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A. Induced-fit docking pose <t>of</t> <t>GlcCer</t> with HRTV Gc protein. HRTV Gc ectodomain (PDB: 5YOW) is represented as ribbons and colored by domains (red, domain I; yellow, domain II; blue, domain III). The fusion loops at the tip of domain II are displayed in orange. The bound head group of GlcCer (framed) is drawn as sticks and colored according to atom type (carbon, green; nitrogen, blue; oxygen, red). B. Interaction of GlcCer with HRTV Gc protein in the lipid-head-group binding pocket. The hydrogen bonds between GlcCer and Gc protein are represented as dashed lines and colored in magenta. C. GlcCer binding groove. The HRTV Gc lipid-head-group binding pocket is shown in surface representation and colored according to electrostatic potential. D. 2D representation of docking interaction of GlcCer with binding site residues of HRTV Gc. The hydrogen bonds are shown in magenta lines. E. HRTV Gc protein directly binds to <t>DOPC,</t> GalCer, and GlcCer. Serially diluted DOPC, GalCer, and GlcCer were spotted onto the PVDF membrane. Protein-lipid overlay assay was performed with an anti-6xHis antibody. F. SPR assay of HRTV Gc binding kinetics to the indicated liposomes at neutral pH. All experiments were performed as duplicates, and the calculated fit is shown as a black line. Equilibrium constants (K D ) are determined by measuring the association rate constant (K a ) and dissociation rate constant (K d ). G. Binding kinetics of HRTV Gc to the indicated liposomes at acidic pH. Sensorgrams measured in replicates by bio-layer interferometry (BLI) and fitting (black) of the interaction of Gc to the indicated liposomes.

Dopc, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 99/100, based on 4981 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dopc - by Bioz Stars, 2026-08

99/100 stars

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1) Product Images from "Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion"

Article Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion

Journal: PLOS Pathogens

doi: 10.1371/journal.ppat.1011232

A. Induced-fit docking pose of GlcCer with HRTV Gc protein. HRTV Gc ectodomain (PDB: 5YOW) is represented as ribbons and colored by domains (red, domain I; yellow, domain II; blue, domain III). The fusion loops at the tip of domain II are displayed in orange. The bound head group of GlcCer (framed) is drawn as sticks and colored according to atom type (carbon, green; nitrogen, blue; oxygen, red). B. Interaction of GlcCer with HRTV Gc protein in the lipid-head-group binding pocket. The hydrogen bonds between GlcCer and Gc protein are represented as dashed lines and colored in magenta. C. GlcCer binding groove. The HRTV Gc lipid-head-group binding pocket is shown in surface representation and colored according to electrostatic potential. D. 2D representation of docking interaction of GlcCer with binding site residues of HRTV Gc. The hydrogen bonds are shown in magenta lines. E. HRTV Gc protein directly binds to DOPC, GalCer, and GlcCer. Serially diluted DOPC, GalCer, and GlcCer were spotted onto the PVDF membrane. Protein-lipid overlay assay was performed with an anti-6xHis antibody. F. SPR assay of HRTV Gc binding kinetics to the indicated liposomes at neutral pH. All experiments were performed as duplicates, and the calculated fit is shown as a black line. Equilibrium constants (K D ) are determined by measuring the association rate constant (K a ) and dissociation rate constant (K d ). G. Binding kinetics of HRTV Gc to the indicated liposomes at acidic pH. Sensorgrams measured in replicates by bio-layer interferometry (BLI) and fitting (black) of the interaction of Gc to the indicated liposomes.

Figure Legend Snippet: A. Induced-fit docking pose of GlcCer with HRTV Gc protein. HRTV Gc ectodomain (PDB: 5YOW) is represented as ribbons and colored by domains (red, domain I; yellow, domain II; blue, domain III). The fusion loops at the tip of domain II are displayed in orange. The bound head group of GlcCer (framed) is drawn as sticks and colored according to atom type (carbon, green; nitrogen, blue; oxygen, red). B. Interaction of GlcCer with HRTV Gc protein in the lipid-head-group binding pocket. The hydrogen bonds between GlcCer and Gc protein are represented as dashed lines and colored in magenta. C. GlcCer binding groove. The HRTV Gc lipid-head-group binding pocket is shown in surface representation and colored according to electrostatic potential. D. 2D representation of docking interaction of GlcCer with binding site residues of HRTV Gc. The hydrogen bonds are shown in magenta lines. E. HRTV Gc protein directly binds to DOPC, GalCer, and GlcCer. Serially diluted DOPC, GalCer, and GlcCer were spotted onto the PVDF membrane. Protein-lipid overlay assay was performed with an anti-6xHis antibody. F. SPR assay of HRTV Gc binding kinetics to the indicated liposomes at neutral pH. All experiments were performed as duplicates, and the calculated fit is shown as a black line. Equilibrium constants (K D ) are determined by measuring the association rate constant (K a ) and dissociation rate constant (K d ). G. Binding kinetics of HRTV Gc to the indicated liposomes at acidic pH. Sensorgrams measured in replicates by bio-layer interferometry (BLI) and fitting (black) of the interaction of Gc to the indicated liposomes.

Techniques Used: Binding Assay, Membrane, Protein-lipid Overlay Assay (PLOA), SPR Assay, Liposomes

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