tmr red tunel cell apoptosis detection kit (Beijing Solarbio Science)
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Beijing Solarbio Science
tmr red tunel cell apoptosis detection kit
Tmr Red Tunel Cell Apoptosis Detection Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+tmrt+kit/Cell+Apoptosis+Analysis+Kit/pm39773567-100-11-18
Average 96 stars, based on 337 article reviews
Tmr Red Tunel Cell Apoptosis Detection Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+tmrt+kit/Cell+Apoptosis+Analysis+Kit/pm39773567-100-11-18
Average 96 stars, based on 337 article reviews
tmr red tunel cell apoptosis detection kit - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Targeting the NLRP3 inflammasome abrogates cardiotoxicity of immune checkpoint blockers."
Article Title: Targeting the NLRP3 inflammasome abrogates cardiotoxicity of immune checkpoint blockers.
Journal: Journal for immunotherapy of cancer
doi: 10.1136/jitc-2024-010127
Figure Legend Snippet: Figure 2 NLRP3 inhibition attenuates ICI-induced cardiac injury. Mice were inoculated with B16F10 melanoma cells on day 0, and injected with either IgG+vehicle, or αPD-1 antibody+vehicle, or αPD-1 antibody+MCC950 every other day from day 7 to day 19. Hearts were collected on day 20. (A) Experimental design. (B) ELISA for plasma cTnT levels of indicated groups. (C) Echocardiographic analysis showing LVEF, LVFS, LVESV, LVEDV, LVIDs, and LVIDd, in mice of each group. (D, E and F) Representative immunofluorescence images for CD8 (D), F4/80 (E), TUNEL (F) and their statistical analysis of per high-power field (HPF, average of 3–6 different 200× visual fields) in the hearts of each group; scale bar, 50 µm. (G) Representative Masson staining images in mouse hearts of each group and their statistical analysis. Scale bar, 50 µm. (H) RT-qPCR analysis for the expression of Il1b, Il6, Tnf, Ifng, Ccl2, Ccl3, Ccl5. n=8 per group. Data are presented as the mean±SD. Data were analyzed by one-way ANOVA followed by Tukey post hoc multicomparison test. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 compared with indicated group.cTnT, cardiac troponin t; ICI, immune checkpoint inhibitor; LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LVEDV, left ventricular end-diastolic volume; LVESV, left ventricular end-systolic volume; LVIDd, left ventricular internal dimension in diastole; LVIDs, left ventricular internal dimension in systole; RT-qPCR, reverse transcription quantitative polymerase chain reaction; TUNEL, TdT-mediated dUTP nick-end labeling.
Techniques Used: Inhibition, Injection, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Immunofluorescence, TUNEL Assay, Staining, Quantitative RT-PCR, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, End Labeling
Figure Legend Snippet: Figure 6 NLRP3 inhibition ameliorates cardiotoxicity of combined ICI therapy. Mice were inoculated with B16F10 melanoma cells on day 0, and were administrated with IgG+vehicle, or anti-PD-1 + anti-CTLA-4 + vehicle, or anti-PD-1 + anti-CTLA-4 + MCC950 every other day from day 7 to day 19. Hearts were collected on day 20. (A) Experimental design. (B) ELISA for plasma cTnT levels of IgG group, dual ICI (D-ICI) group and dual ICI+MCC950 (D-ICI+MCC950) group. (C) Echocardiographic analysis showing cardiac function of each group. (D, E, and F) Representative immunofluorescence images for CD8 (D), F4/80 (E), TUNEL (F) and their statistical analysis of per high-power field (HPF, average of 3–6 different 200× visual fields) in the heart of each group; scale bar, 50 µm. (G) Representative Masson staining images in mouse hearts of each group and their statistical analysis. Scale bar, 50 µm. (H) RT-qPCR analysis for relative mRNA expression of Il1b, Il6, Tnf, Ifng, Ccl2, Ccl3, Ccl5 in the heart of each group. (I) Tumor volume measurements of the female B16F10 tumor-bearing mice of each group. (J) Tumor volume at day 19 of each group (left) and tumor weight at day 20 of each group (right). n=8 per group. Data are presented as mean±SD. Data were analyzed by one-way ANOVA followed by Tukey post hoc multicomparison test. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 compared with indicated group. RT-qPCR, reverse transcription quantitative polymerase chain reaction; TUNEL, TdT-mediated dUTP nick-end labeling.
Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Immunofluorescence, TUNEL Assay, Staining, Quantitative RT-PCR, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, End Labeling
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