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casrx  (Addgene inc)


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    Structured Review

    Addgene inc casrx
    Casrx, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polya/PB-CAG-CasRX-P2A-EGFP-BGH+PolyA+(Plasmid+%23154004)/pm36864102-279-4-8
    Average 91 stars, based on 1 article reviews
    casrx - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Expressing:

    Article Title: Transcription of MERVL retrotransposons is required for preimplantation embryo development.
    Article Snippet: .. The expression plasmids of CasRx (PB-CAG-CasRx-P2A-EGFP-BGH PolyA, #154004, Addgene) and gRNA (pXR003: CasRx gRNA cloning backbone, #109053, Addgene) were gifted from H. Yang and P. Hsu22,50. .. For optimal expression of CasRx in early stages of preimplantation embryos, the CAG promoter sequence in the CasRx-expressing plasmid was replaced with CBh promoter, which was obtained from pX330-U6-Chimeric_BB-CBh-hSpCas9 (pX330, gifted from F. Zhang, #42230, Addgene)51, using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs).

    Cloning:

    Article Title: Transcription of MERVL retrotransposons is required for preimplantation embryo development.
    Article Snippet: .. The expression plasmids of CasRx (PB-CAG-CasRx-P2A-EGFP-BGH PolyA, #154004, Addgene) and gRNA (pXR003: CasRx gRNA cloning backbone, #109053, Addgene) were gifted from H. Yang and P. Hsu22,50. .. For optimal expression of CasRx in early stages of preimplantation embryos, the CAG promoter sequence in the CasRx-expressing plasmid was replaced with CBh promoter, which was obtained from pX330-U6-Chimeric_BB-CBh-hSpCas9 (pX330, gifted from F. Zhang, #42230, Addgene)51, using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs).



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