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Structured Review

Promega powerplex fusion system dc2402
Reagents and resources
Powerplex Fusion System Dc2402, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/powerplex+fusion+system+kit/powerplex++fusion+system+kit/pmc11166601-44-6-4
Average 90 stars, based on 1 article reviews
powerplex fusion system dc2402 - by Bioz Stars, 2026-11
90/100 stars

Images

1) Product Images from "Sonic Hedgehog activates prostaglandin signaling to stabilize primary cilium length"

Article Title: Sonic Hedgehog activates prostaglandin signaling to stabilize primary cilium length

Journal: The Journal of Cell Biology

doi: 10.1083/jcb.202306002

Reagents and resources
Figure Legend Snippet: Reagents and resources

Techniques Used: Recombinant, Bicinchoninic Acid Protein Assay, Reverse Transcription, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Software

Related Articles

other:

Article Title: In silico enhancer mining reveals SNS-032 and EHMT2 inhibitors as therapeutic candidates in high-grade serous ovarian cancer.
Article Snippet: All cell lines used within this study were authenticated at NorthGeneTM using the PowerPlex® Fusion kit (DC24, Promega; WI, USA) in 2022.

Article Title: Rapid and robust validation of pooled CRISPR knockout screens using CelFi.
Article Snippet: All cells were tested and monitored for mycoplasma using the MycoAlertPlusTM Mycoplasma Detection Kit (Lonza, LT07-710) and were authenticated using the PowerPlex® Fusion System (Promega #DC2402) performed at the Hartwell Center (St. Jude Children’s Research Hospital).

Amplification:

Article Title: Comparison of Three DNA Collection Methods on Nile Red Fluorescent Areas of a Porous Substrate
Article Snippet: DNA quantification was performed using an Alu-based realtime PCR method as previously described by Nicklas and Buel21 using a Corbett Rotor-Gene 6000 (Qiagen). .. All samples and controls were amplified using the PowerPlex Fusion System (DC2402; Promega, Madison, WI, USA) on a Veriti 96-Well Thermal Cycler (4375786; ThermoFisher Scientific, Waltham MA, USA) using manufacturer’s recommendations.22 Fragment analysis was performed on an Applied Biosystems 3130xl Genetic Analyzer (ThermoFisher Scientific) and genotyped using GeneMapper ID-X software (version 1.5, A27884; ThermoFisher Scientific). .. The analytical threshold was set at 50 relative fluorescence units (RFU) based on internal validation.

Article Title: Comparison of Three DNA Collection Methods on Nile Red Fluorescent Areas of a Porous Substrate
Article Snippet: DNA quantification was performed using an Alu-based realtime PCR method as previously described by Nicklas and Buel using a Corbett Rotor-Gene 6000 (Qiagen). .. All samples and controls were amplified using the PowerPlex Fusion System (DC2402; Promega, Madison, WI, USA) on a Veriti 96-Well Thermal Cycler (4375786; ThermoFisher Scientific, Waltham MA, USA) using manufacturer’s recommendations. .. Fragment analysis was performed on an Applied Biosystems 3130xl Genetic Analyzer (ThermoFisher Scientific) and genotyped using GeneMapper ID-X software (version 1.5, A27884; ThermoFisher Scientific).

Article Title: Genotyping of Anopheles mosquito blood meals reveals nonrandom human host selection: implications for human-to-mosquito Plasmodium falciparum transmission.
Article Snippet: .. Amplification was performed using a validated, commercially available multiplex PCR kit (Promega, PowerPlex Fusion System; catalog no. DC2402) that contained locus-specific fluorescent-labelled primers [31]. .. PCR products were analysed by capillary electrophoresis (ABI 3730 Genetic Analyzer, Applied Biosystems, Foster City, CA) with LIZ 500 (Applied Biosystems, Foster City, CA) as the internal size standard.

Software:

Article Title: Comparison of Three DNA Collection Methods on Nile Red Fluorescent Areas of a Porous Substrate
Article Snippet: DNA quantification was performed using an Alu-based realtime PCR method as previously described by Nicklas and Buel21 using a Corbett Rotor-Gene 6000 (Qiagen). .. All samples and controls were amplified using the PowerPlex Fusion System (DC2402; Promega, Madison, WI, USA) on a Veriti 96-Well Thermal Cycler (4375786; ThermoFisher Scientific, Waltham MA, USA) using manufacturer’s recommendations.22 Fragment analysis was performed on an Applied Biosystems 3130xl Genetic Analyzer (ThermoFisher Scientific) and genotyped using GeneMapper ID-X software (version 1.5, A27884; ThermoFisher Scientific). .. The analytical threshold was set at 50 relative fluorescence units (RFU) based on internal validation.

Multiplex Assay:

Article Title: Genotyping of Anopheles mosquito blood meals reveals nonrandom human host selection: implications for human-to-mosquito Plasmodium falciparum transmission.
Article Snippet: .. Amplification was performed using a validated, commercially available multiplex PCR kit (Promega, PowerPlex Fusion System; catalog no. DC2402) that contained locus-specific fluorescent-labelled primers [31]. .. PCR products were analysed by capillary electrophoresis (ABI 3730 Genetic Analyzer, Applied Biosystems, Foster City, CA) with LIZ 500 (Applied Biosystems, Foster City, CA) as the internal size standard.

Polymerase Chain Reaction:

Article Title: Genotyping of Anopheles mosquito blood meals reveals nonrandom human host selection: implications for human-to-mosquito Plasmodium falciparum transmission.
Article Snippet: .. Amplification was performed using a validated, commercially available multiplex PCR kit (Promega, PowerPlex Fusion System; catalog no. DC2402) that contained locus-specific fluorescent-labelled primers [31]. .. PCR products were analysed by capillary electrophoresis (ABI 3730 Genetic Analyzer, Applied Biosystems, Foster City, CA) with LIZ 500 (Applied Biosystems, Foster City, CA) as the internal size standard.



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