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strand-specific rna-seq with polya selection kit  (Illumina Inc)


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    Structured Review

    Illumina Inc strand-specific rna-seq with polya selection kit
    Strand Specific Rna Seq With Polya Selection Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polya+selection+kit/strand+specific+rna+seq+with+polya+selection+kit/pmc12208344-449-11-17
    Average 90 stars, based on 1 article reviews
    strand-specific rna-seq with polya selection kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    RNA Sequencing:

    Article Title: Senescence and inflammation are unintended adverse consequences of CRISPR-Cas9/AAV6-mediated gene editing in hematopoietic stem cells
    Article Snippet: .. 300 ng of total RNA was used for library preparation with strand-specific RNA-seq with PolyA selection kit (Illumina) for the RNA-seq in at day 1, TruSeq Stranded mRNA (Illumina) for the RNA-seq in at day 1 (paired-end), SMART-seq Ultra Low Input unstranded for RNA-seq in at day 4 (single-end). .. After primary antibodies incubation (Anti-53BP1 Antibody, Bethyl Laboratories; Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301 (γH2AX); NF-κB p65 (D14E12) XP Rabbit mAb, Cell signaling) cells were washed three times with DPBS and incubated with Alexa 568- and/or 647-labeled secondary antibodies (Invitrogen) (see ) diluted in 0.5% BSA and 0.2% fish gelatine in DPBS for 1 h in a dark humified chamber at RT.

    Article Title: Senescence and inflammation are unintended adverse consequences of CRISPR-Cas9/AAV6-mediated gene editing in hematopoietic stem cells.
    Article Snippet: .. 300 ng of total RNA was used for library preparation with strand-specific RNA-seq with PolyA selection kit (Illumina) for the RNA-seq in Figure 1 at day 1, TruSeq Stranded mRNA (Illumina) for the RNA-seq in Figure 4 at day 1 (paired-end), SMART-seq Ultra Low Input unstranded for RNA-seq in Figure 4 at day 4 (single-end). .. Samples were sequenced by the San Raffaele COSR or Genewiz (Azenta Life Sciences) using a NextSeq 500 High 75 (Illumina) or NoveSeq 2 x 150 bp sequencing configuration to obtain 10-30 x 10 6 reads per sample.

    Selection:

    Article Title: Senescence and inflammation are unintended adverse consequences of CRISPR-Cas9/AAV6-mediated gene editing in hematopoietic stem cells
    Article Snippet: .. 300 ng of total RNA was used for library preparation with strand-specific RNA-seq with PolyA selection kit (Illumina) for the RNA-seq in at day 1, TruSeq Stranded mRNA (Illumina) for the RNA-seq in at day 1 (paired-end), SMART-seq Ultra Low Input unstranded for RNA-seq in at day 4 (single-end). .. After primary antibodies incubation (Anti-53BP1 Antibody, Bethyl Laboratories; Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301 (γH2AX); NF-κB p65 (D14E12) XP Rabbit mAb, Cell signaling) cells were washed three times with DPBS and incubated with Alexa 568- and/or 647-labeled secondary antibodies (Invitrogen) (see ) diluted in 0.5% BSA and 0.2% fish gelatine in DPBS for 1 h in a dark humified chamber at RT.

    Article Title: Senescence and inflammation are unintended adverse consequences of CRISPR-Cas9/AAV6-mediated gene editing in hematopoietic stem cells.
    Article Snippet: .. 300 ng of total RNA was used for library preparation with strand-specific RNA-seq with PolyA selection kit (Illumina) for the RNA-seq in Figure 1 at day 1, TruSeq Stranded mRNA (Illumina) for the RNA-seq in Figure 4 at day 1 (paired-end), SMART-seq Ultra Low Input unstranded for RNA-seq in Figure 4 at day 4 (single-end). .. Samples were sequenced by the San Raffaele COSR or Genewiz (Azenta Life Sciences) using a NextSeq 500 High 75 (Illumina) or NoveSeq 2 x 150 bp sequencing configuration to obtain 10-30 x 10 6 reads per sample.



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