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Caredx Inc pcr sequencespecific primer method (pcrssp)
Pcr Sequencespecific Primer Method (Pcrssp), supplied by Caredx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+method/pcr+sequence+specific+priming/pm39865484-6-1-9
Average 90 stars, based on 1 article reviews
pcr sequencespecific primer method (pcrssp) - by Bioz Stars, 2026-09
90/100 stars

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Article Title: The novel HLA class II allele, DPB1*1284:01, identified using next-generation sequencing.
Article Snippet: -C, -B, -DRB3/4/5, -DRB1, -DQB1 loci in Zhejiang Han population, China using NGS technology.. Int J Immunogenet.. 2021;48: 485-489.

Article Title: Identification of the Novel HLA-C*06:44:02 Allele by Next-Generation Sequencing in a Serbian Individual.
Article Snippet: The novel HLAC*06:44:02 allele differs from HLAC*06:44:01 by one synonymous nucleotide substitution in exon 2.. In this report, we describe the identification of the novel HLAC*06:44:02 allele, observed in a 66yearold male haematological patient, who was typed for HLA class I (HLAA, B, C) and class II (HLADRB1, DRB3, DRB4, DQA1, DQB1, DPA1, DPB1) by nextgeneration sequencing (NGS) for best matching donor search.. The complete HLA typing of the patient with the novel allele was as follows: HLAA*24:02:01:04, 25:01:01:01; B*35:03:01:01, 57:01:01:01; C*06:44:02, 12:03:01:01; DRB1*07:01:01:01, 14:54:01:01; DRB3*02:02:01:03; DRB4*01:03:01:02N; DQA1*01:04:01:03, 02:01:01:01; DQB1*03:03:02:01, 05:03:01:01; DPA1*01:03:01:05, 02:01:01:06; DPB1*04:02:01:02, 13:01:01:01.

Article Title: Association of human leukocyte antigen class I and class II alleles and haplotypes in COVID-19 infection in a western Indian population.
Article Snippet: We have genotyped HLA class I and II loci in a case-control study of 235 COVID-19 patients and 228 COVID-19 negative healthy controls from Maharashtra, India using a polymerase chain reactionsequence specific primer (PCR-SSP) kit following the manufacturer's instructions (Olerup SSP HLA-A-B-C and Olerup SSP DQ-DR Combi trays of low resolution, CareDx, California, USA).

Article Title: Four novel HLA class II alleles identified using next-generation sequencing.
Article Snippet: Correspondence Noureddine Berka, Histocompatibility and Immunogenetics Laboratory, Department of Pathology and Laboratory Medicine, University of Calgary, Alberta Precision Laboratories, 9-3535 Research Rd NW, Calgary, AB T2L 2K8, Canada.. Email: noureddine.berka@ albertaprecisionlabs.ca We describe here four novel HLA class II alleles, DPA1*01:33, DPB1*1082:01, DPB1*1144:01, and DQA1*01:45, identified in our laboratory following the implementation of next generation sequencing (NGS) for routine high resolution HLA typing of donors and recipients for solid organ transplant and hematopoetic stem cell transplant.. The NGS technology has improved our ability to match at all clinically HLA loci and to facilitate the interpretation of allele specific antibody and eplet matching.

Article Title: Incidence and Role of Recipient-Specific Antibodies in Allogeneic Hematopoietic Cell Transplantation from Mismatched Related Donors.
Article Snippet: Testing was done by the PCR SSP (sequence-specific primer) method (Olerup; CareDx) or next-generation sequencing (NGS) method (MiaFora; Immucor) using the MiSeq system (Illumina).

Article Title: Memory T-cell enriched haploidentical transplantation with NK cell addback results in promising long-term outcomes: a phase II trial
Article Snippet: • Table 1: Outcome in maraviroc patients • Table 2: Graft depletion and recovery • Table 3: Common and notable adverse events • Table 4: Acute GVHD meds used and outcomes • Table 5: Cause of death • Table 6: Complete univariate analysis HAPNK1 protocol 1 SUPPLEMENTARY METHODS KIR typing and selection of alloreactive donors KIR genotyping was performed by sequence specific primers (SSP) (Olerup KIR, CareDx, Brisbane, CA).

Immunopeptidomics:

Article Title: Extended genomic HLA typing identifies previously unrecognized mismatches in living kidney transplantation
Article Snippet: From 2015, serological typing was performed in-house using peripheral blood lymphocytes isolated by gradient centrifugation and typed for HLA-A, -B, and -C using commercial trays (inno-train GmbH, Kronberg, Germany) following the manufacturer’s protocol. .. HLA typing in one-field resolution was performed using PCR sequence specific priming (CareDx, San Francisco, CA, USA) following the manufacturer’s recommendations. ..

Polymerase Chain Reaction:

Article Title: Extended genomic HLA typing identifies previously unrecognized mismatches in living kidney transplantation
Article Snippet: From 2015, serological typing was performed in-house using peripheral blood lymphocytes isolated by gradient centrifugation and typed for HLA-A, -B, and -C using commercial trays (inno-train GmbH, Kronberg, Germany) following the manufacturer’s protocol. .. HLA typing in one-field resolution was performed using PCR sequence specific priming (CareDx, San Francisco, CA, USA) following the manufacturer’s recommendations. ..

Sequencing:

Article Title: Extended genomic HLA typing identifies previously unrecognized mismatches in living kidney transplantation
Article Snippet: From 2015, serological typing was performed in-house using peripheral blood lymphocytes isolated by gradient centrifugation and typed for HLA-A, -B, and -C using commercial trays (inno-train GmbH, Kronberg, Germany) following the manufacturer’s protocol. .. HLA typing in one-field resolution was performed using PCR sequence specific priming (CareDx, San Francisco, CA, USA) following the manufacturer’s recommendations. ..



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List of tested (white fields) and reference genes.
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Image Search Results


List of tested (white fields) and reference genes.

Journal: International Journal of Molecular Sciences

Article Title: Biosynthesis of Phenolic Compounds of Medicago truncatula After Inoculation with Selected PGPR Strains

doi: 10.3390/ijms252312684

Figure Lengend Snippet: List of tested (white fields) and reference genes.

Article Snippet: PCR primers for both semi-quantitative and quantitative methods were designed using Primer Express 3.0 software (Applied Biosystems, Carlsbad, CA, USA)— .

Techniques: Sequencing