expression vector ppiczac (Thermo Fisher)
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expression vector ppiczac
Expression Vector Ppiczac, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppiczac+vector/pm23526366-48-24-27
Average 86 stars, based on 1 article reviews
Expression Vector Ppiczac, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppiczac+vector/pm23526366-48-24-27
Average 86 stars, based on 1 article reviews
expression vector ppiczac - by Bioz Stars,
2026-11
86/100 stars
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Expressing:Article Title: Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris. Article Snippet: Gene synthesis and sub-cloning of ttcbh6B Codons in the ORF of ttcbh6B (NCBI gene ID: 11521362) were optimized for expression in P. pastoris and synthesized by Invitrogen/Life Technologies. .. ClaI and XbaI were added to the 50- and 30-ends respectively and used to insert a sub-clone of ttcbh6B into the Article Title: Cloning and optimized expression of a GH-11 xylanase from Fusarium oxysporum in Pichia pastoris. Article Snippet: The endo-1,4-b-xylanase gene xyn11a from Fusarium oxysporum, member of the fungal glycosyl hydrolase (GH) family 11, was cloned and expressed in Pichia pastoris.. The mature xylanase gene, which generates after the excision of one intron and the secreting signal peptide, was placed under the control of an alcohol oxidase promoter (AOX1) in the plasmid pPICZaC.. The final construction was integrated into the genome of the methylotrophic yeast P. pastoris X33 and the ability to produce xylanase activity was evaluated in flask cultures. Article Title: Ornithodoros savignyi: soft tick apyrase belongs to the 5'-nucleotidase family. Article Snippet: Salivary apyrases are nucleotide-metabolising enzymes that blood-feeding parasites utilise for modulation of extracellular nucleotides to prevent platelet activation and aggregation.. In this study a 50-nucleotidase specific degenerate primer was used to identify homologous transcripts from Ornithodoros savignyi salivary gland cDNA.. Two 50-nucleotidase isoforms that share significant sequence identity to putative apyrases from Rhipicephalus appendiculatus and Ixodes scapularis were identified. Article Title: His22 of TLXI plays a critical role in the inhibition of glycoside hydrolase family 11 xylanases. Article Snippet: Prior to cloning in a pCRw4-TOPOw vector, PCR products were gel extracted (QIAquick Gel Extraction Kit, Qiagen) and 30deoxyadenylate-overhangs were added with SuperTaq polymerase (SphaeroQ, Leiden, The Netherlands) (2.5 U, 728C, 10 min). .. After sequence verification, the tlxi gene containing the desired mutation was subcloned as a Bgl II fragment in the Bsm BI site of a Article Title: Secreted recombinant P53 protein from Pichia pastoris is a useful antigen for detection of serum p53: autoantibody in patients with advanced colorectal adenocarcinoma. Article Snippet: The detection of P53 alteration by serological method is easier to perform, does not require tumor tissues and is of interest for patients monitoring.. In this study, we described the development of a home made ELISA test based on recombinant human P53 protein produced in Pichia pastoris and used as antigen for the detection of serum p53-Abs in colorectal carcinoma patients.. The human P53 was secreted as a His-tagged protein by recombinant KM71 strain (Ka21) via the peptide signal a of the Saccharomyces cerevisiae mating type gene. Plasmid Preparation:Article Title: Cloning and optimized expression of a GH-11 xylanase from Fusarium oxysporum in Pichia pastoris. Article Snippet: The endo-1,4-b-xylanase gene xyn11a from Fusarium oxysporum, member of the fungal glycosyl hydrolase (GH) family 11, was cloned and expressed in Pichia pastoris.. The mature xylanase gene, which generates after the excision of one intron and the secreting signal peptide, was placed under the control of an alcohol oxidase promoter (AOX1) in the plasmid pPICZaC.. The final construction was integrated into the genome of the methylotrophic yeast P. pastoris X33 and the ability to produce xylanase activity was evaluated in flask cultures. Article Title: Distinct catalytic capacities of two aluminium-repressed Arabidopsis thaliana xyloglucan endotransglucosylase/hydrolases, XTH15 and XTH31, heterologously produced in Pichia. Article Snippet: Xyloglucan plays an important structural role in primary cell walls, possibly tethering adjacent microfibrils and restraining cell expansion.. There is therefore considerable interest in understanding the role of xyloglucan endotransglucosylase/hydrolases (XTHs), which are encoded in Arabidopsis by a 33-member gene family.. We compared the key catalytic properties of two very different Arabidopsis XTHs (heterologously produced in Pichia), both of which are aluminium-repressed. Article Title: His22 of TLXI plays a critical role in the inhibition of glycoside hydrolase family 11 xylanases. Article Snippet: Prior to cloning in a pCRw4-TOPOw vector, PCR products were gel extracted (QIAquick Gel Extraction Kit, Qiagen) and 30deoxyadenylate-overhangs were added with SuperTaq polymerase (SphaeroQ, Leiden, The Netherlands) (2.5 U, 728C, 10 min). .. After sequence verification, the tlxi gene containing the desired mutation was subcloned as a Bgl II fragment in the Bsm BI site of a Article Title: Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor Article Snippet: The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM).. We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM.. The binding affinity of NCAM–FGFR interactions was considerably higher for splice variant ‘b’ than for splice variant ‘c’. Article Title: Secreted recombinant P53 protein from Pichia pastoris is a useful antigen for detection of serum p53: autoantibody in patients with advanced colorectal adenocarcinoma. Article Snippet: The detection of P53 alteration by serological method is easier to perform, does not require tumor tissues and is of interest for patients monitoring.. In this study, we described the development of a home made ELISA test based on recombinant human P53 protein produced in Pichia pastoris and used as antigen for the detection of serum p53-Abs in colorectal carcinoma patients.. The human P53 was secreted as a His-tagged protein by recombinant KM71 strain (Ka21) via the peptide signal a of the Saccharomyces cerevisiae mating type gene. Article Title: Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris Article Snippet: Antifreeze proteins (AFPs) are unique proteins that have an affinity toward ice.. Due to their potential applications in the food and medical industries, many attempts have been made to produce AFPs in large quantities.. In this study, recombinant Afp1 from Glaciozyma antarctica, a psychrophilic yeast, was overexpressed in a methylotrophic yeast,Pichia pastoris, followed by 5-L fermenter expression. Sequencing:Article Title: Distinct catalytic capacities of two aluminium-repressed Arabidopsis thaliana xyloglucan endotransglucosylase/hydrolases, XTH15 and XTH31, heterologously produced in Pichia. Article Snippet: Xyloglucan plays an important structural role in primary cell walls, possibly tethering adjacent microfibrils and restraining cell expansion.. There is therefore considerable interest in understanding the role of xyloglucan endotransglucosylase/hydrolases (XTHs), which are encoded in Arabidopsis by a 33-member gene family.. We compared the key catalytic properties of two very different Arabidopsis XTHs (heterologously produced in Pichia), both of which are aluminium-repressed. Article Title: His22 of TLXI plays a critical role in the inhibition of glycoside hydrolase family 11 xylanases. Article Snippet: Prior to cloning in a pCRw4-TOPOw vector, PCR products were gel extracted (QIAquick Gel Extraction Kit, Qiagen) and 30deoxyadenylate-overhangs were added with SuperTaq polymerase (SphaeroQ, Leiden, The Netherlands) (2.5 U, 728C, 10 min). .. After sequence verification, the tlxi gene containing the desired mutation was subcloned as a Bgl II fragment in the Bsm BI site of a Construct:Article Title: Ornithodoros savignyi: soft tick apyrase belongs to the 5'-nucleotidase family. Article Snippet: Salivary apyrases are nucleotide-metabolising enzymes that blood-feeding parasites utilise for modulation of extracellular nucleotides to prevent platelet activation and aggregation.. In this study a 50-nucleotidase specific degenerate primer was used to identify homologous transcripts from Ornithodoros savignyi salivary gland cDNA.. Two 50-nucleotidase isoforms that share significant sequence identity to putative apyrases from Rhipicephalus appendiculatus and Ixodes scapularis were identified. Mutagenesis:Article Title: His22 of TLXI plays a critical role in the inhibition of glycoside hydrolase family 11 xylanases. Article Snippet: Prior to cloning in a pCRw4-TOPOw vector, PCR products were gel extracted (QIAquick Gel Extraction Kit, Qiagen) and 30deoxyadenylate-overhangs were added with SuperTaq polymerase (SphaeroQ, Leiden, The Netherlands) (2.5 U, 728C, 10 min). .. After sequence verification, the tlxi gene containing the desired mutation was subcloned as a Bgl II fragment in the Bsm BI site of a Amplification:Article Title: Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor Article Snippet: The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM).. We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM.. The binding affinity of NCAM–FGFR interactions was considerably higher for splice variant ‘b’ than for splice variant ‘c’. Clone Assay:Article Title: Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor Article Snippet: The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM).. We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM.. The binding affinity of NCAM–FGFR interactions was considerably higher for splice variant ‘b’ than for splice variant ‘c’. Article Title: Secreted recombinant P53 protein from Pichia pastoris is a useful antigen for detection of serum p53: autoantibody in patients with advanced colorectal adenocarcinoma. Article Snippet: The detection of P53 alteration by serological method is easier to perform, does not require tumor tissues and is of interest for patients monitoring.. In this study, we described the development of a home made ELISA test based on recombinant human P53 protein produced in Pichia pastoris and used as antigen for the detection of serum p53-Abs in colorectal carcinoma patients.. The human P53 was secreted as a His-tagged protein by recombinant KM71 strain (Ka21) via the peptide signal a of the Saccharomyces cerevisiae mating type gene. Article Title: Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris Article Snippet: Antifreeze proteins (AFPs) are unique proteins that have an affinity toward ice.. Due to their potential applications in the food and medical industries, many attempts have been made to produce AFPs in large quantities.. In this study, recombinant Afp1 from Glaciozyma antarctica, a psychrophilic yeast, was overexpressed in a methylotrophic yeast,Pichia pastoris, followed by 5-L fermenter expression. Polymerase Chain Reaction:Article Title: Secreted recombinant P53 protein from Pichia pastoris is a useful antigen for detection of serum p53: autoantibody in patients with advanced colorectal adenocarcinoma. Article Snippet: The detection of P53 alteration by serological method is easier to perform, does not require tumor tissues and is of interest for patients monitoring.. In this study, we described the development of a home made ELISA test based on recombinant human P53 protein produced in Pichia pastoris and used as antigen for the detection of serum p53-Abs in colorectal carcinoma patients.. The human P53 was secreted as a His-tagged protein by recombinant KM71 strain (Ka21) via the peptide signal a of the Saccharomyces cerevisiae mating type gene. Purification:Article Title: Secreted recombinant P53 protein from Pichia pastoris is a useful antigen for detection of serum p53: autoantibody in patients with advanced colorectal adenocarcinoma. Article Snippet: The detection of P53 alteration by serological method is easier to perform, does not require tumor tissues and is of interest for patients monitoring.. In this study, we described the development of a home made ELISA test based on recombinant human P53 protein produced in Pichia pastoris and used as antigen for the detection of serum p53-Abs in colorectal carcinoma patients.. The human P53 was secreted as a His-tagged protein by recombinant KM71 strain (Ka21) via the peptide signal a of the Saccharomyces cerevisiae mating type gene. Recombinant:Article Title: Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris Article Snippet: Antifreeze proteins (AFPs) are unique proteins that have an affinity toward ice.. Due to their potential applications in the food and medical industries, many attempts have been made to produce AFPs in large quantities.. In this study, recombinant Afp1 from Glaciozyma antarctica, a psychrophilic yeast, was overexpressed in a methylotrophic yeast,Pichia pastoris, followed by 5-L fermenter expression. Transformation Assay:Article Title: Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris Article Snippet: Antifreeze proteins (AFPs) are unique proteins that have an affinity toward ice.. Due to their potential applications in the food and medical industries, many attempts have been made to produce AFPs in large quantities.. In this study, recombinant Afp1 from Glaciozyma antarctica, a psychrophilic yeast, was overexpressed in a methylotrophic yeast,Pichia pastoris, followed by 5-L fermenter expression. |