RRID:AB_2619605 Anti-Puromycin 2A4 AlexaFluor647 Biolegend 381507; RRID: AB_2927826 Biological samples Human bone marrow mono-nuclear primary samples Tampa General Hospital–University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) and Lombardi Cancer Institute, School of Medicine, Georgetown University; Washington, D.C. N/A Chemicals, peptides, and recombinant proteins 3XFlag peptide Sigma F4799 Puromycin Sigma P4512-1MLX10 2-Deoxy-D-glucose (2DG) Sigma D8375-5G Cycloheximide Sigma 239764-1GM Oligomycin Sigma 04876 Critical commercial assays LIVE/DEADTM Fixable Near-IR: Dead Cell Stain Kit Thermo Fisher L10119 EasySepTM Human CD34 Positive Selection Kit II STEMCELL Technologies 17856 Mitochondria Isolation Kit for Cultured Cells Thermo Fisher 89874 EasyPep kit Thermo Fisher A40006 HiScribe T7 RNA synthesis kit NEB E2040S Monarch RNA cleanup kit NEB T2040L DNA Clean & Concentrator Kit Zymo D4003 HiScribe SP6 RNA synthesis kit NEB E2070S Nano-Glo Luciferase Assay Promega N1110 MitoTracker Red CMXRos Invitrogen 2351994 FoxP3 Staining Buffer Set Thermo Fisher 00-5523-00 ImProm-II reverse transcription system Promega A3800 (Continued on next page) Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Mass spec This study MassIVE: MSV000100675 Ribo-sequencing This study GEO: GSE318055 RBNS Donavanet al.49 N/A Experimental models: Cell lines HBEC3-KT U2AF1wt/wt cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/S34F cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/wt-3XFlag cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) This study N/A Oligonucleotides sgRNA and primers for Crispr/CAS9 genomic engineering IDT Star Methods RBNS primers IDT Star Methods Recombinant DNA PX458 Addgene Plasmid #48138 U2AF1 C-terminal 3XFlag Donor plasmid This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-ΔCDs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT:1-126bp This study Addgene Plasmid Nano luciferase in vitro reporter: 1xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: 2xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs:1–126bp This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_wt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_mt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid In vivo reporter: ATP5F1B-mcherry Addgene # 232783 In vivo reporter: ATP5F1B-mcherry-mtAG This study Addgene Plasmid Software and algorithms Gene Set Enrichment Analysis (GSEA) Subramanian et al.33 N/A Fiji, open-source image processing package github.com/fiji/fiji N/A Bowtie Langmead et al.73 N/A wavClusteR Comoglio et al.74 N/A Enrichr online resource Kuleshov et al.75 N/A deeptools Ramı́rez et al.76 N/A RBNS pipeline Lambert, Robertson et al.77 N/A Probound Rube et al.78 N/A FIMO algorithm from the meme suite Grant et al.43 N/A cutadapt Kechin et al.79 N/A Bowtie2 Langmead and Salzberg80 N/A CellProfiler Stirling et al.81 N/A FISH-quant v2 big-FISH package Imbert et al.82 N/A Mitochondria Analyzer Chaudhry et al.83 N/A empanada-napari Conrad and Narayan84 N/A Other Anti-Flag M2 magnetic beads Sigma M8823 e2 Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 .. Human Bronchial Epithelial Cells (HBECs) were cultured in Keratinocyte SFM media (Gibco, 17005042) in the presence of 5% CO2 at 37 oC in a humidified incubator.

    Article Title: Matrix stiffness induces midnolin-dependent lamin B1 degradation to control myoblast differentiation.
    Article Snippet: Samples were run on a QuantStudio 3 Real-time PCR System (Applied Biosystems). .. CRISPR-mediated lamin B1 gene knockout To knockout lamin B1 genes, we first inserted mLmnb1 gRNA into pSpCas9(BB)-2A-GFP (PX458) (addgene#48138). ..

    Recombinant:

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Flag Sigma F3165; RRID: AB_259529 TOMM22 ThermoFisher PA5-51804; RRID AB_2648810 U2AF2 Santa Cruz sc-53942; RRID: AB_831787 EIF3 Bethyl A301-746A-M GIGYF2 Bethyl A303-732A G3BP1 Invitrogen PA5-29455; RRID: AB_2546931 RPS19 Bethyl A304-002A BETA ACTIN Sigma A2228; RRID: AB_476697 HSPD1 Sigma HPA050025; RRID: AB_2680995 Alexa Fluor 488-conjugated anti-Puromycin Biolegend 381506; RRID: AB_2927825 CD34 FITC BD 555821; RRID: AB_396150 CD11b PE Beckman Coulter IM2581U; RRID: AB_131334 GlycophorinA BV421 BD 562938; RRID: AB_2721016 Anti-Puromycin AlexaFluor647 PMY-2A4 Donated by Jonthan Yewdell RRID:AB_2619605 Anti-Puromycin 2A4 AlexaFluor647 Biolegend 381507; RRID: AB_2927826 Biological samples Human bone marrow mono-nuclear primary samples Tampa General Hospital–University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) and Lombardi Cancer Institute, School of Medicine, Georgetown University; Washington, D.C. N/A Chemicals, peptides, and recombinant proteins 3XFlag peptide Sigma F4799 Puromycin Sigma P4512-1MLX10 2-Deoxy-D-glucose (2DG) Sigma D8375-5G Cycloheximide Sigma 239764-1GM Oligomycin Sigma 04876 Critical commercial assays LIVE/DEADTM Fixable Near-IR: Dead Cell Stain Kit Thermo Fisher L10119 EasySepTM Human CD34 Positive Selection Kit II STEMCELL Technologies 17856 Mitochondria Isolation Kit for Cultured Cells Thermo Fisher 89874 EasyPep kit Thermo Fisher A40006 HiScribe T7 RNA synthesis kit NEB E2040S Monarch RNA cleanup kit NEB T2040L DNA Clean & Concentrator Kit Zymo D4003 HiScribe SP6 RNA synthesis kit NEB E2070S Nano-Glo Luciferase Assay Promega N1110 MitoTracker Red CMXRos Invitrogen 2351994 FoxP3 Staining Buffer Set Thermo Fisher 00-5523-00 ImProm-II reverse transcription system Promega A3800 (Continued on next page) Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Mass spec This study MassIVE: MSV000100675 Ribo-sequencing This study GEO: GSE318055 RBNS Donavanet al.49 N/A Experimental models: Cell lines HBEC3-KT U2AF1wt/wt cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/S34F cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/wt-3XFlag cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) This study N/A Oligonucleotides sgRNA and primers for Crispr/CAS9 genomic engineering IDT Star Methods RBNS primers IDT Star Methods Recombinant DNA PX458 Addgene Plasmid #48138 U2AF1 C-terminal 3XFlag Donor plasmid This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-ΔCDs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT:1-126bp This study Addgene Plasmid Nano luciferase in vitro reporter: 1xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: 2xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs:1–126bp This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_wt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_mt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid In vivo reporter: ATP5F1B-mcherry Addgene # 232783 In vivo reporter: ATP5F1B-mcherry-mtAG This study Addgene Plasmid Software and algorithms Gene Set Enrichment Analysis (GSEA) Subramanian et al.33 N/A Fiji, open-source image processing package github.com/fiji/fiji N/A Bowtie Langmead et al.73 N/A wavClusteR Comoglio et al.74 N/A Enrichr online resource Kuleshov et al.75 N/A deeptools Ramı́rez et al.76 N/A RBNS pipeline Lambert, Robertson et al.77 N/A Probound Rube et al.78 N/A FIMO algorithm from the meme suite Grant et al.43 N/A cutadapt Kechin et al.79 N/A Bowtie2 Langmead and Salzberg80 N/A CellProfiler Stirling et al.81 N/A FISH-quant v2 big-FISH package Imbert et al.82 N/A Mitochondria Analyzer Chaudhry et al.83 N/A empanada-napari Conrad and Narayan84 N/A Other Anti-Flag M2 magnetic beads Sigma M8823 e2 Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 .. Human Bronchial Epithelial Cells (HBECs) were cultured in Keratinocyte SFM media (Gibco, 17005042) in the presence of 5% CO2 at 37 oC in a humidified incubator.

    Plasmid Preparation:

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Flag Sigma F3165; RRID: AB_259529 TOMM22 ThermoFisher PA5-51804; RRID AB_2648810 U2AF2 Santa Cruz sc-53942; RRID: AB_831787 EIF3 Bethyl A301-746A-M GIGYF2 Bethyl A303-732A G3BP1 Invitrogen PA5-29455; RRID: AB_2546931 RPS19 Bethyl A304-002A BETA ACTIN Sigma A2228; RRID: AB_476697 HSPD1 Sigma HPA050025; RRID: AB_2680995 Alexa Fluor 488-conjugated anti-Puromycin Biolegend 381506; RRID: AB_2927825 CD34 FITC BD 555821; RRID: AB_396150 CD11b PE Beckman Coulter IM2581U; RRID: AB_131334 GlycophorinA BV421 BD 562938; RRID: AB_2721016 Anti-Puromycin AlexaFluor647 PMY-2A4 Donated by Jonthan Yewdell RRID:AB_2619605 Anti-Puromycin 2A4 AlexaFluor647 Biolegend 381507; RRID: AB_2927826 Biological samples Human bone marrow mono-nuclear primary samples Tampa General Hospital–University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) and Lombardi Cancer Institute, School of Medicine, Georgetown University; Washington, D.C. N/A Chemicals, peptides, and recombinant proteins 3XFlag peptide Sigma F4799 Puromycin Sigma P4512-1MLX10 2-Deoxy-D-glucose (2DG) Sigma D8375-5G Cycloheximide Sigma 239764-1GM Oligomycin Sigma 04876 Critical commercial assays LIVE/DEADTM Fixable Near-IR: Dead Cell Stain Kit Thermo Fisher L10119 EasySepTM Human CD34 Positive Selection Kit II STEMCELL Technologies 17856 Mitochondria Isolation Kit for Cultured Cells Thermo Fisher 89874 EasyPep kit Thermo Fisher A40006 HiScribe T7 RNA synthesis kit NEB E2040S Monarch RNA cleanup kit NEB T2040L DNA Clean & Concentrator Kit Zymo D4003 HiScribe SP6 RNA synthesis kit NEB E2070S Nano-Glo Luciferase Assay Promega N1110 MitoTracker Red CMXRos Invitrogen 2351994 FoxP3 Staining Buffer Set Thermo Fisher 00-5523-00 ImProm-II reverse transcription system Promega A3800 (Continued on next page) Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Mass spec This study MassIVE: MSV000100675 Ribo-sequencing This study GEO: GSE318055 RBNS Donavanet al.49 N/A Experimental models: Cell lines HBEC3-KT U2AF1wt/wt cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/S34F cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/wt-3XFlag cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) This study N/A Oligonucleotides sgRNA and primers for Crispr/CAS9 genomic engineering IDT Star Methods RBNS primers IDT Star Methods Recombinant DNA PX458 Addgene Plasmid #48138 U2AF1 C-terminal 3XFlag Donor plasmid This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-ΔCDs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT:1-126bp This study Addgene Plasmid Nano luciferase in vitro reporter: 1xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: 2xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs:1–126bp This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_wt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_mt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid In vivo reporter: ATP5F1B-mcherry Addgene # 232783 In vivo reporter: ATP5F1B-mcherry-mtAG This study Addgene Plasmid Software and algorithms Gene Set Enrichment Analysis (GSEA) Subramanian et al.33 N/A Fiji, open-source image processing package github.com/fiji/fiji N/A Bowtie Langmead et al.73 N/A wavClusteR Comoglio et al.74 N/A Enrichr online resource Kuleshov et al.75 N/A deeptools Ramı́rez et al.76 N/A RBNS pipeline Lambert, Robertson et al.77 N/A Probound Rube et al.78 N/A FIMO algorithm from the meme suite Grant et al.43 N/A cutadapt Kechin et al.79 N/A Bowtie2 Langmead and Salzberg80 N/A CellProfiler Stirling et al.81 N/A FISH-quant v2 big-FISH package Imbert et al.82 N/A Mitochondria Analyzer Chaudhry et al.83 N/A empanada-napari Conrad and Narayan84 N/A Other Anti-Flag M2 magnetic beads Sigma M8823 e2 Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 .. Human Bronchial Epithelial Cells (HBECs) were cultured in Keratinocyte SFM media (Gibco, 17005042) in the presence of 5% CO2 at 37 oC in a humidified incubator.

    Article Title: Large-scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation.
    Article Snippet: Transfections were performed using ViaFect Transfection Reagent (Catalog #E4981; Promega), which is optimized for HEK cells. .. Plasmid DNA encoding BFP (T.gonfio) or a GFP plasmid of similar size (Addgene Plasmid #48138) was mixed with ViaFect in Opti-MEMTM (Catalog # 31985062; Thermo Fisher) following the manufacturer’s instructions. ..

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs
    Article Snippet: Recombinant DNA , , . .. PX458 , Addgene , Plasmid #48138. .. U2AF1 C-terminal 3XFlag Donor plasmid , This study , Addgene Plasmid.

    Article Title: SPCS2 serves as a critical host factor for JEV replication by regulating viral protein stability and virion assembly.
    Article Snippet: .. First, 20 nt oligos were synthesized and annealed prior to insertion into BbsI-digested plasmid pSpCas9BB-2A-GFP (PX458) (Addgene, #48138). ..

    Article Title: A hybrid nanoadjuvant cascading activation of the cGAS-STING-IFN-Ⅰ pathway to enhance radio-immunotherapy.
    Article Snippet: The cGAS-STING signaling pathway is crucial for radiotherapy (RT)-induced immune modulation.. However, under hypoxic conditions, reduced DNA damage and enhanced DNA damage repair (DDR) lead to lower cytosolic double-stranded DNA (dsDNA) levels, making standard RT doses inadequate for sustained cGAS-STING activation, resulting in transient immune responses.. In this study, we developed the As–Mn@MnO2@Alb nanosystem as a potent radiosensitizer and cGAS-STING pathway amplifier.

    Article Title: Compositions for use in treating autosomal dominant BEST1-related retinopathies
    Article Snippet: .. In addition, the px458 plasmid carrying the sgRNA 581G-17nt sequence (SEQ ID NO: 13) expresses both, the SpCas9 and the red fluorescent protein mcherry (Addgene #64324), while the px458 plasmid carrying sgRNA 749A-20nt (SEQ ID NO: 55) expresses the SpCas9 as well as the green fluorescent protein GFP (Addgene #48138). ..

    Luciferase:

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Flag Sigma F3165; RRID: AB_259529 TOMM22 ThermoFisher PA5-51804; RRID AB_2648810 U2AF2 Santa Cruz sc-53942; RRID: AB_831787 EIF3 Bethyl A301-746A-M GIGYF2 Bethyl A303-732A G3BP1 Invitrogen PA5-29455; RRID: AB_2546931 RPS19 Bethyl A304-002A BETA ACTIN Sigma A2228; RRID: AB_476697 HSPD1 Sigma HPA050025; RRID: AB_2680995 Alexa Fluor 488-conjugated anti-Puromycin Biolegend 381506; RRID: AB_2927825 CD34 FITC BD 555821; RRID: AB_396150 CD11b PE Beckman Coulter IM2581U; RRID: AB_131334 GlycophorinA BV421 BD 562938; RRID: AB_2721016 Anti-Puromycin AlexaFluor647 PMY-2A4 Donated by Jonthan Yewdell RRID:AB_2619605 Anti-Puromycin 2A4 AlexaFluor647 Biolegend 381507; RRID: AB_2927826 Biological samples Human bone marrow mono-nuclear primary samples Tampa General Hospital–University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) and Lombardi Cancer Institute, School of Medicine, Georgetown University; Washington, D.C. N/A Chemicals, peptides, and recombinant proteins 3XFlag peptide Sigma F4799 Puromycin Sigma P4512-1MLX10 2-Deoxy-D-glucose (2DG) Sigma D8375-5G Cycloheximide Sigma 239764-1GM Oligomycin Sigma 04876 Critical commercial assays LIVE/DEADTM Fixable Near-IR: Dead Cell Stain Kit Thermo Fisher L10119 EasySepTM Human CD34 Positive Selection Kit II STEMCELL Technologies 17856 Mitochondria Isolation Kit for Cultured Cells Thermo Fisher 89874 EasyPep kit Thermo Fisher A40006 HiScribe T7 RNA synthesis kit NEB E2040S Monarch RNA cleanup kit NEB T2040L DNA Clean & Concentrator Kit Zymo D4003 HiScribe SP6 RNA synthesis kit NEB E2070S Nano-Glo Luciferase Assay Promega N1110 MitoTracker Red CMXRos Invitrogen 2351994 FoxP3 Staining Buffer Set Thermo Fisher 00-5523-00 ImProm-II reverse transcription system Promega A3800 (Continued on next page) Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Mass spec This study MassIVE: MSV000100675 Ribo-sequencing This study GEO: GSE318055 RBNS Donavanet al.49 N/A Experimental models: Cell lines HBEC3-KT U2AF1wt/wt cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/S34F cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/wt-3XFlag cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) This study N/A Oligonucleotides sgRNA and primers for Crispr/CAS9 genomic engineering IDT Star Methods RBNS primers IDT Star Methods Recombinant DNA PX458 Addgene Plasmid #48138 U2AF1 C-terminal 3XFlag Donor plasmid This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-ΔCDs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT:1-126bp This study Addgene Plasmid Nano luciferase in vitro reporter: 1xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: 2xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs:1–126bp This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_wt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_mt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid In vivo reporter: ATP5F1B-mcherry Addgene # 232783 In vivo reporter: ATP5F1B-mcherry-mtAG This study Addgene Plasmid Software and algorithms Gene Set Enrichment Analysis (GSEA) Subramanian et al.33 N/A Fiji, open-source image processing package github.com/fiji/fiji N/A Bowtie Langmead et al.73 N/A wavClusteR Comoglio et al.74 N/A Enrichr online resource Kuleshov et al.75 N/A deeptools Ramı́rez et al.76 N/A RBNS pipeline Lambert, Robertson et al.77 N/A Probound Rube et al.78 N/A FIMO algorithm from the meme suite Grant et al.43 N/A cutadapt Kechin et al.79 N/A Bowtie2 Langmead and Salzberg80 N/A CellProfiler Stirling et al.81 N/A FISH-quant v2 big-FISH package Imbert et al.82 N/A Mitochondria Analyzer Chaudhry et al.83 N/A empanada-napari Conrad and Narayan84 N/A Other Anti-Flag M2 magnetic beads Sigma M8823 e2 Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 .. Human Bronchial Epithelial Cells (HBECs) were cultured in Keratinocyte SFM media (Gibco, 17005042) in the presence of 5% CO2 at 37 oC in a humidified incubator.

    In Vitro:

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Flag Sigma F3165; RRID: AB_259529 TOMM22 ThermoFisher PA5-51804; RRID AB_2648810 U2AF2 Santa Cruz sc-53942; RRID: AB_831787 EIF3 Bethyl A301-746A-M GIGYF2 Bethyl A303-732A G3BP1 Invitrogen PA5-29455; RRID: AB_2546931 RPS19 Bethyl A304-002A BETA ACTIN Sigma A2228; RRID: AB_476697 HSPD1 Sigma HPA050025; RRID: AB_2680995 Alexa Fluor 488-conjugated anti-Puromycin Biolegend 381506; RRID: AB_2927825 CD34 FITC BD 555821; RRID: AB_396150 CD11b PE Beckman Coulter IM2581U; RRID: AB_131334 GlycophorinA BV421 BD 562938; RRID: AB_2721016 Anti-Puromycin AlexaFluor647 PMY-2A4 Donated by Jonthan Yewdell RRID:AB_2619605 Anti-Puromycin 2A4 AlexaFluor647 Biolegend 381507; RRID: AB_2927826 Biological samples Human bone marrow mono-nuclear primary samples Tampa General Hospital–University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) and Lombardi Cancer Institute, School of Medicine, Georgetown University; Washington, D.C. N/A Chemicals, peptides, and recombinant proteins 3XFlag peptide Sigma F4799 Puromycin Sigma P4512-1MLX10 2-Deoxy-D-glucose (2DG) Sigma D8375-5G Cycloheximide Sigma 239764-1GM Oligomycin Sigma 04876 Critical commercial assays LIVE/DEADTM Fixable Near-IR: Dead Cell Stain Kit Thermo Fisher L10119 EasySepTM Human CD34 Positive Selection Kit II STEMCELL Technologies 17856 Mitochondria Isolation Kit for Cultured Cells Thermo Fisher 89874 EasyPep kit Thermo Fisher A40006 HiScribe T7 RNA synthesis kit NEB E2040S Monarch RNA cleanup kit NEB T2040L DNA Clean & Concentrator Kit Zymo D4003 HiScribe SP6 RNA synthesis kit NEB E2070S Nano-Glo Luciferase Assay Promega N1110 MitoTracker Red CMXRos Invitrogen 2351994 FoxP3 Staining Buffer Set Thermo Fisher 00-5523-00 ImProm-II reverse transcription system Promega A3800 (Continued on next page) Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Mass spec This study MassIVE: MSV000100675 Ribo-sequencing This study GEO: GSE318055 RBNS Donavanet al.49 N/A Experimental models: Cell lines HBEC3-KT U2AF1wt/wt cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/S34F cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/wt-3XFlag cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) This study N/A Oligonucleotides sgRNA and primers for Crispr/CAS9 genomic engineering IDT Star Methods RBNS primers IDT Star Methods Recombinant DNA PX458 Addgene Plasmid #48138 U2AF1 C-terminal 3XFlag Donor plasmid This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-ΔCDs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT:1-126bp This study Addgene Plasmid Nano luciferase in vitro reporter: 1xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: 2xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs:1–126bp This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_wt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_mt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid In vivo reporter: ATP5F1B-mcherry Addgene # 232783 In vivo reporter: ATP5F1B-mcherry-mtAG This study Addgene Plasmid Software and algorithms Gene Set Enrichment Analysis (GSEA) Subramanian et al.33 N/A Fiji, open-source image processing package github.com/fiji/fiji N/A Bowtie Langmead et al.73 N/A wavClusteR Comoglio et al.74 N/A Enrichr online resource Kuleshov et al.75 N/A deeptools Ramı́rez et al.76 N/A RBNS pipeline Lambert, Robertson et al.77 N/A Probound Rube et al.78 N/A FIMO algorithm from the meme suite Grant et al.43 N/A cutadapt Kechin et al.79 N/A Bowtie2 Langmead and Salzberg80 N/A CellProfiler Stirling et al.81 N/A FISH-quant v2 big-FISH package Imbert et al.82 N/A Mitochondria Analyzer Chaudhry et al.83 N/A empanada-napari Conrad and Narayan84 N/A Other Anti-Flag M2 magnetic beads Sigma M8823 e2 Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 .. Human Bronchial Epithelial Cells (HBECs) were cultured in Keratinocyte SFM media (Gibco, 17005042) in the presence of 5% CO2 at 37 oC in a humidified incubator.

    In Vivo:

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Flag Sigma F3165; RRID: AB_259529 TOMM22 ThermoFisher PA5-51804; RRID AB_2648810 U2AF2 Santa Cruz sc-53942; RRID: AB_831787 EIF3 Bethyl A301-746A-M GIGYF2 Bethyl A303-732A G3BP1 Invitrogen PA5-29455; RRID: AB_2546931 RPS19 Bethyl A304-002A BETA ACTIN Sigma A2228; RRID: AB_476697 HSPD1 Sigma HPA050025; RRID: AB_2680995 Alexa Fluor 488-conjugated anti-Puromycin Biolegend 381506; RRID: AB_2927825 CD34 FITC BD 555821; RRID: AB_396150 CD11b PE Beckman Coulter IM2581U; RRID: AB_131334 GlycophorinA BV421 BD 562938; RRID: AB_2721016 Anti-Puromycin AlexaFluor647 PMY-2A4 Donated by Jonthan Yewdell RRID:AB_2619605 Anti-Puromycin 2A4 AlexaFluor647 Biolegend 381507; RRID: AB_2927826 Biological samples Human bone marrow mono-nuclear primary samples Tampa General Hospital–University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) and Lombardi Cancer Institute, School of Medicine, Georgetown University; Washington, D.C. N/A Chemicals, peptides, and recombinant proteins 3XFlag peptide Sigma F4799 Puromycin Sigma P4512-1MLX10 2-Deoxy-D-glucose (2DG) Sigma D8375-5G Cycloheximide Sigma 239764-1GM Oligomycin Sigma 04876 Critical commercial assays LIVE/DEADTM Fixable Near-IR: Dead Cell Stain Kit Thermo Fisher L10119 EasySepTM Human CD34 Positive Selection Kit II STEMCELL Technologies 17856 Mitochondria Isolation Kit for Cultured Cells Thermo Fisher 89874 EasyPep kit Thermo Fisher A40006 HiScribe T7 RNA synthesis kit NEB E2040S Monarch RNA cleanup kit NEB T2040L DNA Clean & Concentrator Kit Zymo D4003 HiScribe SP6 RNA synthesis kit NEB E2070S Nano-Glo Luciferase Assay Promega N1110 MitoTracker Red CMXRos Invitrogen 2351994 FoxP3 Staining Buffer Set Thermo Fisher 00-5523-00 ImProm-II reverse transcription system Promega A3800 (Continued on next page) Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Mass spec This study MassIVE: MSV000100675 Ribo-sequencing This study GEO: GSE318055 RBNS Donavanet al.49 N/A Experimental models: Cell lines HBEC3-KT U2AF1wt/wt cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/S34F cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/wt-3XFlag cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) This study N/A Oligonucleotides sgRNA and primers for Crispr/CAS9 genomic engineering IDT Star Methods RBNS primers IDT Star Methods Recombinant DNA PX458 Addgene Plasmid #48138 U2AF1 C-terminal 3XFlag Donor plasmid This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-ΔCDs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT:1-126bp This study Addgene Plasmid Nano luciferase in vitro reporter: 1xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: 2xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs:1–126bp This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_wt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_mt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid In vivo reporter: ATP5F1B-mcherry Addgene # 232783 In vivo reporter: ATP5F1B-mcherry-mtAG This study Addgene Plasmid Software and algorithms Gene Set Enrichment Analysis (GSEA) Subramanian et al.33 N/A Fiji, open-source image processing package github.com/fiji/fiji N/A Bowtie Langmead et al.73 N/A wavClusteR Comoglio et al.74 N/A Enrichr online resource Kuleshov et al.75 N/A deeptools Ramı́rez et al.76 N/A RBNS pipeline Lambert, Robertson et al.77 N/A Probound Rube et al.78 N/A FIMO algorithm from the meme suite Grant et al.43 N/A cutadapt Kechin et al.79 N/A Bowtie2 Langmead and Salzberg80 N/A CellProfiler Stirling et al.81 N/A FISH-quant v2 big-FISH package Imbert et al.82 N/A Mitochondria Analyzer Chaudhry et al.83 N/A empanada-napari Conrad and Narayan84 N/A Other Anti-Flag M2 magnetic beads Sigma M8823 e2 Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 .. Human Bronchial Epithelial Cells (HBECs) were cultured in Keratinocyte SFM media (Gibco, 17005042) in the presence of 5% CO2 at 37 oC in a humidified incubator.

    Software:

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Flag Sigma F3165; RRID: AB_259529 TOMM22 ThermoFisher PA5-51804; RRID AB_2648810 U2AF2 Santa Cruz sc-53942; RRID: AB_831787 EIF3 Bethyl A301-746A-M GIGYF2 Bethyl A303-732A G3BP1 Invitrogen PA5-29455; RRID: AB_2546931 RPS19 Bethyl A304-002A BETA ACTIN Sigma A2228; RRID: AB_476697 HSPD1 Sigma HPA050025; RRID: AB_2680995 Alexa Fluor 488-conjugated anti-Puromycin Biolegend 381506; RRID: AB_2927825 CD34 FITC BD 555821; RRID: AB_396150 CD11b PE Beckman Coulter IM2581U; RRID: AB_131334 GlycophorinA BV421 BD 562938; RRID: AB_2721016 Anti-Puromycin AlexaFluor647 PMY-2A4 Donated by Jonthan Yewdell RRID:AB_2619605 Anti-Puromycin 2A4 AlexaFluor647 Biolegend 381507; RRID: AB_2927826 Biological samples Human bone marrow mono-nuclear primary samples Tampa General Hospital–University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) and Lombardi Cancer Institute, School of Medicine, Georgetown University; Washington, D.C. N/A Chemicals, peptides, and recombinant proteins 3XFlag peptide Sigma F4799 Puromycin Sigma P4512-1MLX10 2-Deoxy-D-glucose (2DG) Sigma D8375-5G Cycloheximide Sigma 239764-1GM Oligomycin Sigma 04876 Critical commercial assays LIVE/DEADTM Fixable Near-IR: Dead Cell Stain Kit Thermo Fisher L10119 EasySepTM Human CD34 Positive Selection Kit II STEMCELL Technologies 17856 Mitochondria Isolation Kit for Cultured Cells Thermo Fisher 89874 EasyPep kit Thermo Fisher A40006 HiScribe T7 RNA synthesis kit NEB E2040S Monarch RNA cleanup kit NEB T2040L DNA Clean & Concentrator Kit Zymo D4003 HiScribe SP6 RNA synthesis kit NEB E2070S Nano-Glo Luciferase Assay Promega N1110 MitoTracker Red CMXRos Invitrogen 2351994 FoxP3 Staining Buffer Set Thermo Fisher 00-5523-00 ImProm-II reverse transcription system Promega A3800 (Continued on next page) Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Mass spec This study MassIVE: MSV000100675 Ribo-sequencing This study GEO: GSE318055 RBNS Donavanet al.49 N/A Experimental models: Cell lines HBEC3-KT U2AF1wt/wt cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/S34F cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/wt-3XFlag cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) This study N/A Oligonucleotides sgRNA and primers for Crispr/CAS9 genomic engineering IDT Star Methods RBNS primers IDT Star Methods Recombinant DNA PX458 Addgene Plasmid #48138 U2AF1 C-terminal 3XFlag Donor plasmid This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-ΔCDs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT:1-126bp This study Addgene Plasmid Nano luciferase in vitro reporter: 1xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: 2xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs:1–126bp This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_wt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_mt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid In vivo reporter: ATP5F1B-mcherry Addgene # 232783 In vivo reporter: ATP5F1B-mcherry-mtAG This study Addgene Plasmid Software and algorithms Gene Set Enrichment Analysis (GSEA) Subramanian et al.33 N/A Fiji, open-source image processing package github.com/fiji/fiji N/A Bowtie Langmead et al.73 N/A wavClusteR Comoglio et al.74 N/A Enrichr online resource Kuleshov et al.75 N/A deeptools Ramı́rez et al.76 N/A RBNS pipeline Lambert, Robertson et al.77 N/A Probound Rube et al.78 N/A FIMO algorithm from the meme suite Grant et al.43 N/A cutadapt Kechin et al.79 N/A Bowtie2 Langmead and Salzberg80 N/A CellProfiler Stirling et al.81 N/A FISH-quant v2 big-FISH package Imbert et al.82 N/A Mitochondria Analyzer Chaudhry et al.83 N/A empanada-napari Conrad and Narayan84 N/A Other Anti-Flag M2 magnetic beads Sigma M8823 e2 Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 .. Human Bronchial Epithelial Cells (HBECs) were cultured in Keratinocyte SFM media (Gibco, 17005042) in the presence of 5% CO2 at 37 oC in a humidified incubator.

    Fluorescence In Situ Hybridization:

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Flag Sigma F3165; RRID: AB_259529 TOMM22 ThermoFisher PA5-51804; RRID AB_2648810 U2AF2 Santa Cruz sc-53942; RRID: AB_831787 EIF3 Bethyl A301-746A-M GIGYF2 Bethyl A303-732A G3BP1 Invitrogen PA5-29455; RRID: AB_2546931 RPS19 Bethyl A304-002A BETA ACTIN Sigma A2228; RRID: AB_476697 HSPD1 Sigma HPA050025; RRID: AB_2680995 Alexa Fluor 488-conjugated anti-Puromycin Biolegend 381506; RRID: AB_2927825 CD34 FITC BD 555821; RRID: AB_396150 CD11b PE Beckman Coulter IM2581U; RRID: AB_131334 GlycophorinA BV421 BD 562938; RRID: AB_2721016 Anti-Puromycin AlexaFluor647 PMY-2A4 Donated by Jonthan Yewdell RRID:AB_2619605 Anti-Puromycin 2A4 AlexaFluor647 Biolegend 381507; RRID: AB_2927826 Biological samples Human bone marrow mono-nuclear primary samples Tampa General Hospital–University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) and Lombardi Cancer Institute, School of Medicine, Georgetown University; Washington, D.C. N/A Chemicals, peptides, and recombinant proteins 3XFlag peptide Sigma F4799 Puromycin Sigma P4512-1MLX10 2-Deoxy-D-glucose (2DG) Sigma D8375-5G Cycloheximide Sigma 239764-1GM Oligomycin Sigma 04876 Critical commercial assays LIVE/DEADTM Fixable Near-IR: Dead Cell Stain Kit Thermo Fisher L10119 EasySepTM Human CD34 Positive Selection Kit II STEMCELL Technologies 17856 Mitochondria Isolation Kit for Cultured Cells Thermo Fisher 89874 EasyPep kit Thermo Fisher A40006 HiScribe T7 RNA synthesis kit NEB E2040S Monarch RNA cleanup kit NEB T2040L DNA Clean & Concentrator Kit Zymo D4003 HiScribe SP6 RNA synthesis kit NEB E2070S Nano-Glo Luciferase Assay Promega N1110 MitoTracker Red CMXRos Invitrogen 2351994 FoxP3 Staining Buffer Set Thermo Fisher 00-5523-00 ImProm-II reverse transcription system Promega A3800 (Continued on next page) Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Mass spec This study MassIVE: MSV000100675 Ribo-sequencing This study GEO: GSE318055 RBNS Donavanet al.49 N/A Experimental models: Cell lines HBEC3-KT U2AF1wt/wt cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/S34F cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/wt-3XFlag cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) This study N/A Oligonucleotides sgRNA and primers for Crispr/CAS9 genomic engineering IDT Star Methods RBNS primers IDT Star Methods Recombinant DNA PX458 Addgene Plasmid #48138 U2AF1 C-terminal 3XFlag Donor plasmid This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-ΔCDs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT:1-126bp This study Addgene Plasmid Nano luciferase in vitro reporter: 1xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: 2xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs:1–126bp This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_wt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_mt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid In vivo reporter: ATP5F1B-mcherry Addgene # 232783 In vivo reporter: ATP5F1B-mcherry-mtAG This study Addgene Plasmid Software and algorithms Gene Set Enrichment Analysis (GSEA) Subramanian et al.33 N/A Fiji, open-source image processing package github.com/fiji/fiji N/A Bowtie Langmead et al.73 N/A wavClusteR Comoglio et al.74 N/A Enrichr online resource Kuleshov et al.75 N/A deeptools Ramı́rez et al.76 N/A RBNS pipeline Lambert, Robertson et al.77 N/A Probound Rube et al.78 N/A FIMO algorithm from the meme suite Grant et al.43 N/A cutadapt Kechin et al.79 N/A Bowtie2 Langmead and Salzberg80 N/A CellProfiler Stirling et al.81 N/A FISH-quant v2 big-FISH package Imbert et al.82 N/A Mitochondria Analyzer Chaudhry et al.83 N/A empanada-napari Conrad and Narayan84 N/A Other Anti-Flag M2 magnetic beads Sigma M8823 e2 Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 .. Human Bronchial Epithelial Cells (HBECs) were cultured in Keratinocyte SFM media (Gibco, 17005042) in the presence of 5% CO2 at 37 oC in a humidified incubator.

    Magnetic Beads:

    Article Title: U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Flag Sigma F3165; RRID: AB_259529 TOMM22 ThermoFisher PA5-51804; RRID AB_2648810 U2AF2 Santa Cruz sc-53942; RRID: AB_831787 EIF3 Bethyl A301-746A-M GIGYF2 Bethyl A303-732A G3BP1 Invitrogen PA5-29455; RRID: AB_2546931 RPS19 Bethyl A304-002A BETA ACTIN Sigma A2228; RRID: AB_476697 HSPD1 Sigma HPA050025; RRID: AB_2680995 Alexa Fluor 488-conjugated anti-Puromycin Biolegend 381506; RRID: AB_2927825 CD34 FITC BD 555821; RRID: AB_396150 CD11b PE Beckman Coulter IM2581U; RRID: AB_131334 GlycophorinA BV421 BD 562938; RRID: AB_2721016 Anti-Puromycin AlexaFluor647 PMY-2A4 Donated by Jonthan Yewdell RRID:AB_2619605 Anti-Puromycin 2A4 AlexaFluor647 Biolegend 381507; RRID: AB_2927826 Biological samples Human bone marrow mono-nuclear primary samples Tampa General Hospital–University of South Florida Health Precision Medicine Biorepository (RRID:SCR_027501) and Lombardi Cancer Institute, School of Medicine, Georgetown University; Washington, D.C. N/A Chemicals, peptides, and recombinant proteins 3XFlag peptide Sigma F4799 Puromycin Sigma P4512-1MLX10 2-Deoxy-D-glucose (2DG) Sigma D8375-5G Cycloheximide Sigma 239764-1GM Oligomycin Sigma 04876 Critical commercial assays LIVE/DEADTM Fixable Near-IR: Dead Cell Stain Kit Thermo Fisher L10119 EasySepTM Human CD34 Positive Selection Kit II STEMCELL Technologies 17856 Mitochondria Isolation Kit for Cultured Cells Thermo Fisher 89874 EasyPep kit Thermo Fisher A40006 HiScribe T7 RNA synthesis kit NEB E2040S Monarch RNA cleanup kit NEB T2040L DNA Clean & Concentrator Kit Zymo D4003 HiScribe SP6 RNA synthesis kit NEB E2070S Nano-Glo Luciferase Assay Promega N1110 MitoTracker Red CMXRos Invitrogen 2351994 FoxP3 Staining Buffer Set Thermo Fisher 00-5523-00 ImProm-II reverse transcription system Promega A3800 (Continued on next page) Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Mass spec This study MassIVE: MSV000100675 Ribo-sequencing This study GEO: GSE318055 RBNS Donavanet al.49 N/A Experimental models: Cell lines HBEC3-KT U2AF1wt/wt cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/S34F cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) Dr. Harold Varmus lab N/A HBEC3-KT U2AF1wt/wt-3XFlag cells (normal human bronchial epithelial cells immortalized with CDK4 and hTERT) This study N/A Oligonucleotides sgRNA and primers for Crispr/CAS9 genomic engineering IDT Star Methods RBNS primers IDT Star Methods Recombinant DNA PX458 Addgene Plasmid #48138 U2AF1 C-terminal 3XFlag Donor plasmid This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-ΔCDs This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-WT:1-126bp This study Addgene Plasmid Nano luciferase in vitro reporter: 1xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: 2xHA-MCL1 This study Addgene Plasmid Nano luciferase in vitro reporter: MCL1-mtAGs:1–126bp This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_wt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid Nano luciferase in vitro reporter: ATP5F1B_mt_Truncated_Nano-Luc_Reporter This study Addgene Plasmid In vivo reporter: ATP5F1B-mcherry Addgene # 232783 In vivo reporter: ATP5F1B-mcherry-mtAG This study Addgene Plasmid Software and algorithms Gene Set Enrichment Analysis (GSEA) Subramanian et al.33 N/A Fiji, open-source image processing package github.com/fiji/fiji N/A Bowtie Langmead et al.73 N/A wavClusteR Comoglio et al.74 N/A Enrichr online resource Kuleshov et al.75 N/A deeptools Ramı́rez et al.76 N/A RBNS pipeline Lambert, Robertson et al.77 N/A Probound Rube et al.78 N/A FIMO algorithm from the meme suite Grant et al.43 N/A cutadapt Kechin et al.79 N/A Bowtie2 Langmead and Salzberg80 N/A CellProfiler Stirling et al.81 N/A FISH-quant v2 big-FISH package Imbert et al.82 N/A Mitochondria Analyzer Chaudhry et al.83 N/A empanada-napari Conrad and Narayan84 N/A Other Anti-Flag M2 magnetic beads Sigma M8823 e2 Molecular Cell 86, 1293–1310.e1–e14, April 2, 2026 .. Human Bronchial Epithelial Cells (HBECs) were cultured in Keratinocyte SFM media (Gibco, 17005042) in the presence of 5% CO2 at 37 oC in a humidified incubator.

    Gene Knockout:

    Article Title: Matrix stiffness induces midnolin-dependent lamin B1 degradation to control myoblast differentiation.
    Article Snippet: Samples were run on a QuantStudio 3 Real-time PCR System (Applied Biosystems). .. CRISPR-mediated lamin B1 gene knockout To knockout lamin B1 genes, we first inserted mLmnb1 gRNA into pSpCas9(BB)-2A-GFP (PX458) (addgene#48138). ..

    Knock-Out:

    Article Title: Matrix stiffness induces midnolin-dependent lamin B1 degradation to control myoblast differentiation.
    Article Snippet: Samples were run on a QuantStudio 3 Real-time PCR System (Applied Biosystems). .. CRISPR-mediated lamin B1 gene knockout To knockout lamin B1 genes, we first inserted mLmnb1 gRNA into pSpCas9(BB)-2A-GFP (PX458) (addgene#48138). ..

    Synthesized:

    Article Title: SPCS2 serves as a critical host factor for JEV replication by regulating viral protein stability and virion assembly.
    Article Snippet: .. First, 20 nt oligos were synthesized and annealed prior to insertion into BbsI-digested plasmid pSpCas9BB-2A-GFP (PX458) (Addgene, #48138). ..

    Clone Assay:

    Article Title: A hybrid nanoadjuvant cascading activation of the cGAS-STING-IFN-Ⅰ pathway to enhance radio-immunotherapy.
    Article Snippet: The cGAS-STING signaling pathway is crucial for radiotherapy (RT)-induced immune modulation.. However, under hypoxic conditions, reduced DNA damage and enhanced DNA damage repair (DDR) lead to lower cytosolic double-stranded DNA (dsDNA) levels, making standard RT doses inadequate for sustained cGAS-STING activation, resulting in transient immune responses.. In this study, we developed the As–Mn@MnO2@Alb nanosystem as a potent radiosensitizer and cGAS-STING pathway amplifier.

    Sequencing:

    Article Title: Compositions for use in treating autosomal dominant BEST1-related retinopathies
    Article Snippet: .. In addition, the px458 plasmid carrying the sgRNA 581G-17nt sequence (SEQ ID NO: 13) expresses both, the SpCas9 and the red fluorescent protein mcherry (Addgene #64324), while the px458 plasmid carrying sgRNA 749A-20nt (SEQ ID NO: 55) expresses the SpCas9 as well as the green fluorescent protein GFP (Addgene #48138). ..



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    Image Search Results


    List of tested (white fields) and reference genes.

    Journal: International Journal of Molecular Sciences

    Article Title: Biosynthesis of Phenolic Compounds of Medicago truncatula After Inoculation with Selected PGPR Strains

    doi: 10.3390/ijms252312684

    Figure Lengend Snippet: List of tested (white fields) and reference genes.

    Article Snippet: PCR primers for both semi-quantitative and quantitative methods were designed using Primer Express 3.0 software (Applied Biosystems, Carlsbad, CA, USA)— .

    Techniques: Sequencing