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interferone γ ifn γ  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec interferone γ ifn γ
    Interferone γ Ifn γ, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/premium/Human+IFN-%CE%B31b%2C+premium+grade/pmc12924898-252-19-21
    Average 95 stars, based on 57 article reviews
    interferone γ ifn γ - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Epromoters bind key stress-related transcription factors to regulate clusters of stress response genes
    Article Snippet: Exponentially growing NIH3T3 cells were stimulated by adding one volume of fresh media containing 10 ng/mL TNFα (Abcam, ab9642), for a final concentration of 5 ng/mL, during 4 h. .. To induce macrophage differentiation, THP-1 cells were first plated on 6-well plates (2 × 10 6 cells/well), in media containing 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, P1585) for 48 h. After 48 h of incubation, the PMA-containing media was replaced with fresh media, and cells were incubated for an additional 24 h. THP-1 in vitro differentiated macrophages were then stimulated by replacing media with 2 mL of fresh media containing 100 ng/mL of IFNγ (Miltenyi Biotec, 130-096-484) for 4 h, upon which the extraction of RNA was performed. ..

    Article Title: Epromoters bind key stress-related transcription factors to regulate clusters of stress response genes.
    Article Snippet: Exponentially growing NIH3T3 cells were stimulated by adding one volume of fresh media containing 10 ng/mL TNFα (Abcam, ab9642), for a final concentration of 5 ng/mL, during 4 h. .. To induce macrophage differentiation, THP-1 cells were first plated on 6-well plates (2 × 106 cells/well), in media containing 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, P1585) for 48 h. After 48 h of incubation, the PMA-containing media was replaced with fresh media, and cells were incubated for an additional 24 h. THP-1 in vitro differentiated macrophages were then stimulated by replacing media with 2 mL of fresh media containing 100 ng/mL of IFNγ (Miltenyi Biotec, 130-096-484) for 4 h, upon which the extraction of RNA was performed. ..

    In Vitro:

    Article Title: Epromoters bind key stress-related transcription factors to regulate clusters of stress response genes
    Article Snippet: Exponentially growing NIH3T3 cells were stimulated by adding one volume of fresh media containing 10 ng/mL TNFα (Abcam, ab9642), for a final concentration of 5 ng/mL, during 4 h. .. To induce macrophage differentiation, THP-1 cells were first plated on 6-well plates (2 × 10 6 cells/well), in media containing 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, P1585) for 48 h. After 48 h of incubation, the PMA-containing media was replaced with fresh media, and cells were incubated for an additional 24 h. THP-1 in vitro differentiated macrophages were then stimulated by replacing media with 2 mL of fresh media containing 100 ng/mL of IFNγ (Miltenyi Biotec, 130-096-484) for 4 h, upon which the extraction of RNA was performed. ..

    Article Title: Epromoters bind key stress-related transcription factors to regulate clusters of stress response genes.
    Article Snippet: Exponentially growing NIH3T3 cells were stimulated by adding one volume of fresh media containing 10 ng/mL TNFα (Abcam, ab9642), for a final concentration of 5 ng/mL, during 4 h. .. To induce macrophage differentiation, THP-1 cells were first plated on 6-well plates (2 × 106 cells/well), in media containing 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, P1585) for 48 h. After 48 h of incubation, the PMA-containing media was replaced with fresh media, and cells were incubated for an additional 24 h. THP-1 in vitro differentiated macrophages were then stimulated by replacing media with 2 mL of fresh media containing 100 ng/mL of IFNγ (Miltenyi Biotec, 130-096-484) for 4 h, upon which the extraction of RNA was performed. ..

    Extraction:

    Article Title: Epromoters bind key stress-related transcription factors to regulate clusters of stress response genes
    Article Snippet: Exponentially growing NIH3T3 cells were stimulated by adding one volume of fresh media containing 10 ng/mL TNFα (Abcam, ab9642), for a final concentration of 5 ng/mL, during 4 h. .. To induce macrophage differentiation, THP-1 cells were first plated on 6-well plates (2 × 10 6 cells/well), in media containing 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, P1585) for 48 h. After 48 h of incubation, the PMA-containing media was replaced with fresh media, and cells were incubated for an additional 24 h. THP-1 in vitro differentiated macrophages were then stimulated by replacing media with 2 mL of fresh media containing 100 ng/mL of IFNγ (Miltenyi Biotec, 130-096-484) for 4 h, upon which the extraction of RNA was performed. ..

    Article Title: Epromoters bind key stress-related transcription factors to regulate clusters of stress response genes.
    Article Snippet: Exponentially growing NIH3T3 cells were stimulated by adding one volume of fresh media containing 10 ng/mL TNFα (Abcam, ab9642), for a final concentration of 5 ng/mL, during 4 h. .. To induce macrophage differentiation, THP-1 cells were first plated on 6-well plates (2 × 106 cells/well), in media containing 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, P1585) for 48 h. After 48 h of incubation, the PMA-containing media was replaced with fresh media, and cells were incubated for an additional 24 h. THP-1 in vitro differentiated macrophages were then stimulated by replacing media with 2 mL of fresh media containing 100 ng/mL of IFNγ (Miltenyi Biotec, 130-096-484) for 4 h, upon which the extraction of RNA was performed. ..

    Analysis:

    Article Title: Herring roe PLs promote SPM biosynthesis in macrophages and a keratinocyte/fibroblast coculture as a model of psoriasis
    Article Snippet: MDMs from each donor were treated in 1.5 ml per well in a 6-well plate (3 wells per condition) with indicated final percentages (w/w) of HRO or PEHeRo, by dilution of the prepared emulsions in phenol- and serum-free CTS AIM-V Medium (Gibco, A3830801) supplemented with penicillin-streptomycin. .. MDMs were either pretreated with HRO for 16 h, followed by a change of medium with the addition of 1 μg/ml lipopolysaccharide (LPS, Sigma-Aldrich, L2630) and 50 ng/ml IFN-γ (Miltenyi Biotec, 130-096-481) for a further 24 h or cotreated with either HRO or PEHeRo and 1 ng/ml LPS for 24 h. Supernatants were collected, pooled per condition, centrifuged, and frozen at −80°C until shipping on dry ice for analysis. .. For expression analysis of the enzymes required for SPM formation, MDMs were lysed and RNA isolated by the Quick-RNA Miniprep Kit (Zymo Research, R1054).

    Recombinant:

    Article Title: A transgenic mouse allows characterization of the HLA-C∗06:02 immunopeptidome in a model of psoriasis.
    Article Snippet: Psoriasis vulgaris is a T-cell—mediated autoimmune skin condition affecting around 1 in 50 people worldwide.. Although advanced immunomodulatory therapeutic options have become available in recent years, ongoing disease suppression is still required, with no curative treatment available to date.. The HLA class I allele HLAC*06:02 is the main genetic risk determinant of psoriasis.



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    a, Representative flow cytometry plot of CD11b expression on OT-I CD8 + T cells 10 days after in vitro stimulation. Representative of four independent experiments. b, Experimental schematic (left) and real-time cytotoxicity assay (xCELLigence; right) evaluating B16-OVA tumor cell lysis when cultured alone or treated OT-I CD11b⁻ or OT-I CD11b⁺ CD8 + T cells (E:T = 0.25:1). Representative of three independent experiments; replicate-level data are shown in  . c, Experimental workflow for generating CD11b-deficient NY-ESO-1-specific human CD8⁺ T cells. Cells were transduced with an NY-ESO-1-specific TCR, selected for CD11b expression, edited using CRISPR–Cas9, expanded and assessed for antitumor activity in real-time cytotoxicity assays. d, Representative flow cytometry plots showing CD11b expression in control-electroporated (CD11b + ctrl) and ITGAM-targeted (CD11b KO) NY-ESO-1-specific CD8 + T cells (left), and real-time cytotoxicity assay (xCELLigence; right) evaluating SK-MEL28-NY-ESO-1 tumor cell lysis when cultured alone, or with CD11b + ctrl or CD11b KO NY-ESO-1 CD8 + T cells (E:T = 0.25:1). Representative of three independent experiments; CD11b knockout efficiency and two additional independent cytotoxicity experiments are shown in  and  , respectively. e, Representative flow cytometry plot (left) and quantification (right) of CD11b expression in untransduced (UTD) or 19BBz cells after 10 days of in vitro expansion (n = 7 donors). f, Real-time cytotoxicity assay (xCELLigence) evaluating SK-MEL28-CD19t tumor cell lysis when cultured alone or with CD11b + or CD11b⁻ 19BBz CAR-T cells (E:T = 0.25:1). Representative of three independent experiments; replicate-level data are shown in  . P values were calculated using two-tailed, paired Student’s t test ( e ).

    Journal: bioRxiv

    Article Title: Noncanonical CD11b on CD8 + T cells promotes cytotoxic effector function

    doi: 10.64898/2026.10.07.757268

    Figure Lengend Snippet: a, Representative flow cytometry plot of CD11b expression on OT-I CD8 + T cells 10 days after in vitro stimulation. Representative of four independent experiments. b, Experimental schematic (left) and real-time cytotoxicity assay (xCELLigence; right) evaluating B16-OVA tumor cell lysis when cultured alone or treated OT-I CD11b⁻ or OT-I CD11b⁺ CD8 + T cells (E:T = 0.25:1). Representative of three independent experiments; replicate-level data are shown in . c, Experimental workflow for generating CD11b-deficient NY-ESO-1-specific human CD8⁺ T cells. Cells were transduced with an NY-ESO-1-specific TCR, selected for CD11b expression, edited using CRISPR–Cas9, expanded and assessed for antitumor activity in real-time cytotoxicity assays. d, Representative flow cytometry plots showing CD11b expression in control-electroporated (CD11b + ctrl) and ITGAM-targeted (CD11b KO) NY-ESO-1-specific CD8 + T cells (left), and real-time cytotoxicity assay (xCELLigence; right) evaluating SK-MEL28-NY-ESO-1 tumor cell lysis when cultured alone, or with CD11b + ctrl or CD11b KO NY-ESO-1 CD8 + T cells (E:T = 0.25:1). Representative of three independent experiments; CD11b knockout efficiency and two additional independent cytotoxicity experiments are shown in and , respectively. e, Representative flow cytometry plot (left) and quantification (right) of CD11b expression in untransduced (UTD) or 19BBz cells after 10 days of in vitro expansion (n = 7 donors). f, Real-time cytotoxicity assay (xCELLigence) evaluating SK-MEL28-CD19t tumor cell lysis when cultured alone or with CD11b + or CD11b⁻ 19BBz CAR-T cells (E:T = 0.25:1). Representative of three independent experiments; replicate-level data are shown in . P values were calculated using two-tailed, paired Student’s t test ( e ).

    Article Snippet: Unless otherwise stated, T cells were activated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific, #11132D) at a bead-to-cell ratio of 1:1 in CST OpTimizer T Cell Expansion medium (Thermo Fisher Scientific, #A1048501) containing 5% CTS Immune Cell Serum Replacement (Thermo Fisher Scientific, #A2596101) and recombinant human IL-2 (100 IU/mL; Miltenyi Biotec, #130-097-748).

    Techniques: Flow Cytometry, Expressing, In Vitro, Cytotoxicity Assay, Lysis, Cell Culture, Transduction, CRISPR, Activity Assay, Control, Knock-Out, Two Tailed Test, FACS