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pp2a a:c dimer  (Millipore)


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    Structured Review

    Millipore pp2a a:c dimer
    Pp2a A:C Dimer, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pp2a+dimer/pp2a+immunoprecipitation+phosphatase+assay+kit/pmc03526344-679-6-9
    Average 90 stars, based on 1 article reviews
    pp2a a:c dimer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: SET-binding protein 1 (SETBP1) suppresses cell proliferation in estrogen receptor-positive breast cancer.
    Article Snippet: In brief, PP2A-specific reaction buffer (Millipore) containing 750 mM phosphopeptide substrate was added to cell lysates prepared in a low-detergent lysis buffer.

    Article Title: Meiosis-specific distal cohesion site decoupled from the kinetochore
    Article Snippet: The following primary antibodies were used at the indicated delusions for both oocytes and somatic cells: rabbit anti-mouse REC8 (1:200, gift from Michael A. Lampson), mouse anti-human PP2A C subunit (1:100, EMD Millipore, cat# 05-421-AF488), rabbit anti-human Survivin (1:100, Cell Signaling Technology. cat# 2808), rabbit anti-human phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198), pAurora (1:100, Cell Signaling Technology, cat# 2914S), rabbit anti-human MCAK (1:1000, gift from Duane Compton), rabbit anti-histone H3-pT3 (1:100, Active Motif, cat# 39154), sheep polyclonal anti human-BUB1 antibody, SB1.3 (1:50, gift from Stephen Taylor), rabbit anti-histone H2A-pT120 (1:2000, Active motif, cat# 39391), mouse anti-human HEC1 (1:200, Santa Cruz, cat# sc-515550), CREST human autoantibody against centromere, ACA (1:100, Immunovision, cat# HCT-0100), goat anti-GFP antibody conjugated with Dylight488 (1:100, Rockland, cat# 600-141-215).

    Article Title: In vitro studies on stability and phosphatase activity of the anticancer agent LB-100 and its active hydrolysis product endothall.
    Article Snippet: LB-100, a small molecule PP2A inhibitor currently in phase 1 and 2 dose-finding and efficacy trials, is an amide that is stable at pH ≥ 10.5, but can hydrolyze at lower pH values to endothall and Nmethylpiperazine.. Endothall has been detected in plasma of patients after i.v. LB-100 administration, but in vitro LB-100 hydrolysis has not been studied in detail.. LC-MS/MS assays of various LB-100 solutions showed that LB-100 rapidly hydrolyzes in aqueous solutions at room temperature (RT) at pH 5.6 6.5 to endothall (t1/2 = 2.1 3.3 h).

    Article Title: A PP2A-mtATR-tBid axis links DNA damage-induced CIP2A degradation to apoptotic dormancy and therapeutic resistance in PDAC.
    Article Snippet: To verify that the elimination of mtATR by LB-100 occurred through inhibition of PP2A activity, we assessed PP2A activity using an immunoprecipitation-based PP2A phosphatase assay (MilliporeSigma).

    Article Title: Meiosis-specific distal cohesion site decoupled from the kinetochore.
    Article Snippet: The following primary antibodies were used at the indicated delusions for both oocytes and somatic cells: rabbit anti-mouse REC8 (1:200, gift from Michael A. Lampson), mouse anti-human PP2A C subunit (1:100, EMD Millipore, cat# 05-421-AF488), rabbit anti-human Survivin (1:100, Cell Signaling Technology. cat# 2808), rabbit antihuman phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198), pAurora (1:100, Cell Signaling Technology, cat# 2914S), rabbit anti-human MCAK (1:1000, gift from Duane Compton), rabbit anti-histone H3-pT3 (1:100, ActiveMotif, cat# 39154), sheep polyclonal anti human-BUB1 antibody, SB1.3 (1:50, gift from Stephen Taylor), rabbit anti-histone H2A-pT120 (1:2000, Active motif, cat# 39391), mouse anti-human HEC1 (1:200, Santa Cruz, cat# sc-515550), CREST human autoantibody against centromere, ACA (1:100, Immunovision, Nature Communications | (2025) 16:2116 11 cat# HCT-0100), goat anti-GFP antibody conjugated with Dylight488 (1:100, Rockland, cat# 600-141-215).

    Activity Assay:

    Article Title: PP2A activation overcomes leptomeningeal dissemination in group 3 medulloblastoma.
    Article Snippet: .. PP2A activity was measured using a PP2A Immunoprecipitation Phosphatase Assay Kit (17–313, Millipore Sigma) per manufacturer’s protocol. .. Briefly, protein lysates were incubated with PP2A antibody at 4 C with continuous rotation for 2 h. Following the addition of assay buffers and malachite green solution, the plate was read at an absorbance of 650 nm using a microplate reader (Epoch Microplate Spectrophotometer, BioTek Instruments).

    Article Title: Novel PP2A-Activating Compounds in Neuroblastoma
    Article Snippet: .. A PP2A Immunoprecipitation Phosphatase Assay Kit (17–313, EMD Millipore) was utilized per the manufacturer’s protocol to evaluate the activity of PP2A. ..

    Article Title: Tenuigenin inhibits osteosarcoma growth via CIP2A/PP2A/NF-κB axis.
    Article Snippet: Background Polygala tenuifolia and its active components have been revealed to possess anti-tumor activities.. However, the role of Tenuigenin (TEN), a bioactive ingredient from Polygala tenuifolia, in tumors such as osteosarcoma (OS) remains unclear.. The present research intended to explore the efficacy and underlying mechanism of TEN on OS.

    IP Phosphatase Assay:

    Article Title: PP2A activation overcomes leptomeningeal dissemination in group 3 medulloblastoma.
    Article Snippet: .. PP2A activity was measured using a PP2A Immunoprecipitation Phosphatase Assay Kit (17–313, Millipore Sigma) per manufacturer’s protocol. .. Briefly, protein lysates were incubated with PP2A antibody at 4 C with continuous rotation for 2 h. Following the addition of assay buffers and malachite green solution, the plate was read at an absorbance of 650 nm using a microplate reader (Epoch Microplate Spectrophotometer, BioTek Instruments).

    Article Title: Novel PP2A-Activating Compounds in Neuroblastoma
    Article Snippet: .. A PP2A Immunoprecipitation Phosphatase Assay Kit (17–313, EMD Millipore) was utilized per the manufacturer’s protocol to evaluate the activity of PP2A. ..

    Article Title: Tenuigenin inhibits osteosarcoma growth via CIP2A/PP2A/NF-κB axis.
    Article Snippet: Background Polygala tenuifolia and its active components have been revealed to possess anti-tumor activities.. However, the role of Tenuigenin (TEN), a bioactive ingredient from Polygala tenuifolia, in tumors such as osteosarcoma (OS) remains unclear.. The present research intended to explore the efficacy and underlying mechanism of TEN on OS.



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