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Cellvis Inc poly d lysine coated
Poly D Lysine Coated, supplied by Cellvis Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+a/coated+d+lysine+poly/pm42313775-71-18-33
Average 86 stars, based on 1 article reviews
poly d lysine coated - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Recombinant:

Article Title: Protocol for cell cycle-resolved high-content imaging of transcription condensate dynamics in human cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-MED1 (1:500) Cell Signaling Technologies Cat# 51613S; RRID: AB_2799397 Mouse monoclonal anti-human NPAT (1:1000) BD Biosciences Cat# 611344; RRID: AB_398866 Alexa FluorTM 488-conjugated goat anti-rabbit IgG (H+L) (1:1000) Invitrogen Cat# A-11008 RRID: AB_143165 Alexa FluorTM 568-conjugated goat anti-mouse IgG (H+L) (1:1000) Invitrogen Cat# A-11004 RRID: AB_2534072 Chemicals, peptides, and recombinant proteins 5 ′ ethynyl 2 ′ deoxyuridine (EdU) Millipore Sigma Cat# 900584 DAPI Millipore Sigma Cat# D9542 Alexa FluorTM 647 Azide, Triethylammonium Salt Invitrogen Cat# A10277 GibcoTM DPBS, no calcium, no magnesium ThermoFisher Scientific Cat# 14190144 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences Cat# 15700 Bovine Serum Albumin (BSA) DNase- and Protease-free Powder Fisher Bioreagents BP9706100 Critical commercial assays Click-iTTM Cell Reaction buffer kit Invitrogen Cat# C10269 Experimental models: Cell lines Human: MCF10A cell line ATCC Cat# CRL-10317; RRID: CVCL_0598 Software and algorithms CellProfiler Stirling et al. 3 RRID: SCR_007358 ImageJ Schneider et al. 4 RRID: SCR_003070 CellProfiler pipeline Marmolejo et al. 1 Zenodo: https://doi.org/10.5281/ zenodo.18040849 Spotfire TIBCO RRID: SCR_008858 Other ImageXpress Micro Confocal High-Content Imaging System Molecular Devices RRID: SCR_020294 96 Well plate with #1.5 glass-like polymer coverslip bottom Cellvis Cat# P96-1.5P 2 STAR Protocols 7, 104589, June 19, 2026 ..

Electron Microscopy:

Article Title: Protocol for cell cycle-resolved high-content imaging of transcription condensate dynamics in human cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-MED1 (1:500) Cell Signaling Technologies Cat# 51613S; RRID: AB_2799397 Mouse monoclonal anti-human NPAT (1:1000) BD Biosciences Cat# 611344; RRID: AB_398866 Alexa FluorTM 488-conjugated goat anti-rabbit IgG (H+L) (1:1000) Invitrogen Cat# A-11008 RRID: AB_143165 Alexa FluorTM 568-conjugated goat anti-mouse IgG (H+L) (1:1000) Invitrogen Cat# A-11004 RRID: AB_2534072 Chemicals, peptides, and recombinant proteins 5 ′ ethynyl 2 ′ deoxyuridine (EdU) Millipore Sigma Cat# 900584 DAPI Millipore Sigma Cat# D9542 Alexa FluorTM 647 Azide, Triethylammonium Salt Invitrogen Cat# A10277 GibcoTM DPBS, no calcium, no magnesium ThermoFisher Scientific Cat# 14190144 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences Cat# 15700 Bovine Serum Albumin (BSA) DNase- and Protease-free Powder Fisher Bioreagents BP9706100 Critical commercial assays Click-iTTM Cell Reaction buffer kit Invitrogen Cat# C10269 Experimental models: Cell lines Human: MCF10A cell line ATCC Cat# CRL-10317; RRID: CVCL_0598 Software and algorithms CellProfiler Stirling et al. 3 RRID: SCR_007358 ImageJ Schneider et al. 4 RRID: SCR_003070 CellProfiler pipeline Marmolejo et al. 1 Zenodo: https://doi.org/10.5281/ zenodo.18040849 Spotfire TIBCO RRID: SCR_008858 Other ImageXpress Micro Confocal High-Content Imaging System Molecular Devices RRID: SCR_020294 96 Well plate with #1.5 glass-like polymer coverslip bottom Cellvis Cat# P96-1.5P 2 STAR Protocols 7, 104589, June 19, 2026 ..

Software:

Article Title: Protocol for cell cycle-resolved high-content imaging of transcription condensate dynamics in human cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-MED1 (1:500) Cell Signaling Technologies Cat# 51613S; RRID: AB_2799397 Mouse monoclonal anti-human NPAT (1:1000) BD Biosciences Cat# 611344; RRID: AB_398866 Alexa FluorTM 488-conjugated goat anti-rabbit IgG (H+L) (1:1000) Invitrogen Cat# A-11008 RRID: AB_143165 Alexa FluorTM 568-conjugated goat anti-mouse IgG (H+L) (1:1000) Invitrogen Cat# A-11004 RRID: AB_2534072 Chemicals, peptides, and recombinant proteins 5 ′ ethynyl 2 ′ deoxyuridine (EdU) Millipore Sigma Cat# 900584 DAPI Millipore Sigma Cat# D9542 Alexa FluorTM 647 Azide, Triethylammonium Salt Invitrogen Cat# A10277 GibcoTM DPBS, no calcium, no magnesium ThermoFisher Scientific Cat# 14190144 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences Cat# 15700 Bovine Serum Albumin (BSA) DNase- and Protease-free Powder Fisher Bioreagents BP9706100 Critical commercial assays Click-iTTM Cell Reaction buffer kit Invitrogen Cat# C10269 Experimental models: Cell lines Human: MCF10A cell line ATCC Cat# CRL-10317; RRID: CVCL_0598 Software and algorithms CellProfiler Stirling et al. 3 RRID: SCR_007358 ImageJ Schneider et al. 4 RRID: SCR_003070 CellProfiler pipeline Marmolejo et al. 1 Zenodo: https://doi.org/10.5281/ zenodo.18040849 Spotfire TIBCO RRID: SCR_008858 Other ImageXpress Micro Confocal High-Content Imaging System Molecular Devices RRID: SCR_020294 96 Well plate with #1.5 glass-like polymer coverslip bottom Cellvis Cat# P96-1.5P 2 STAR Protocols 7, 104589, June 19, 2026 ..

Imaging:

Article Title: Protocol for cell cycle-resolved high-content imaging of transcription condensate dynamics in human cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-MED1 (1:500) Cell Signaling Technologies Cat# 51613S; RRID: AB_2799397 Mouse monoclonal anti-human NPAT (1:1000) BD Biosciences Cat# 611344; RRID: AB_398866 Alexa FluorTM 488-conjugated goat anti-rabbit IgG (H+L) (1:1000) Invitrogen Cat# A-11008 RRID: AB_143165 Alexa FluorTM 568-conjugated goat anti-mouse IgG (H+L) (1:1000) Invitrogen Cat# A-11004 RRID: AB_2534072 Chemicals, peptides, and recombinant proteins 5 ′ ethynyl 2 ′ deoxyuridine (EdU) Millipore Sigma Cat# 900584 DAPI Millipore Sigma Cat# D9542 Alexa FluorTM 647 Azide, Triethylammonium Salt Invitrogen Cat# A10277 GibcoTM DPBS, no calcium, no magnesium ThermoFisher Scientific Cat# 14190144 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences Cat# 15700 Bovine Serum Albumin (BSA) DNase- and Protease-free Powder Fisher Bioreagents BP9706100 Critical commercial assays Click-iTTM Cell Reaction buffer kit Invitrogen Cat# C10269 Experimental models: Cell lines Human: MCF10A cell line ATCC Cat# CRL-10317; RRID: CVCL_0598 Software and algorithms CellProfiler Stirling et al. 3 RRID: SCR_007358 ImageJ Schneider et al. 4 RRID: SCR_003070 CellProfiler pipeline Marmolejo et al. 1 Zenodo: https://doi.org/10.5281/ zenodo.18040849 Spotfire TIBCO RRID: SCR_008858 Other ImageXpress Micro Confocal High-Content Imaging System Molecular Devices RRID: SCR_020294 96 Well plate with #1.5 glass-like polymer coverslip bottom Cellvis Cat# P96-1.5P 2 STAR Protocols 7, 104589, June 19, 2026 ..

Article Title: Transmembrane orientation and extracellular juxtamembrane residues of GP130 are critical for signal transduction.
Article Snippet: Mean fluorescence intensity (MFI) values for all samples were entered into PRISM 10 (GraphPad) to allow statistical comparison between cell lines. .. Live cell FRET imaging HEK293 cells were maintained in their normal growth medium and seeded into 0.1 mg/ml poly-D-lysine coated 35 mm glass bottom dishes with 20 mm micro-well number 1 cover glass (Cellvis) and incubated overnight. .. The next day, appropriate amount of each plasmid DNA was transfected into cells using 2 μl Attractene transfection reagent (QIAGEN) and cells were incubated for further 24 h. Following transfection, cells were provided with fresh medium without phenol red (Gibco) and imaged 6 h later.

Article Title: The inner membrane complex protein, IMC55, is dispensable for intraerythrocytic development of Plasmodium falciparum .
Article Snippet: .. After expansion, gels were imaged immediately using a poly-D-lysine-treated glass-bottom imaging dish (Cellvis) or were stored in 0.2% propyl gallate at 4°C for future imaging. .. Primary antibodies used were rat anti-HA (3F10; Roche; 1:50), rabbit anti-IMC1g (a gift from Jeff Dvorin; 1:250), and rabbit anti-GAP45 (a gift from Jeff Dvorin; 1:100) (71, 103).

Polymer:

Article Title: Protocol for cell cycle-resolved high-content imaging of transcription condensate dynamics in human cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-MED1 (1:500) Cell Signaling Technologies Cat# 51613S; RRID: AB_2799397 Mouse monoclonal anti-human NPAT (1:1000) BD Biosciences Cat# 611344; RRID: AB_398866 Alexa FluorTM 488-conjugated goat anti-rabbit IgG (H+L) (1:1000) Invitrogen Cat# A-11008 RRID: AB_143165 Alexa FluorTM 568-conjugated goat anti-mouse IgG (H+L) (1:1000) Invitrogen Cat# A-11004 RRID: AB_2534072 Chemicals, peptides, and recombinant proteins 5 ′ ethynyl 2 ′ deoxyuridine (EdU) Millipore Sigma Cat# 900584 DAPI Millipore Sigma Cat# D9542 Alexa FluorTM 647 Azide, Triethylammonium Salt Invitrogen Cat# A10277 GibcoTM DPBS, no calcium, no magnesium ThermoFisher Scientific Cat# 14190144 Paraformaldehyde 16% Aqueous Solution EM Grade Electron Microscopy Sciences Cat# 15700 Bovine Serum Albumin (BSA) DNase- and Protease-free Powder Fisher Bioreagents BP9706100 Critical commercial assays Click-iTTM Cell Reaction buffer kit Invitrogen Cat# C10269 Experimental models: Cell lines Human: MCF10A cell line ATCC Cat# CRL-10317; RRID: CVCL_0598 Software and algorithms CellProfiler Stirling et al. 3 RRID: SCR_007358 ImageJ Schneider et al. 4 RRID: SCR_003070 CellProfiler pipeline Marmolejo et al. 1 Zenodo: https://doi.org/10.5281/ zenodo.18040849 Spotfire TIBCO RRID: SCR_008858 Other ImageXpress Micro Confocal High-Content Imaging System Molecular Devices RRID: SCR_020294 96 Well plate with #1.5 glass-like polymer coverslip bottom Cellvis Cat# P96-1.5P 2 STAR Protocols 7, 104589, June 19, 2026 ..

Article Title: Protocol for cell cycle-resolved high-content imaging of transcription condensate dynamics in human cells
Article Snippet: ImageXpress Micro Confocal High-Content Imaging System , Molecular Devices , RRID: SCR_020294. .. 96 Well plate with #1.5 glass-like polymer coverslip bottom , Cellvis , Cat# P96-1.5P. ..

Incubation:

Article Title: Transmembrane orientation and extracellular juxtamembrane residues of GP130 are critical for signal transduction.
Article Snippet: Mean fluorescence intensity (MFI) values for all samples were entered into PRISM 10 (GraphPad) to allow statistical comparison between cell lines. .. Live cell FRET imaging HEK293 cells were maintained in their normal growth medium and seeded into 0.1 mg/ml poly-D-lysine coated 35 mm glass bottom dishes with 20 mm micro-well number 1 cover glass (Cellvis) and incubated overnight. .. The next day, appropriate amount of each plasmid DNA was transfected into cells using 2 μl Attractene transfection reagent (QIAGEN) and cells were incubated for further 24 h. Following transfection, cells were provided with fresh medium without phenol red (Gibco) and imaged 6 h later.

Article Title: Systematic profiling of cytosolic membraneless organelles reveals enrichment of oncogenic mRNA upon oxidative stress.
Article Snippet: .. Cells were seeded in a poly-lysine coated glass bottom M24-well plate (Cellvis, P24-1.5H-N) and left to settle overnight incubated at 37◦C, 5% CO2. .. The next day, media was changed to FluoroBrite DMEM (Gibco, A1896701) containing 1μg/mL Hoescht 33342 (Sigma-Aldrich, B2261) for 15 min to label the nuclei.

Glass:

Article Title: Protocol for cell cycle-resolved high-content imaging of transcription condensate dynamics in human cells
Article Snippet: ImageXpress Micro Confocal High-Content Imaging System , Molecular Devices , RRID: SCR_020294. .. 96 Well plate with #1.5 glass-like polymer coverslip bottom , Cellvis , Cat# P96-1.5P. ..



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