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quick 16stm ngs library prep kit  (Zymo Research)


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    Structured Review

    Zymo Research quick 16stm ngs library prep kit
    Quick 16stm Ngs Library Prep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 97/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prep/Quick-16STM+Plus+NGS+Library+P/pmc13129379-192-9-14
    Average 97 stars, based on 88 article reviews
    quick 16stm ngs library prep kit - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: 16S rRNA-based characterization of post-fire soil bacterial communities in a pine-oak forest of northeastern Mexico
    Article Snippet: DNA was extracted from 1 g of soil following the manufacturer’s protocol for the ZymoBIOMICS®-96 MagBead DNA Kit (Zymo Research, Irvine, CA, USA). .. The 16S rRNA gene libraries were prepared using the Quick-16STM Plus NGS Library Prep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s instructions. .. The primers included in the kit (Quick-16STM Primer set V3-V4 Zymo Research, Irvine, CA) were custom-designed to achieve broad gene coverage while maintaining high sensitivity.

    Article Title: Bacterial diversity in ferruginous duricrust (canga) and the physicochemical variables affecting their prevalence, distribution and predicted metabolic pathways.
    Article Snippet: The DNA concentration and purity was measured using a NanoDrop spectrophotometer (NanoDrop Lite Plus, Thermo Fisher Scientific, Waltham, MA, USA). .. PCR amplification and library preparation were performed by Zymo Research Corporation (Botucatu—SP, Brazil) using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA, USA). .. The bacterial 16S primers, custom-designed by Zymo Research, target the V3–V4 region of the 16S rRNA gene to maximize coverage and sensitivity.

    Article Title: Cariogenic microorganisms induce oral epithelial atypia through host-microbiome-high sugar diet interactions.
    Article Snippet: The samples were processed and analyzed with the ZymoBIOMICS® Service in AR TI CL E IN P RE SS Forsyth Institute Sequencing Facility (Cambridge, MA, United States). .. The DNA samples were prepared for targeted sequencing with the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA). .. The final library was sequenced on an Illumina® MiSeqTM with a V3 reagent kit (600 cycles).

    Article Title: Effect of two-doses of 3-nitrooxypropanol (3-NOP) on methane emissions, performance, rumen microbiome, and metabolomics in Nellore cattle
    Article Snippet: In some cases, the ZymoBIOMICS DNA Miniprep Kit or DNA Microprep Kit was used, particularly for low-biomass samples, to ensure higher DNA concentration through reduced elution volumes. .. Bacterial 16S ribosomal RNA gene sequencing was performed using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA). ..

    Article Title: Bacterial diversity in ferruginous duricrust ( canga ) and the physicochemical variables affecting their prevalence, distribution and predicted metabolic pathways
    Article Snippet: The DNA concentration and purity was measured using a NanoDrop spectrophotometer (NanoDrop Lite Plus, Thermo Fisher Scientific, Waltham, MA, USA). .. PCR amplification and library preparation were performed by Zymo Research Corporation (Botucatu—SP, Brazil) using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA, USA). .. The bacterial 16S primers, custom-designed by Zymo Research, target the V3–V4 region of the 16S rRNA gene to maximize coverage and sensitivity.

    Article Title: Anaerobic Treatment of the Liquid Fraction of Food Waste in a Hybrid Reactor with Spatially Structured Biomass: Process Performance and Microbial Community Dynamics
    Article Snippet: The extracted DNA was amplified by polymerase chain reaction (PCR), targeting the V3–V4 region of the 16S rRNA gene using primers recommended by Zymo Research (341F (CCTACGGGDGGCWGCAG/CCTAYGGGGYGCWGCAG) and 806R (GACTACNVGGGTMTCTAATCC)), generating an approximately 460 bp fragment. .. Library preparation was performed with the Quick-16STM Plus NGS Library Prep Kit (V3–V4, Zymo Research), and sequencing was conducted on an Illumina MiSeq platform. .. For comparison, sequencing of the V4 region of the 16S rRNA gene was also performed by Novogene ( www.novogene.com , USA) using primers 515F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT) on an Illumina NovaSeq 6000 platform, generating 100 K paired-end reads.

    Article Title: Utilization of aqueous distillate and solid residue fractions derived from zero-waste Hydrodistillation of Zanthoxylum myriacanthum wall. ex Hook. f. for improving broiler immunity and intestinal functions.
    Article Snippet: The concentration and purity of RNA were assessed via spectrophotometry (Nanodrop ND1000; Thermo Scientific). .. Bacterial 16S ribosomal RNA genes were sequenced with the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA). .. The V3-V4 regions of the 16S rRNA gene were amplified through polymerase chain reaction (PCR) using primers 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′) on an ABI GeneAmp 9700 PCR thermocycler (Thermo Fisher Scientific).

    Article Title: Utilization of aqueous distillate and solid residue fractions derived from zero-waste Hydrodistillation of Zanthoxylum myriacanthum wall. ex Hook. f. for improving broiler immunity and intestinal functions
    Article Snippet: The concentration and purity of RNA were assessed via spectrophotometry (Nanodrop ND1000; Thermo Scientific). .. Bacterial 16S ribosomal RNA genes were sequenced with the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA). .. The V3-V4 regions of the 16S rRNA gene were amplified through polymerase chain reaction (PCR) using primers 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′) on an ABI GeneAmp 9700 PCR thermocycler (Thermo Fisher Scientific).

    Polymerase Chain Reaction:

    Article Title: Bacterial diversity in ferruginous duricrust (canga) and the physicochemical variables affecting their prevalence, distribution and predicted metabolic pathways.
    Article Snippet: The DNA concentration and purity was measured using a NanoDrop spectrophotometer (NanoDrop Lite Plus, Thermo Fisher Scientific, Waltham, MA, USA). .. PCR amplification and library preparation were performed by Zymo Research Corporation (Botucatu—SP, Brazil) using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA, USA). .. The bacterial 16S primers, custom-designed by Zymo Research, target the V3–V4 region of the 16S rRNA gene to maximize coverage and sensitivity.

    Article Title: Bacterial diversity in ferruginous duricrust ( canga ) and the physicochemical variables affecting their prevalence, distribution and predicted metabolic pathways
    Article Snippet: The DNA concentration and purity was measured using a NanoDrop spectrophotometer (NanoDrop Lite Plus, Thermo Fisher Scientific, Waltham, MA, USA). .. PCR amplification and library preparation were performed by Zymo Research Corporation (Botucatu—SP, Brazil) using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA, USA). .. The bacterial 16S primers, custom-designed by Zymo Research, target the V3–V4 region of the 16S rRNA gene to maximize coverage and sensitivity.

    Amplification:

    Article Title: Bacterial diversity in ferruginous duricrust (canga) and the physicochemical variables affecting their prevalence, distribution and predicted metabolic pathways.
    Article Snippet: The DNA concentration and purity was measured using a NanoDrop spectrophotometer (NanoDrop Lite Plus, Thermo Fisher Scientific, Waltham, MA, USA). .. PCR amplification and library preparation were performed by Zymo Research Corporation (Botucatu—SP, Brazil) using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA, USA). .. The bacterial 16S primers, custom-designed by Zymo Research, target the V3–V4 region of the 16S rRNA gene to maximize coverage and sensitivity.

    Article Title: Bacterial diversity in ferruginous duricrust ( canga ) and the physicochemical variables affecting their prevalence, distribution and predicted metabolic pathways
    Article Snippet: The DNA concentration and purity was measured using a NanoDrop spectrophotometer (NanoDrop Lite Plus, Thermo Fisher Scientific, Waltham, MA, USA). .. PCR amplification and library preparation were performed by Zymo Research Corporation (Botucatu—SP, Brazil) using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA, USA). .. The bacterial 16S primers, custom-designed by Zymo Research, target the V3–V4 region of the 16S rRNA gene to maximize coverage and sensitivity.

    Targeted Sequencing:

    Article Title: Cariogenic microorganisms induce oral epithelial atypia through host-microbiome-high sugar diet interactions.
    Article Snippet: The samples were processed and analyzed with the ZymoBIOMICS® Service in AR TI CL E IN P RE SS Forsyth Institute Sequencing Facility (Cambridge, MA, United States). .. The DNA samples were prepared for targeted sequencing with the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA). .. The final library was sequenced on an Illumina® MiSeqTM with a V3 reagent kit (600 cycles).

    Sequencing:

    Article Title: Effect of two-doses of 3-nitrooxypropanol (3-NOP) on methane emissions, performance, rumen microbiome, and metabolomics in Nellore cattle
    Article Snippet: In some cases, the ZymoBIOMICS DNA Miniprep Kit or DNA Microprep Kit was used, particularly for low-biomass samples, to ensure higher DNA concentration through reduced elution volumes. .. Bacterial 16S ribosomal RNA gene sequencing was performed using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA). ..

    Article Title: Anaerobic Treatment of the Liquid Fraction of Food Waste in a Hybrid Reactor with Spatially Structured Biomass: Process Performance and Microbial Community Dynamics
    Article Snippet: The extracted DNA was amplified by polymerase chain reaction (PCR), targeting the V3–V4 region of the 16S rRNA gene using primers recommended by Zymo Research (341F (CCTACGGGDGGCWGCAG/CCTAYGGGGYGCWGCAG) and 806R (GACTACNVGGGTMTCTAATCC)), generating an approximately 460 bp fragment. .. Library preparation was performed with the Quick-16STM Plus NGS Library Prep Kit (V3–V4, Zymo Research), and sequencing was conducted on an Illumina MiSeq platform. .. For comparison, sequencing of the V4 region of the 16S rRNA gene was also performed by Novogene ( www.novogene.com , USA) using primers 515F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT) on an Illumina NovaSeq 6000 platform, generating 100 K paired-end reads.



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