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preabsorption tests hcn4 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs preabsorption tests hcn4 antibody
    Preabsorption Tests Hcn4 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/preabsorption/Anti-HCN4+Antibody/pm23853005-148-1-13
    Average 96 stars, based on 195 article reviews
    preabsorption tests hcn4 antibody - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Potential roles of β3-adrenergic receptor in heart rate regulation via sinus node activity and local adipocyte metabolism.
    Article Snippet: .. The sections were then incubated at 4 ◦C overnight with either anti-mouse HCN4 antibody (Alomone Lab, APC052, 1:100 dilution) or β3-AR antibody (Alomone Lab, AAR-017, 1:50 dilution). ..

    Cell-Signaling:

    Article Title: The HCN4 channel modulates external tufted cell burst firing.
    Article Snippet: Membrane blots were incubated in TBST buffer with 5% 168 powder milk for 1 h, and then probed in 4 °C overnight with one of the following primary 169 antibodies in same buffer: rabbit anti-HCN1 (#APC-056, 1:200), -HCN2 (#APC-030, 1:200), -170 journals.physiology.org/journal/jn (123.031.253.151) on August 20, 2026 .. HCN3 (#APC-057, 1:200) and -HCN4 (#APC-052, 1:200) antibody (Alomone Labs, Jerusalem, 171 Israel) and rabbit anti-β-actin (1:2500; Cell Signaling Technology, Danvers, MA, USA). .. Wet blots 172 were incubated in secondary HRP-linked anti-rabbit IgG (Cell Signaling Technology, Danvers, 173 MA, USA) in 5% milk for 1 h and visualized using Pierce Biotechnology ECL Western blotting 174 substrate kit (Thermo Fisher, Waltham, MA, USA).

    Article Title: 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3D
    Article Snippet: SBRs were calculated as has been reported, , , , , , by detecting the mean signal intensity in an ROI within the stained structure, and dividing this by the averaged mean signal intensity obtained from three adjacent background regions using the same ROI (ImageJ ROI function). .. Antibodies used in this study were: anti-α-smooth muscle actin (F3777 clone 1A4, Sigma, for titration studies standard concentration of 1:200 of 1mg/ml stock concentration; for comparative studies of 3D imaging protocols, at 1:1000), anti-ENAC (Ergonul et al., at 1:200), anti-PARVALBUMIN (R&D AF5058, at 1:200), anti-ENDOMUCIN (ab106100, Abcam, at 1:200), anti-PODOCALYXIN (3169, R&D Systems, at 1:200), anti-HCN4 (APC-052, Alomone, at 1:500), anti-PDGFR-β (3169, Cell Signaling Technology, at 1:100), anti-CD31 (BD Biosciences, 550389 at 1/500), anti-E-CADHERIN (AF748, R&D, for titration studies standard concentration of 1:200 of .2mg/ml stock), anti-MCAM (134702, BioLegend, for titration studies standard concentration of 1:500 of .5mg/ml stock), and anti-cardiac TROPONIN-T (NB110–2546, R&D Systems, at 1:500), anti-GFRα1 (AF560, R&D Systems at 1:200), anti-STRA8 (Ab49405, Abcam at 1:200), and anti-cKIT (AF1356, R&D Systems at 1:200). ..

    Negative Control:

    Article Title: Hcn4-deficient zebrafish embryos develop a normal left‒right axis
    Article Snippet: .. The primary antibody used to label Hcn4 was a rabbit polyclonal anti-HCN4 antibody (Alomone labs APC-052) at a ratio of 1:100; a negative control was also included (no primary antibody). .. The next day, several washes with PBS-TD were performed, and the embryos were subsequently incubated overnight at 4 °C in PBS-TD with the Alexa Fluor 555 F(ab’)2-Goat anti-rabbit IgG (H + L) secondary antibody (Thermo Fisher Scientific) at a ratio of 1:200.

    other:

    Article Title: HCN4 gain-of-function mutation increases intrinsic heart rate and limits maladaptive remodeling under pressure overload
    Article Snippet: Images were recorded using a Leica SP8 confocal microscope and analyzed using ImageJ ( ) to quantify fluorescence intensity ( ).

    Article Title: 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3D
    Article Snippet: Anti-HCN4, rabbit polyclonal , Alomone Labs , Cat# APC-052; RRID: AB_2039906.

    Titration:

    Article Title: 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3D
    Article Snippet: SBRs were calculated as has been reported, , , , , , by detecting the mean signal intensity in an ROI within the stained structure, and dividing this by the averaged mean signal intensity obtained from three adjacent background regions using the same ROI (ImageJ ROI function). .. Antibodies used in this study were: anti-α-smooth muscle actin (F3777 clone 1A4, Sigma, for titration studies standard concentration of 1:200 of 1mg/ml stock concentration; for comparative studies of 3D imaging protocols, at 1:1000), anti-ENAC (Ergonul et al., at 1:200), anti-PARVALBUMIN (R&D AF5058, at 1:200), anti-ENDOMUCIN (ab106100, Abcam, at 1:200), anti-PODOCALYXIN (3169, R&D Systems, at 1:200), anti-HCN4 (APC-052, Alomone, at 1:500), anti-PDGFR-β (3169, Cell Signaling Technology, at 1:100), anti-CD31 (BD Biosciences, 550389 at 1/500), anti-E-CADHERIN (AF748, R&D, for titration studies standard concentration of 1:200 of .2mg/ml stock), anti-MCAM (134702, BioLegend, for titration studies standard concentration of 1:500 of .5mg/ml stock), and anti-cardiac TROPONIN-T (NB110–2546, R&D Systems, at 1:500), anti-GFRα1 (AF560, R&D Systems at 1:200), anti-STRA8 (Ab49405, Abcam at 1:200), and anti-cKIT (AF1356, R&D Systems at 1:200). ..

    Concentration Assay:

    Article Title: 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3D
    Article Snippet: SBRs were calculated as has been reported, , , , , , by detecting the mean signal intensity in an ROI within the stained structure, and dividing this by the averaged mean signal intensity obtained from three adjacent background regions using the same ROI (ImageJ ROI function). .. Antibodies used in this study were: anti-α-smooth muscle actin (F3777 clone 1A4, Sigma, for titration studies standard concentration of 1:200 of 1mg/ml stock concentration; for comparative studies of 3D imaging protocols, at 1:1000), anti-ENAC (Ergonul et al., at 1:200), anti-PARVALBUMIN (R&D AF5058, at 1:200), anti-ENDOMUCIN (ab106100, Abcam, at 1:200), anti-PODOCALYXIN (3169, R&D Systems, at 1:200), anti-HCN4 (APC-052, Alomone, at 1:500), anti-PDGFR-β (3169, Cell Signaling Technology, at 1:100), anti-CD31 (BD Biosciences, 550389 at 1/500), anti-E-CADHERIN (AF748, R&D, for titration studies standard concentration of 1:200 of .2mg/ml stock), anti-MCAM (134702, BioLegend, for titration studies standard concentration of 1:500 of .5mg/ml stock), and anti-cardiac TROPONIN-T (NB110–2546, R&D Systems, at 1:500), anti-GFRα1 (AF560, R&D Systems at 1:200), anti-STRA8 (Ab49405, Abcam at 1:200), and anti-cKIT (AF1356, R&D Systems at 1:200). ..

    3D Imaging:

    Article Title: 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3D
    Article Snippet: SBRs were calculated as has been reported, , , , , , by detecting the mean signal intensity in an ROI within the stained structure, and dividing this by the averaged mean signal intensity obtained from three adjacent background regions using the same ROI (ImageJ ROI function). .. Antibodies used in this study were: anti-α-smooth muscle actin (F3777 clone 1A4, Sigma, for titration studies standard concentration of 1:200 of 1mg/ml stock concentration; for comparative studies of 3D imaging protocols, at 1:1000), anti-ENAC (Ergonul et al., at 1:200), anti-PARVALBUMIN (R&D AF5058, at 1:200), anti-ENDOMUCIN (ab106100, Abcam, at 1:200), anti-PODOCALYXIN (3169, R&D Systems, at 1:200), anti-HCN4 (APC-052, Alomone, at 1:500), anti-PDGFR-β (3169, Cell Signaling Technology, at 1:100), anti-CD31 (BD Biosciences, 550389 at 1/500), anti-E-CADHERIN (AF748, R&D, for titration studies standard concentration of 1:200 of .2mg/ml stock), anti-MCAM (134702, BioLegend, for titration studies standard concentration of 1:500 of .5mg/ml stock), and anti-cardiac TROPONIN-T (NB110–2546, R&D Systems, at 1:500), anti-GFRα1 (AF560, R&D Systems at 1:200), anti-STRA8 (Ab49405, Abcam at 1:200), and anti-cKIT (AF1356, R&D Systems at 1:200). ..



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