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PrimerDesign Inc primer express
Primer Express, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+express+software/primer+express+software/pm35809267-101-9-0
Average 90 stars, based on 1 article reviews
primer express - by Bioz Stars, 2026-11
90/100 stars

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Software:

Article Title: Adjuvant measures and genetic evaluations to improve results of hair transplantation.
Article Snippet: .. Primer design for these two genes was done using Primer Express and Gene runner software. ..

Article Title: Fortification and bioaccessibility of saffron apocarotenoids in potato tubers
Article Snippet: For real-time quantitative reverse transcription (qRT-PCR) analysis, total RNA (2 μg) was treated with RQ1 DNase (Promega, Madrid, Spain) and reverse-transcribed using oligo dT primers, while first-strand cDNAs were synthesized by RT from 1 to 2 μg of total RNA using a first-strand cDNA synthesis kit from GE Healthcare Life Sciences (Buckinghamshire, UK) and 18mer oligo dT. .. Primer design was carried out using the Primer Express software. .. The primer used for the analyses of carotenoid and apocarotenoid was as listed ( ) and , and Real-time PCR was achieved with a StepOneTM Thermal Cycler (Applied Biosystems, Foster City, CA, USA) and analyzed using StepOne software v2.0 (Applied Biosystems, Foster City, CA, USA).

Article Title: PKM2 interacts with and phosphorylates PHB2 to sustain mitochondrial quality control against septic cerebral-cardiac injury
Article Snippet: .. Primer design for qRT-PCR was facilitated by Primer Express software, and the 2 -ΔΔCt method was employed to evaluate relative expression changes in genes and microRNAs . .. HL-1 and N2a cell lines, sourced from ATCC, underwent several passages before experimental use.

Article Title: Inhibition of advanced glycation endproducts formation by lotus seedpod oligomeric procyanidins through RAGE-MAPK signaling and NF-κB activation in high-AGEs-diet mice.
Article Snippet: This study investigated the modulatory effects of lotus seedpod oligomeric procyanidins (LSOPC) on the advanced glycation endproducts (AGEs)-induced liver injury via advanced glycation end-product receptors (RAGE)-mitogen-activated protein kinases (MAPK)-nuclear factor-kappa B (NF-κB) signaling pathways in a mice model. To examine the antioxidation properties of LSOPC, a model of high-AGEs-diet were established using Sprague Dawley (SD) male mice fed with a normal AIN-93G diet, a high AGEs diet (H), or H plus 0.5 or 0.2% (w/ w) LSOPC for 12 weeks.. Our results showed that LSOPC inhibited the AGEs formation and alleviated AGEsinduced liver injury by suppressing the nuclear translocation of NF-κB and activation of the MAPK signaling pathway.. Additionally, LSOPC inhibited the genes expression of tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6).

other:

Article Title: l-norleucine on high glucose-induced insulin sensitivity and mitochondrial function in skeletal muscle cells.
Article Snippet: L-norleucine, an isomer of leucine, stimulates the anabolic process of insulin.. However, it is not known if and how it improves insulin sensitivity and insulin resistance.. This experiment describes the generation of an insulin resistance model using high glucose-induced cells and the administration of 1.0 mmol/L L-norleucine for 48 h, to observe the effects on metabolism and gene expression in skeletal muscle cells.

Article Title: Comparative evaluation of laboratory developed real-time PCR assays and RealStar(®) BKV PCR Kit for quantitative detection of BK polyomavirus.
Article Snippet: Background: Quantitative, viral load monitoring for BK virus (BKV) by real-time PCR is an important tool in the management of polyomavirus associated nephropathy in renal transplant patients.. However, variability in PCR results has been reported because of polymorphisms in viral genes among different subtypes of BKV, and lack of standardization of the PCR assays among different laboratories.. In this study we have compared the performance of several laboratory developed PCR assays that target highly conserved regions of BKV genome with a commercially available, RealStar® BKV PCR Kit.

Quantitative RT-PCR:

Article Title: PKM2 interacts with and phosphorylates PHB2 to sustain mitochondrial quality control against septic cerebral-cardiac injury
Article Snippet: .. Primer design for qRT-PCR was facilitated by Primer Express software, and the 2 -ΔΔCt method was employed to evaluate relative expression changes in genes and microRNAs . .. HL-1 and N2a cell lines, sourced from ATCC, underwent several passages before experimental use.

Expressing:

Article Title: PKM2 interacts with and phosphorylates PHB2 to sustain mitochondrial quality control against septic cerebral-cardiac injury
Article Snippet: .. Primer design for qRT-PCR was facilitated by Primer Express software, and the 2 -ΔΔCt method was employed to evaluate relative expression changes in genes and microRNAs . .. HL-1 and N2a cell lines, sourced from ATCC, underwent several passages before experimental use.



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