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pnpp substrate solution (SouthernBiotech)

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SouthernBiotech pnpp substrate solution
Pnpp Substrate Solution, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pnpp+substrate+solution/pNPP+Substrate%2C+Tablet/pmc12643493-285-38-41
Average 93 stars, based on 80 article reviews

pnpp substrate solution - by Bioz Stars, 2026-11

93/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Intrinsic T cell glutaminolysis promotes autoimmunity in lupus-prone mice
Article Snippet: .. IgG was measured by ELISA using plates coated with goat anti-mouse IgG (Accurate Chemical) at a concentration of 1 μg/mL in 0.1 M bicarbonate buffer and detected with alkaline phosphatase-conjugated goat anti-mouse IgG (Millipore/Sigma) diluted 1:1,000 and of PNPP substrate solution (Southern Biotech). ..

Concentration Assay:

Incubation:

Article Title: A Shigella flexneri 2a synthetic glycan-based vaccine induces a long-lasting immune response in adults
Article Snippet: .. Wells were washed four times and 100 μl/well of phosphatase substrate, para -nitrophenyl phosphate (pNPP) one component (SouthernBiotech) was added and plates were incubated in the dark for 15 min at RT. ..

Clinical Proteomics:

Article Title: Loss of TET2 increases B-1 cell number and IgM production while limiting CDR3 diversity
Article Snippet: Briefly, EIA/RIA high-binding microplates were coated with goat anti-mouse IgM, capture antibody (Southern Biotech, 1020-01). .. Mouse IgM standards (Southern Biotech, 0101-01), or plasma samples were detected with alkaline phosphatase-conjugated goat anti-mouse IgM secondary antibody (Southern Biotech, 1020-04); and pNPP substrate (Southern Biotech 0201-01). .. Absorbance measurements were analyzed with a SpectraMAX 190 microplate reader (Molecular Devices) at 405 nm.