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preservation buffer  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec preservation buffer
    Preservation Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/preservation+buffer/MACS+Tissue+Storage+Solution/pm42011021-60-7-9
    Average 98 stars, based on 886 article reviews
    preservation buffer - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Magnetic Cell Separation:

    Article Title: Cross-species causal gene mapping reveals brain-region-specific targets of Cannabidiol for post-traumatic stress disorder treatment.
    Article Snippet: Purpose: Post-traumatic stress disorder (PTSD) involves hippocampal and prefrontal dysfunction.. Cannabidiol (CBD) shows therapeutic promise, but its cell-type-specific and causal mechanisms remain unclear.. Methods: We performed single-cell RNA sequencing of hippocampal and prefrontal cortices from PTSD mice, healthy controls, and CBD-treated PTSD mice.

    Article Title: Cerebral venous outflow disturbance exacerbates ischemic stroke via an endothelium-initiated inflammatory cascade: an integrated single-cell transcriptomic analysis.
    Article Snippet: .. For scRNA-seq preparation, freshly isolated brain tissues were preserved in MACS Tissue Storage Solution (Miltenyi Biotec, Germany) and mechanically dissociated followed by enzymatic digestion. ..

    Article Title: Single-cell long- and short-read transcriptomics sheds light on high-altitude adaptation in the porcine cerebral cortex.
    Article Snippet: .. The tissue samples were preserved at 4 °C in MACS Tissue Storage Solution (Miltenyi Biotec, Cologne, NW, Germany) for subsequent cell isolation. .. Single-cell suspension preparation Single-cell suspensions from the cerebral cortex of DT and DSE (Sus scrofa) were prepared using the Miltenyi Tumor Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Single-cell mapping of extrachromosomal circular DNA architecture and evolution in cutaneous squamous cell carcinoma.
    Article Snippet: Extrachromosomal circular DNA (eccDNA), characterized by abundant amplicons and high chromatin accessibility, contributes to tumor heterogeneity and transcriptional activity; however, its single-cell dynamics in cutaneous squamous cell carcinoma (CSCC) remain poorly understood.. Here, we developed a single-cell framework eccDNAscope that reconstructs eccDNA structures and infers clonal trajectories from scATAC-seq data.. Integrative analysis of 154,624 single cells revealed that eccDNAs were broadly distributed across diverse cell types but exhibited striking enrichment at promoter regions (79.94%), where they were associated with elevated expression of cell type-specific eccDNA-associated genes.

    Article Title: High SIGLEC10‐Expressing Tumor‐Associated Macrophages Participate in Remodeling the Tumor Immune Microenvironment and Predict Poor Prognosis in Colorectal Cancer
    Article Snippet: The slides were then mounted using antifade mounting medium containing an anti‐fluorescence quenching agent, and representative fields were captured using a Leica DM5000 B microscope (Leica Microsystems, Wetzlar, Germany). .. The freshly collected tumor tissue was rinsed with saline to remove blood and placed in MACS Tissue Storage Solution (Miltenyi Biotec, Germany), then immediately transported to the laboratory on ice. .. Tissue dissociation was performed following the manufacturer's instructions (Miltenyi).

    Article Title: Ion channel/Stat6-driven nano-immune programming of tissue-resident macrophages by amide-functionalized nanocellulose
    Article Snippet: .. The fresh organs were harvested from the mouse and immediately stored using MACS tissue storage solution (Miltenyi Biotech) at 4 °C. ..

    Article Title: The IGF2-ERK-Fos axis drives a Müller cell glial-mesenchymal transition-like response to disrupt retinal homeostasis in early myopia.
    Article Snippet: .. The detached retinal layers were washed twice in tissue washing solution, and the retinas were temporarily stored in MACS® Tissue Storage Solution (Miltenyi Biotec, Germany) at 4°C in order to maintain cell viability. ..

    Preserving:

    Article Title: Single-Cell Transcriptomic Analysis of Tumor Heterogeneity and the Microenvironment in Pseudomyxoma Peritonei.
    Article Snippet: .. Fresh surgical specimens were dissected to isolate representative tumor regions, then immediately placed in pre-chilled tissue preservation solution (130-100-008, Miltenyi) and transported to the laboratory. ..

    Isolation:

    Article Title: Cerebral venous outflow disturbance exacerbates ischemic stroke via an endothelium-initiated inflammatory cascade: an integrated single-cell transcriptomic analysis.
    Article Snippet: .. For scRNA-seq preparation, freshly isolated brain tissues were preserved in MACS Tissue Storage Solution (Miltenyi Biotec, Germany) and mechanically dissociated followed by enzymatic digestion. ..

    Cell Isolation:

    Article Title: Single-cell long- and short-read transcriptomics sheds light on high-altitude adaptation in the porcine cerebral cortex.
    Article Snippet: .. The tissue samples were preserved at 4 °C in MACS Tissue Storage Solution (Miltenyi Biotec, Cologne, NW, Germany) for subsequent cell isolation. .. Single-cell suspension preparation Single-cell suspensions from the cerebral cortex of DT and DSE (Sus scrofa) were prepared using the Miltenyi Tumor Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Sample Prep:

    Article Title: Single-cell mapping of extrachromosomal circular DNA architecture and evolution in cutaneous squamous cell carcinoma.
    Article Snippet: Extrachromosomal circular DNA (eccDNA), characterized by abundant amplicons and high chromatin accessibility, contributes to tumor heterogeneity and transcriptional activity; however, its single-cell dynamics in cutaneous squamous cell carcinoma (CSCC) remain poorly understood.. Here, we developed a single-cell framework eccDNAscope that reconstructs eccDNA structures and infers clonal trajectories from scATAC-seq data.. Integrative analysis of 154,624 single cells revealed that eccDNAs were broadly distributed across diverse cell types but exhibited striking enrichment at promoter regions (79.94%), where they were associated with elevated expression of cell type-specific eccDNA-associated genes.

    Single Cell:

    Article Title: Single-cell mapping of extrachromosomal circular DNA architecture and evolution in cutaneous squamous cell carcinoma.
    Article Snippet: Extrachromosomal circular DNA (eccDNA), characterized by abundant amplicons and high chromatin accessibility, contributes to tumor heterogeneity and transcriptional activity; however, its single-cell dynamics in cutaneous squamous cell carcinoma (CSCC) remain poorly understood.. Here, we developed a single-cell framework eccDNAscope that reconstructs eccDNA structures and infers clonal trajectories from scATAC-seq data.. Integrative analysis of 154,624 single cells revealed that eccDNAs were broadly distributed across diverse cell types but exhibited striking enrichment at promoter regions (79.94%), where they were associated with elevated expression of cell type-specific eccDNA-associated genes.

    Sequencing:

    Article Title: Single-cell mapping of extrachromosomal circular DNA architecture and evolution in cutaneous squamous cell carcinoma.
    Article Snippet: Extrachromosomal circular DNA (eccDNA), characterized by abundant amplicons and high chromatin accessibility, contributes to tumor heterogeneity and transcriptional activity; however, its single-cell dynamics in cutaneous squamous cell carcinoma (CSCC) remain poorly understood.. Here, we developed a single-cell framework eccDNAscope that reconstructs eccDNA structures and infers clonal trajectories from scATAC-seq data.. Integrative analysis of 154,624 single cells revealed that eccDNAs were broadly distributed across diverse cell types but exhibited striking enrichment at promoter regions (79.94%), where they were associated with elevated expression of cell type-specific eccDNA-associated genes.

    Saline:

    Article Title: High SIGLEC10‐Expressing Tumor‐Associated Macrophages Participate in Remodeling the Tumor Immune Microenvironment and Predict Poor Prognosis in Colorectal Cancer
    Article Snippet: The slides were then mounted using antifade mounting medium containing an anti‐fluorescence quenching agent, and representative fields were captured using a Leica DM5000 B microscope (Leica Microsystems, Wetzlar, Germany). .. The freshly collected tumor tissue was rinsed with saline to remove blood and placed in MACS Tissue Storage Solution (Miltenyi Biotec, Germany), then immediately transported to the laboratory on ice. .. Tissue dissociation was performed following the manufacturer's instructions (Miltenyi).



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