total pre rna amplification kit (Thermo Fisher)
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Thermo Fisher
total pre rna amplification kit
Total Pre Rna Amplification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 496 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pre-amplification+kit/Total+Exosome+RNA+%26+Protein+Isolation+Kit/pmc03759409-65-27-32
Average 97 stars, based on 496 article reviews
Total Pre Rna Amplification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 496 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pre-amplification+kit/Total+Exosome+RNA+%26+Protein+Isolation+Kit/pmc03759409-65-27-32
Average 97 stars, based on 496 article reviews
total pre rna amplification kit - by Bioz Stars,
2026-11
97/100 stars
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Isolation:Article Title: Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes Article Snippet: .. For the integrity analysis, the RNA from breast cancer cells and exosomes was extracted using the total exosome RNA and the Article Title: N -ethylmaleimide-sensitive factor interacts with the serotonin transporter and modulates its trafficking: implications for pathophysiology in autism Article Snippet: Cells were incubated with MTT solution at 37 oC for 6 h. Following removal of the solution, dimethyl sulfoxide was added, and the amount of formazan formed was measured spectrophotometrically at 550 nm using a microplate reader (Bio-Rad, Hercules, CA, USA). .. Biotinylation experiments were performed using the Article Title: m 6 A Demethylase FTO Stabilizes LINK-A to Exert Oncogenic Roles via MCM3-Mediated Cell Cycle Progression and HIF-1α Activation Article Snippet: .. Chromatin-binding proteins were isolated from cultured cells using a Biotinylation:Article Title: N -ethylmaleimide-sensitive factor interacts with the serotonin transporter and modulates its trafficking: implications for pathophysiology in autism Article Snippet: Cells were incubated with MTT solution at 37 oC for 6 h. Following removal of the solution, dimethyl sulfoxide was added, and the amount of formazan formed was measured spectrophotometrically at 550 nm using a microplate reader (Bio-Rad, Hercules, CA, USA). .. Biotinylation experiments were performed using the Microarray:Article Title: A new type of transcriptional reprogramming by an IRF4 mutation in lymphoma Article Snippet: .. For microarray gene expression analyses of BJAB cells following tet-induction of IRF4-WT or IRF4-C99R, mRNA was processed by use of the Illumina Gene Expression:Article Title: A new type of transcriptional reprogramming by an IRF4 mutation in lymphoma Article Snippet: .. For microarray gene expression analyses of BJAB cells following tet-induction of IRF4-WT or IRF4-C99R, mRNA was processed by use of the Illumina RNA Amplification:Article Title: A new type of transcriptional reprogramming by an IRF4 mutation in lymphoma Article Snippet: .. For microarray gene expression analyses of BJAB cells following tet-induction of IRF4-WT or IRF4-C99R, mRNA was processed by use of the Illumina Article Title: The dynamics of E1A in regulating networks and canonical pathways in quiescent cells Article Snippet: The microarray analysis was performed at the Cleveland Clinic Genomics core facility by using Agilent Mouse Whole Genome Arrays (Agilent, Santa Clara, CA) in a balanced block design. .. Total RNA (250 ng) was reverse transcribed into cRNA and biotin-UTP labeled using the Illumina HAT Assay:Article Title: A new type of transcriptional reprogramming by an IRF4 mutation in lymphoma Article Snippet: .. For microarray gene expression analyses of BJAB cells following tet-induction of IRF4-WT or IRF4-C99R, mRNA was processed by use of the Illumina Cell Culture:Article Title: m 6 A Demethylase FTO Stabilizes LINK-A to Exert Oncogenic Roles via MCM3-Mediated Cell Cycle Progression and HIF-1α Activation Article Snippet: .. Chromatin-binding proteins were isolated from cultured cells using a Reverse Transcription:Article Title: The dynamics of E1A in regulating networks and canonical pathways in quiescent cells Article Snippet: The microarray analysis was performed at the Cleveland Clinic Genomics core facility by using Agilent Mouse Whole Genome Arrays (Agilent, Santa Clara, CA) in a balanced block design. .. Total RNA (250 ng) was reverse transcribed into cRNA and biotin-UTP labeled using the Illumina Labeling:Article Title: The dynamics of E1A in regulating networks and canonical pathways in quiescent cells Article Snippet: The microarray analysis was performed at the Cleveland Clinic Genomics core facility by using Agilent Mouse Whole Genome Arrays (Agilent, Santa Clara, CA) in a balanced block design. .. Total RNA (250 ng) was reverse transcribed into cRNA and biotin-UTP labeled using the Illumina Staining:Article Title: Basal cell adhesion molecule promotes metastasis‐associated processes in ovarian cancer Article Snippet: For detection of soluble BCAM, confluent cells were cultured in serum‐free medium for 24 h. Conditioned media were collected and concentrated 20‐fold using Vivaspin® 6 centrifugal concentrators (#512‐3777; Sartorius/VWR; Göttingen, Germany) and equal amounts of proteins were mixed with SDS‐PAGE sample buffer and analysed according to standard western blotting protocols. .. Pierce Western Blot:Article Title: Basal cell adhesion molecule promotes metastasis‐associated processes in ovarian cancer Article Snippet: For detection of soluble BCAM, confluent cells were cultured in serum‐free medium for 24 h. Conditioned media were collected and concentrated 20‐fold using Vivaspin® 6 centrifugal concentrators (#512‐3777; Sartorius/VWR; Göttingen, Germany) and equal amounts of proteins were mixed with SDS‐PAGE sample buffer and analysed according to standard western blotting protocols. .. Pierce Protein Extraction:Article Title: Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis Article Snippet: .. The total protein from treated cells was extracted by the Total other:Article Title: Practical tips and new trends in electrochemical biosensing of cancer-related extracellular vesicles Article Snippet: |
![(A) A representative Northern blot identified canonical myostatin (Mstn) and MSV mRNAs in poly(A) <t>+</t> <t>RNA</t> isolated from sheep skeletal muscle using a radiolabeled probe complementary to exon 1 & 2 sequence of sheep myostatin (nt 1–621). (B) Schematic representation of alternative splicing of the myostatin gene. Genomic structure, splicing of canonical myostatin and MSV mRNAs are shown as determined by RT-PCR amplification and sequencing. The sheep myostatin gene has a cryptic third intron sequence (Int 3, 1011 bp) located 21 bp downstream of the intron 2/exon 3 boundary, thereby removing the coding sequence of the canonical mature myostatin protein. Alternate splicing creates a new ORF (966 bp) by appending a novel C-terminal coding sequence (exon 3b, 198 bp) to a truncated propeptide coding sequence of myostatin (exon 1 & 2 and 3a) in the MSV transcript. Open boxes show 5′ and 3′ untranslated regions, filled boxes represent translated sequences. Also shown are exons (Ex), introns (Int), translation start (ATG) and stop (TGA, TAA) sites, and the size of each transcript. Location of the 11 bp deletion in exon 3 identified in Belgian Blue cattle is also indicated. (C) Tissue-specific mRNA expression of MSV and myostatin was analyzed in biceps femoris (Biceps), quadriceps (Quad.) and semitendinosus (Semit.) muscles, and heart, liver, brain, kidney, testicle, ovary, gut, skin and aorta tissues of <t>three</t> months old sheep using RT-PCR. Actin was used as a positive control for each tissue sample. NTC is a no template PCR control. (D) Multiple polypeptide sequence alignment of the predicted C-terminus of MSV in sheep, cattle, pig and dolphin. A consensus proteolytic cleavage site [(K/R)-(X) n -(K/R)↓ where n = 0, 2, 4, 6 and X is any amino acid except cysteine at aa 271–274] has been identified for precursor convertases. A dotted line indicates the location of the putative cleavage site. The scale shows the positions of the amino acid residues in the MSV polypeptide sequence. The unshaded background highlights residues that are different from the consensus sequence. An in-silico predicted secondary structure of mature sheep MSV is also shown. (E) Schematic representation of the known and proposed proteolytic processing of canonical myostatin and MSV precursors, respectively. The location of the secretion signal peptide and the C-terminal cleavage sites are indicated. Grey filling shows the novel C-terminus of the MSV precursor. Black bars denote the location of polypeptide sequences used to raise MSV-specific polyclonal antibodies (MSVab and MSVab65). (F) Detection of MSV-immunoreactive proteins in semitendinosus muscles of sheep and cattle and its absence in gastrocnemius muscles of mouse and rat (20 µg of total protein per lane) using an anti-MSVab in Western immunoblotting. Recombinant peptide (Recomb.) corresponds to a polypeptide for the C-terminal 65 amino acids (11.9 kDa) of sheep MSV. Molecular weights of a protein marker are also indicated.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6833/pmc03846833/pmc03846833__pone.0081713.g001.jpg)