cas9 polya plasmid (Addgene inc)

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Addgene inc cas9 polya plasmid
Cas9 Polya Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polya/pCas9-polyA+(Plasmid+%2372602)/pm41027500-46-2-4
Average 93 stars, based on 9 article reviews

cas9 polya plasmid - by Bioz Stars, 2026-11

93/100 stars

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Article Title: A high-quality severe combined immunodeficiency (SCID) rat bioresource.
Article Snippet: Electroporation of gRNA and Cas9 mRNA into rat zygotes Cas9 mRNA was transcribed in vitro using a mMESSAGE mMACHINE T7 Ultra Kit (Life Technologies, Grand Island, NY, USA) from linearized plasmid (pCas9-polyA, ID #72602; www.addgene.org/CRISPR); it was purified using a MEGAClear kit (Life Technologies).

Article Title: Opioidergic activation of the descending pain inhibitory system underlies placebo analgesia
Article Snippet: gRNA was prepared using a Precision gRNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA).

Article Title: Age-related ciliopathy: Obesogenic shortening of melanocortin-4 receptor-bearing neuronal primary cilia.
Article Snippet: Cas9 mRNA was transcribed in vitro using a mMESSAGE mMACHINE T7 Ultra Kit (Life Technologies) from a linearized plasmid (pCas9-polyA; Addgene, #72602) and was purified using a MEGAClear kit (Life Technologies).

Article Title: Humanized-Aquaporin-4-Expressing Rat Created by Gene-Editing Technology and Its Use to Clarify the Pathology of Neuromyelitis Optica Spectrum Disorder
Article Snippet: Cas9 mRNA was transcribed in vitro using a mMESSAGE mMACHINE T7 Ultra Kit (Thermo Fisher Scientific) from a linearized plasmid (Addgene plasmid # 72602; http://n2t.net/addgene:72602 (accessed on 23 July 2024); RRID:Addgene_72602) and was purified using a MEGAClear kit (Thermo Fisher Scientific).

Article Title: Opioidergic activation of the descending pain inhibitory system underlies placebo analgesia
Article Snippet: RNAscope Multiple Fluorescent Reagent Kit v2 Assay User manual for Fresh Frozen sections Advanced Cell Diagnostics (Newark, CA) #323100 RNAscope Probe-Mu opioid receptor 1 (oprm1), MOR Advanced Cell Diagnostics #410691 RNAscope Probe-Cre Advanced Cell Diagnostics #312281 TSA Cyanine 3 Perkin Elmer (Waltham, MA) NEL744001KT TSA Fluorescein Perkin Elmer NEL741001KT

Recombinant:

Article Title: Age-related ciliopathy: Obesogenic shortening of melanocortin-4 receptor-bearing neuronal primary cilia.
Article Snippet: Experimental models: Organisms/strains Rat: Slc:Wistar/ST Japan SLC N/A Rat: Iar:Wistar-Imamichi Institute for Animal Reproduction N/A Rat: Slc:Zucker Japan SLC lean Fa/fa and obese fa/fa Mc4r-deficient mice Balthasar et al.43 N/A Mouse: C57BL/6JJcl CLEA Japan N/A Guinea pigs Shimizu Experimental Materials N/A Oligonucleotides scrambled sequence (5’-GCGCTTAGCTGTAGGATTC TTCAAGAGAGAATCCTACAGCTAAGCGCTTTTTT-3’) Sigma-Aldrich Custom made Ift88 shRNA #1 (5’-GGCATTAGATACTTACAAATT CAAGAGATTTGTAAGTATCTAATGCCTTTTTT-3’) Sigma-Aldrich Custom made Ift88 shRNA #2 (5’-GTATTTCCCTTCTAACATTTTCA AGAGAAATGTTAGAAGGGAAATACTTTTTT-3’) Sigma-Aldrich Custom made Cilk1 shRNA (5’-CACAACCACGTGGCGGTGTAAT TCAAGAGATTACACCGCCACGTGGTTGTGTTTTTT-3’) Sigma-Aldrich Custom made Actb: forward 5’-CCACACTTTCTACAATGAGC-3’ and reverse 5’-ATACAGGGACAACACAGC-3’ Thermo Fisher Scientific Custom made (Continued on next page) Cell Metabolism 36, 1–15.e1–e10, May 7, 2024 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mc4r: forward 5’-GACGGAGGATGCTATGAG-3’ and reverse 5’-AGGTTCTTGTTCTTGGCTAT-3’ Thermo Fisher Scientific Custom made Recombinant DNA pCas9-polyA Addgene Cat # 72602 pAAV2-CMV-Flex-Flag-Adcy3M279I Vector Builder Custom made pcDNA3 Invitrogen N/A Software and algorithms CRISPOR http://crispor.tefor.net/ N/A siDirect http://sidirect2.rnai.jp N/A Imaris software version 9.6.0 Oxford Instruments RRID: SCR_007370 Spike 2, version 7.10 CED RRID: SCR_00903 Prism 7.04 GraphPad RRID: SCR_002798 Other Gap electrode Nepa Gene Cat # CUY520P5 Electroporator (NEPA21 Super Electroporator) Nepa Gene Cat # Nepa21 DNA Sequencer (Applied Biosystems 3130xl Genetic Analyzer) Thermo Fisher Scientific 3130xl-100 Epifluorescence microscope Nikon Eclipse 80i Confocal laser-scanning microscope Leica TCS SP8 Body composition analyser Hitachi EchoMRI-900 Metabolism-measuring system Muromachi Kikai MK-5000RQ 3D scanner Japan 3D printer Einscan SP Feedam Melquest cFDM-700AS Microsyringe Hamilton N/A Real-time PCR system Thermo Fisher Scientific StepOnePlus Battery-operated telemetric transmitter Data Science International F40-TT Telemetry system Data Science International PhysioTel and PONEMAH Needle-type thermocouple Physitemp MT-29/2 Amplifier for electrophysiological recordings Molecular Devices CyberAmp 380 Picospritzer III Parker Cat # 052-0500-900 ..

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Article Title: The role of cell-autonomous circadian oscillation of Cry transcription in circadian rhythm generation.
Article Snippet: .. The Cas9polyA plasmid (Addgene #72602) was used as the template for in vitro transcription using the mMESSAGE mMACHINE T7 kit (Thermo Fisher Scientific, MA, USA). .. The Cas9 mRNA was purified using the MEGAclear kit (Thermo Fisher Scientific). sgRNAs forCry1 E-box1 andCry2 E-box2were designed using an online program (https://www.benchling.com/) to predict unique target sites throughout the mouse genome.

Article Title: COXFA4L3 enhances mitochondrial complex IV function to boost ATP synthesis and drive sperm motility.
Article Snippet: .. Briefly, the Cas9-polyA plasmid (Addgene #72602, BRC#RDB13130) was used as a template for in vitro transcription using the mMESSAGE mMACHINE T7 kit (Thermo Fisher Scientific). .. The synthesized Cas9 mRNA was purified using a MEGAclear kit (Thermo Fisher Scientific).

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