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polya selection module  (New England Biolabs)


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    New England Biolabs polya selection module
    Polya Selection Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6816 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polya+selection/NEBNext+Poly(A)+mRNA+Magnetic+Isolation+Module/pm41824417-388-25-28
    Average 99 stars, based on 6816 article reviews
    polya selection module - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Molecular Phylogeny and Taxonomic Revision of the Dictyostelid Social Amoebae (Dictyostelia, Eumycetozoa).
    Article Snippet: Total RNA was extracted from 108 cells with the Qiagen RNeasy Midi Kit (#75144). .. Libraries were prepared using the NEBNext Ultra2 Directional RNA library kit (#E7760S) with the NEBNext Poly(A)mRNA Magnetic Isolation Module (#E7490S). ..

    Article Title: Integrated Single‐Myofibre Multi‐Omics Correlates Inflammatory State to Myosin Energetics in ICU‐Acquired Weakness
    Article Snippet: .. mRNA was captured from 10 μL of fibre lysate using the NEBNext Poly(A) mRNA Magnetic Isolation Module according to the manufacturer's instructions. ..

    Article Title: Disentangling microbiota-dependent and -independent impacts of antibiotics on host physiology.
    Article Snippet: The integrity of the isolated RNA was analysed using an Agilent High Sensitivity RNA ScreenTape Kit and TapeStation 4200 at the Genomic Technology Center of the Azrieli Faculty of Medicine at Bar-Ilan University. .. 1,000 ng total RNA was treated with a NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490L; New England Biolabs). .. RNA-seq libraries were produced using the NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770L; New England Biolabs).

    Article Title: The Maternal Blood Transcriptome Reflects Molecular Signatures Associated With Large Offspring Syndrome and Other Adverse Pregnancy Outcomes in Cattle
    Article Snippet: Sequencing was performed at the Interdisciplinary Center of Biotechnology Research NextGen Sequencing Core ( https://biotech.ufl.edu/next‐gen‐dna/ , RRID:SCR_019152). .. First, 500 ng of total RNA was used for mRNA isolation using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA, catalog #E7490). .. Then, RNA library construction was performed with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, catalog #E7760) with 2/3 of recommended poly A enriched RNA and 1/4 recommended reaction volume using SPT Labtech mosquito LV liquid handling instrument (Tecan, Männedorf, Switzerland).

    Article Title: Turning Red to Green Repeatedly: Ancestral LWS Duplications Drive Convergent Visual Tuning in Teleost Fishes
    Article Snippet: Total RNA concentrations were measured on a fluorometer (Qubit, ThermoFisher), and integrity was verified using a Pico chip on an Agilent 2100 Bioanalyzer ( https://www.agilent.com ). .. Library preparation (unstranded, 300 bp insert) was conducted using an Illumina NEBNext Ultra II Directional RNA library preparation kit, NEBNext Multiplex Oligos and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) for all osteoglossiform, tarpon and goby species used for transcriptomics except for O. lineolata , N. melanostomus and A. phalaena , whose library preparations were outsourced to Novogene, Singapore ( https://en.novogene.com/ ). .. Transcriptome sequencing (RNAseq, HiSeq, Paired‐End PE150) was outsourced to Novogene for all species.

    Article Title: The homologous GRAS transcription factors RAM1 and RAD1 coordinate shared and separate gene networks to regulate arbuscular mycorrhiza symbiosis.
    Article Snippet: RNA-sequencing For all experiments, RNA samples were quality-checked using gel electrophoresis and bioanalyzer (Agilent, Santa Clara, CA, USA) .. For the AM experiment, mRNAs were enriched using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswich, MA, USA). .. Libraries were prepared using NEBNextUltra RNALibrary Prep Kit for Illumina (NEB) and sequenced on aNovaSeq 6000 S4 platform by BGI, Hong Kong (paired-end 150 bp).

    Article Title: Ecology drives the degree of convergence in the gene expression of extremophile fishes
    Article Snippet: .. We then isolated mRNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Inc., Ipswich, MA, USA) and constructed cDNA libraries with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Inc., Ipswich, MA, USA). .. All cDNA libraries were individually barcoded, quantified using Qubit (Thermo Fisher Scientific, MA, USA) and an Agilent 2100 Bioanalyzer High Sensitivity DNA chip (Agilent, CA, USA), and pooled in sets of 11–12 samples based on nanomolar concentrations.

    Selection:

    Article Title: Brown Adipocyte BMAL1 Deficiency Is Associated with PVAT Remodeling and Increased Atherosclerosis.
    Article Snippet: .. RNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit (NEB #E7490, Ipswich, MA, USA) for Illumina with poly(A) selection and subjected to 151-bp paired-end sequencing on an Illumina NovaSeq platform (San Diego, CA, USA), generating an average of approximately 40 million paired reads per sample. ..

    Sequencing:

    Article Title: Brown Adipocyte BMAL1 Deficiency Is Associated with PVAT Remodeling and Increased Atherosclerosis.
    Article Snippet: .. RNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit (NEB #E7490, Ipswich, MA, USA) for Illumina with poly(A) selection and subjected to 151-bp paired-end sequencing on an Illumina NovaSeq platform (San Diego, CA, USA), generating an average of approximately 40 million paired reads per sample. ..

    RNA Library Preparation:

    Article Title: Turning Red to Green Repeatedly: Ancestral LWS Duplications Drive Convergent Visual Tuning in Teleost Fishes
    Article Snippet: Total RNA concentrations were measured on a fluorometer (Qubit, ThermoFisher), and integrity was verified using a Pico chip on an Agilent 2100 Bioanalyzer ( https://www.agilent.com ). .. Library preparation (unstranded, 300 bp insert) was conducted using an Illumina NEBNext Ultra II Directional RNA library preparation kit, NEBNext Multiplex Oligos and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) for all osteoglossiform, tarpon and goby species used for transcriptomics except for O. lineolata , N. melanostomus and A. phalaena , whose library preparations were outsourced to Novogene, Singapore ( https://en.novogene.com/ ). .. Transcriptome sequencing (RNAseq, HiSeq, Paired‐End PE150) was outsourced to Novogene for all species.

    Multiplex Assay:

    Article Title: Turning Red to Green Repeatedly: Ancestral LWS Duplications Drive Convergent Visual Tuning in Teleost Fishes
    Article Snippet: Total RNA concentrations were measured on a fluorometer (Qubit, ThermoFisher), and integrity was verified using a Pico chip on an Agilent 2100 Bioanalyzer ( https://www.agilent.com ). .. Library preparation (unstranded, 300 bp insert) was conducted using an Illumina NEBNext Ultra II Directional RNA library preparation kit, NEBNext Multiplex Oligos and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) for all osteoglossiform, tarpon and goby species used for transcriptomics except for O. lineolata , N. melanostomus and A. phalaena , whose library preparations were outsourced to Novogene, Singapore ( https://en.novogene.com/ ). .. Transcriptome sequencing (RNAseq, HiSeq, Paired‐End PE150) was outsourced to Novogene for all species.

    Transcriptomics:

    Article Title: Turning Red to Green Repeatedly: Ancestral LWS Duplications Drive Convergent Visual Tuning in Teleost Fishes
    Article Snippet: Total RNA concentrations were measured on a fluorometer (Qubit, ThermoFisher), and integrity was verified using a Pico chip on an Agilent 2100 Bioanalyzer ( https://www.agilent.com ). .. Library preparation (unstranded, 300 bp insert) was conducted using an Illumina NEBNext Ultra II Directional RNA library preparation kit, NEBNext Multiplex Oligos and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) for all osteoglossiform, tarpon and goby species used for transcriptomics except for O. lineolata , N. melanostomus and A. phalaena , whose library preparations were outsourced to Novogene, Singapore ( https://en.novogene.com/ ). .. Transcriptome sequencing (RNAseq, HiSeq, Paired‐End PE150) was outsourced to Novogene for all species.

    Construct:

    Article Title: Ecology drives the degree of convergence in the gene expression of extremophile fishes
    Article Snippet: .. We then isolated mRNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Inc., Ipswich, MA, USA) and constructed cDNA libraries with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Inc., Ipswich, MA, USA). .. All cDNA libraries were individually barcoded, quantified using Qubit (Thermo Fisher Scientific, MA, USA) and an Agilent 2100 Bioanalyzer High Sensitivity DNA chip (Agilent, CA, USA), and pooled in sets of 11–12 samples based on nanomolar concentrations.



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    (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 <t>mRNA.</t> Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.
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    Image Search Results


    (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 mRNA. Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.

    Journal: bioRxiv

    Article Title: Epstein-Barr Virus Latent Membrane Protein 1 Suppresses Ferroptosis via Pentose Phosphate Pathway and Glutathione Metabolism

    doi: 10.64898/2026.03.09.710628

    Figure Lengend Snippet: (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 mRNA. Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.

    Article Snippet: To construct indexed libraries, 1 μg of total RNA was used for polyA mRNA selection using NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat#E7490S), and library preparation with NEBNext Ultra RNA Library Prep with Sample Purification Beads (Cat#E7765S).

    Techniques: Infection, Activity Assay, RNA sequencing, Control, Expressing, Western Blot, Inhibition, Transduction, Selection