tmrm assay kit (Danaher Inc)
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Structured Review
Danaher Inc
tmrm assay kit
Tmrm Assay Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+tmrt+kit/Senescence+Assay+Kit+(Beta+Galactosidase%2C+Fluorescence)+100+tests/pmc09879981-290-0-11
Average 99 stars, based on 112 article reviews
Tmrm Assay Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+tmrt+kit/Senescence+Assay+Kit+(Beta+Galactosidase%2C+Fluorescence)+100+tests/pmc09879981-290-0-11
Average 99 stars, based on 112 article reviews
tmrm assay kit - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "Inhibition of autophagy and MEK promotes ferroptosis in Lkb1-deficient Kras-driven lung tumors"
Article Title: Inhibition of autophagy and MEK promotes ferroptosis in Lkb1-deficient Kras-driven lung tumors
Journal: Cell Death & Disease
doi: 10.1038/s41419-023-05592-8
Figure Legend Snippet: A Clonogenic survival assay of KL (clone 2126 3-2 and clone 2126 5-5) and KP (clone 2871-7 and clone 2871-8) TDCLs treated with HCQ or Trametinib individually at indicated concentrations. B Cell growth inhibition curve of KL (clone 2126 3-2 and clone 2126 5-5) and KP (clone 2871-7 and clone 2871-8) TDCLs treated with HCQ or Trametinib individually at indicated concentrations in Table . C Clonogenic survival assay of KL (clone 2126 3-2 and clone 2126 5-5) and KP (clone 2871-7 and clone 2871-8) TDCLs treated with the combination of HCQ and Trametinib at indicated concentrations. D Relative proliferation of KL (clone 2126 3-2 and clone 2126 5-5) and KP (clone 2871-7 and clone 2871-8) TDCLs treated with vehicle control, HCQ (10 μM), Trametinib (2.5 nM) and the combination. E Western blot for LC3, pERK, total ERK, pS6, total S6 and β-actin of KL and KP TDCLs treated with vehicle control, HCQ (10 μM), Trametinib (2.5 nM) and the combination for 6 h. F Scheme of the KL or KP TDCLs for measuring oxygen consumption rate (OCR) using Seahorse XFe24 analyzer. G Basal respiration and ATP production of KL TDCLs (clone 2126 3-2 and clone 2126 5-5 (with black squares)) after 6 h’ treatment with vehicle control, HCQ (10 μM), Trametinib (2.5 nM) and the combination. H Basal respiration and ATP production of KP TDCLs (clone 2871-1 and clone 2871-8 (with black squares)) after 6 h’ treatment with vehicle control, HCQ (10 μM), Trametinib (2.5 nM) and the combination. I Scheme of the metabolomics analysis via LC-MS of KL TDCLs after 6 h’ treatment. J The levels of metabolites of KL (clone 2126 3-2 and clone 2126 5-5) TDCLs after 6 h’ treatment with vehicle control, HCQ (10 μM), Trametinib (2.5 nM) and the combination. K Left: Overlapping images of KL (clone 2126 3-2 and clone 2126 5-5) TDCLs treated with vehicle control, HCQ (10 μM), Trametinib (2.5 nM) and the combination for 6 h and stained with MitoTracker Red CMXRos for mitochondrial membrane potential and MitoTracker Green FM for mitochondrial mass. Blue: Hoechst 33342 for nuclear staining. Right: graph of the relative mitochondrial membrane potential of KL TDCLs quantified by the ratio of red fluorescence intensity and green fluorescence intensity. L Left: Overlapping images of KP TDCLs (clone 2871-7 and clone 2871-8) treated with vehicle control, HCQ (10 μM), Trametinib (2.5 nM) and the combination for 6 h and stained with MitoTracker Red CMXRos for mitochondrial membrane potential and MitoTracker Green FM for mitochondria mass. Blue: Hoechst 33342 for nuclear staining. Right: graph of relative mitochondrial membrane potential of KP TDCLs quantified by the ratio of red fluorescence intensity and green fluorescence intensity. M Left: Overlapping images of KL (clone 2126 3-2 and clone 2126 5-5) TDCLs treated with vehicle control, HCQ (10 μM), Trametinib (2.5 nM) and the combination for 6 h and stained with TMRM (red fluorescence) for mitochondrial membrane potential. Blue: Hoechst 33342 for nuclear staining. Right: graph of the relative mitochondrial membrane potential of KL TDCLs quantified by the ratio of red fluorescence intensity and total cell numbers. N Left: Overlapping images of KP (clone 2871-1 and clone 2871-8) TDCLs treated with vehicle control, HCQ (10 μM), Trametinib (2.5 nM) and the combination for 6 h and stained with TMRM (red fluorescence) for mitochondrial membrane potential. Blue: Hoechst 33342 for nuclear staining. Right: graph of the relative mitochondrial membrane potential of KP TDCLs quantified by the ratio of red fluorescence intensity and total cell numbers. Data are mean ± s.e.m. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Techniques Used: Clonogenic Cell Survival Assay, Inhibition, Control, Western Blot, Liquid Chromatography with Mass Spectroscopy, Staining, Membrane, Fluorescence
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