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port a patch  (Nanion Technologies GmbH)


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    Structured Review

    Nanion Technologies GmbH port a patch
    Port A Patch, supplied by Nanion Technologies GmbH, used in various techniques. Bioz Stars score: 95/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/port-a-patch/Temperature+Control+for+Port-a-Patch/pm32717533-18-7-8
    Average 95 stars, based on 19 article reviews
    port a patch - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Human TRPA1 is an inherently mechanosensitive bilayer-gated ion channel.
    Article Snippet: Purified hTRPA1 and Δ1−688 hTRPA1 were reconstituted into preformed planar lipid bilayers composed of 1,2-diphytanoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids) and cholesterol (Sigma-Aldrich) in a 9:1 ratio and produced by using the Vesicle Prep Pro Station (Nanion Technologies). .. Ion channel activity was recorded using the Port-a-Patch (Nanion Technologies) either by 2-s voltage-ramps (-100 to +100 mV) or at a holding potential of +60 mV in a symmetrical K+ solution (50 mM KCl, 10 mM NaCl, 60 mM KF, 20 mM EGTA, and 10 mM Hepes; adjusted to pH 7.2 with KOH) and at room temperature (20−22 °C). .. Negative pressure was applied in a stepwise manner with the suction control pro (Nanion Technologies) to evoke TRPA1 currents.

    Control:

    Article Title: Delta-type glutamate receptors are ligand-gated ion channels
    Article Snippet: Bound protein was eluted with GDN buffer containing 50 mM biotin (Thermo Scientific, PI29129), collected and concentrated down to 500 μl at 4 °C using a 100 kDa molecular weight cut-off concentrator (Millipore Sigma, UFC9100). .. An Orbit mini with temperature control unit (Nanion Technologies) and MECA 4 recording chips (recording chip with four 100-μm cavities, Nanion Technologies, #132002) were used for all electrophysiological measurements of purified hGluD2 , hGluD2 ΔATD , hGluD2 R710W , and hGluD2 R530K . .. The cleaved protein was further separated via size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (Cytiva, 29091596) equilibrated with the GDN buffer.

    Article Title: Evaluation of Possible Proarrhythmic Potency: Comparison of the Effect of Dofetilide, Cisapride, Sotalol, Terfenadine, and Verapamil on hERG and Native IKr Currents and on Cardiac Action Potential.
    Article Snippet: The proarrhythmic potency of drugs is usually attributed to the IKr current block.. During safety pharmacology testing analysis of IKr in cardiomyocytes was replaced by human ether-a-go-go-related gene (hERG) test using automated patch-clamp systems in stable transfected cell lines.. Aim of this study was to compare the effect of proarrhythmic compounds on hERG and IKr currents and on cardiac action potential.

    Article Title: Delta-type glutamate receptors are ligand-gated ion channels.
    Article Snippet: This is a PDF file of a peer-reviewed paper that has been accepted for publication.. Although unedited, the content has been subjected to preliminary formatting.. Nature is providing this early version of the typeset paper as a service to our authors and readers.

    Article Title: Molecular basis for ligand-gating of the human GluD1 receptor
    Article Snippet: From this, model building, refinement, and structural analysis were carried out using a combination of ChimeraX , ISOLDE , Coot , and PHENIX , as compiled and distributed by the SBGrid Consortium , . .. The electrophysiological measurement of purified GluD1 was done on an Orbit mini plate form with a temperature control unit (Nanion Technologies), using MECA recording chips (4x 100 μm cavities, Nanion Technologies, 132002). ..

    Purification:

    Article Title: Delta-type glutamate receptors are ligand-gated ion channels
    Article Snippet: Bound protein was eluted with GDN buffer containing 50 mM biotin (Thermo Scientific, PI29129), collected and concentrated down to 500 μl at 4 °C using a 100 kDa molecular weight cut-off concentrator (Millipore Sigma, UFC9100). .. An Orbit mini with temperature control unit (Nanion Technologies) and MECA 4 recording chips (recording chip with four 100-μm cavities, Nanion Technologies, #132002) were used for all electrophysiological measurements of purified hGluD2 , hGluD2 ΔATD , hGluD2 R710W , and hGluD2 R530K . .. The cleaved protein was further separated via size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (Cytiva, 29091596) equilibrated with the GDN buffer.

    Article Title: Delta-type glutamate receptors are ligand-gated ion channels.
    Article Snippet: This is a PDF file of a peer-reviewed paper that has been accepted for publication.. Although unedited, the content has been subjected to preliminary formatting.. Nature is providing this early version of the typeset paper as a service to our authors and readers.

    Article Title: Molecular basis for ligand-gating of the human GluD1 receptor
    Article Snippet: From this, model building, refinement, and structural analysis were carried out using a combination of ChimeraX , ISOLDE , Coot , and PHENIX , as compiled and distributed by the SBGrid Consortium , . .. The electrophysiological measurement of purified GluD1 was done on an Orbit mini plate form with a temperature control unit (Nanion Technologies), using MECA recording chips (4x 100 μm cavities, Nanion Technologies, 132002). ..

    Patch Clamp:

    Article Title: Evaluation of Possible Proarrhythmic Potency: Comparison of the Effect of Dofetilide, Cisapride, Sotalol, Terfenadine, and Verapamil on hERG and Native IKr Currents and on Cardiac Action Potential.
    Article Snippet: The proarrhythmic potency of drugs is usually attributed to the IKr current block.. During safety pharmacology testing analysis of IKr in cardiomyocytes was replaced by human ether-a-go-go-related gene (hERG) test using automated patch-clamp systems in stable transfected cell lines.. Aim of this study was to compare the effect of proarrhythmic compounds on hERG and IKr currents and on cardiac action potential.

    Article Title: Pharmacological activation of IKr in models of long QT Type 2 risks overcorrection of repolarization.
    Article Snippet: Cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific, Australia) and cultured as previously described7 (see Supplementary material online, Methods). .. Automated whole-cell patch-clamp recordings were performed using a Syncropatch 384 PE platform (Nanion Technologies, Munich, Germany) operated at room temperature (details provided in Supplementary material online, Methods). ..



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