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downstream and rt primers of the stem-loop method  (Sangon Biotech)

 
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    Structured Review

    Sangon Biotech downstream and rt primers of the stem-loop method
    Downstream And Rt Primers Of The Stem Loop Method, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer+method/stem+loop+primers/pm40327646-110-7-13
    Average 90 stars, based on 1 article reviews
    downstream and rt primers of the stem-loop method - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Analysis of the Expression Patterns of piRNAs in Response to Microsporidian Invasion in Midgut of Workers ( Apis cerana cerana ).
    Article Snippet: Specific stem-loop primers along with upstream primers (F) and universal downstream primers (R) (Table S1) were designed using DNAMAN software (Version: 8.0.8.789) and synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).

    Article Title: ame-miR-5119- Eth axis modulates larval-pupal transition of western honeybee worker
    Article Snippet: Specific stem-loop primers and forward primers (F) as well as universal reverse primers (R) of ame-miR-5119 were designed using DNAMAN software ( ) and then synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).

    Article Title: The up-regulation of TGF-β1 by miRNA-132-3p/WT1 is involved in inducing leukemia cells to differentiate into macrophages.
    Article Snippet: The downstream and RT primers of the stem-loop method were artificially added (Shanghai Sangon Biotech, Co., Ltd.).

    Article Title: One-Pot Sequential Enrichment of Urinary Extracellular Vesicle and miRNAs Identifies a Noninvasive Biomarker Panel for Prostate Cancer Diagnosis.
    Article Snippet: Extracellular vesicles (EVs) offer promising noninvasive alternatives for convenient and noninvasive prostate cancer (PCa) diagnosis, but inefficient EV enrichment and cargo extraction hinder discovery and validation for their clinical applications.. Here, we present an integrated pipeline based on functionalized magnetic beads to streamline and enhance the efficiency of urinary EV miRNA analysis.. EVs are first enriched on amphiphilic magnetic beads through chemical affinity, followed by EV lysis and the isolation of miRNAs through solid phase extraction.

    Article Title: Analysis of the Expression Patterns of piRNAs in Response to Microsporidian Invasion in Midgut of Workers ( Apis cerana cerana )
    Article Snippet: Specific stem-loop primers along with upstream primers (F) and universal downstream primers (R) ( ) were designed using DNAMAN software (Version: 8.0.8.789) and synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).

    Article Title: Global discovery, expression pattern, and regulatory role of miRNA-like RNAs in Ascosphaera apis infecting the Asian honeybee larvae
    Article Snippet: Then, specific Stem-loop primers and upstream primers (F) as well as universal downstream primers (R) were designed and synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) ( ).

    shRNA:

    Article Title: Neural circuits between nodose ganglion and pulmonary neuroendocrine cells regulate lung inflammatory responses
    Article Snippet: .. Short hairpin RNA (shRNA)-mediated RNA interference was employed, with shRNA sequences designed based on the target gene and synthesized as corresponding stem-loop primers (Sangon Biotech). .. These primers were annealed and inserted into the pLKO.1 plasmid between the EcoRI and NheI restriction sites (CST).

    Synthesized:

    Article Title: Neural circuits between nodose ganglion and pulmonary neuroendocrine cells regulate lung inflammatory responses
    Article Snippet: .. Short hairpin RNA (shRNA)-mediated RNA interference was employed, with shRNA sequences designed based on the target gene and synthesized as corresponding stem-loop primers (Sangon Biotech). .. These primers were annealed and inserted into the pLKO.1 plasmid between the EcoRI and NheI restriction sites (CST).

    Polymerase Chain Reaction:

    Article Title: Expression of miR-223-3p in patients with hepatitis B virus liver fibrosis and its effect on hepatic stellate cells: An observational study
    Article Snippet: .. Stem loop RT primer (5′-GTCGTATCCAGTGCAGGGTCCGAGG, TATTCGCACTGGATACGACTGGGGT-3′), PCR primer (F:5′- GGGGTGTCAGTTTGTCAAA-3’, R:5′-CAGTGCGT GTCGTGGAGT-3′), U6RT primer (5′-AAAATATGGAACGCT-3′), and PCR primer (F:5′-CTCGCTTCGGCAGCACA-3’, R:5′-AACGCTTCACGAATTTGCGT-3′) of has-miR-223-3p were purchased from Sangon Biotech (Shanghai) Co., Ltd. ..



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    List of tested (white fields) and reference genes.

    Journal: International Journal of Molecular Sciences

    Article Title: Biosynthesis of Phenolic Compounds of Medicago truncatula After Inoculation with Selected PGPR Strains

    doi: 10.3390/ijms252312684

    Figure Lengend Snippet: List of tested (white fields) and reference genes.

    Article Snippet: PCR primers for both semi-quantitative and quantitative methods were designed using Primer Express 3.0 software (Applied Biosystems, Carlsbad, CA, USA)— .

    Techniques: Sequencing