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msyk expression vector  (Addgene inc)


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    Structured Review

    Addgene inc msyk expression vector
    Msyk Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmscv-vector/pMSCV-mCherry-Syk+(Plasmid+%2350045)/pm41638907-237-1-7
    Average 92 stars, based on 9 article reviews
    msyk expression vector - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Fluorescence:


    Imaging:


    Microscopy:


    Plasmid Preparation:


    Article Title: Activating FcγR function depends on endosomal-signaling platforms
    Article Snippet: The chemiluminescence signal was acquired using a ChemiDoc Imaging System and the quantification was realized with the ImageLab software (BioRad). .. The pMSCV-mCherry-Syk plasmid (Addgene no. 50045) was used to express via a retrovirus mCherry-Syk in DC2.4 expressing the FcγRI-GFP. .. For retrovirus production 10 7 HEK-293-FT cells (Invitrogen), at 80% confluence, were transfected with a plasmid mix containing 10 μg of pMSCV-mCherry-Syk plasmid and 10 μg pCL-Ampho plasmid (Novusbio NBP2-29541) in 2.5 mL of transfection buffer (0.125M CaCl 2 , 25mM BES, 140mM NaCl, 0.75mM Na 2 HPO 4 ).

    Article Title: Syk activation during FcγR-mediated phagocytosis involves Syk palmitoylation and desulfenylation.
    Article Snippet: .. The mSyk expression vector was obtained from Addgene (#50045). .. FLAG-mSyk was prepared by inserting a FLAG epitope (DYKDDDDK) upstream of Syk coding sequence in the pCi vector (Promega).

    Article Title: Syk activation during FcγR-mediated phagocytosis involves Syk palmitoylation and desulfenylation
    Article Snippet: .. The mSyk expression vector was obtained from Addgene (#50045). .. FLAG-mSyk was prepared by inserting a FLAG epitope (DYKDDDDK) upstream of Syk coding sequence in the pCi vector (Promega).

    Article Title: Syk activation during FcγR-mediated phagocytosis involves both Syk palmitoylation and desulfenylation
    Article Snippet: .. mSyk expression vector was obtained from Addgene (#50045). .. FLAG-mSyk was prepared by inserting a FLAG epitope (DYKDDDDK) upstream Syk coding sequence in pCi vector (Promega).

    Article Title: TLR-mediated aggresome-like induced structures comprise antimicrobial peptides and attenuate intracellular bacterial survival
    Article Snippet: .. The percent phagocytosis and intracellular proliferation of bacteria (fold proliferation) was determined using the formulae: Percent phagocytosis = [CFU at 2 h]/[CFU of preinoculum]*100 Fold proliferation = [CFU at 16 hours]/[CFU at 2 hours] Plasmids, Antibodies and Reagents: The following plasmids used were obtained from Addgene, H2BpDendra2(N) (Addgene plasmid#: 75283), pMSCV-mCherry-Syk (Addgene plasmid#: 50045), pcDNA3.1 3xFlag IFIT1 (Addgene plasmid#: 53554), pCMV-HA-hIFITM2 (Addgene plasmid#: 58398), pFLAGTetherin (Addgene plasmid#: 41070), pRK5-FLAG-HBXIP (Addgene plasmid#: 42326). p-IRG1-FLAG was received as a generous gift from Dr. Karsten Hiller (Michelucci, A. et al., 2013). ..

    Article Title: TLR-mediated aggresome-like induced structures comprise antimicrobial peptides and attenuate intracellular bacterial survival
    Article Snippet: .. The percent phagocytosis and intracellular proliferation of bacteria (fold proliferation) was determined using the formulae: Percent phagocytosis = [CFU at 2 h]/[CFU of pre-inoculum]*100 Fold proliferation = [CFU at 16 hours]/[CFU at 2 hours] Plasmids, Antibodies and Reagents: The following plasmids used were obtained from Addgene, H2B-pDendra2(N) (Addgene plasmid#: 75283), pMSCV-mCherry-Syk (Addgene plasmid#: 50045), pcDNA3.1 3xFlag IFIT1 (Addgene plasmid#: 53554), pCMV-HAhIFITM2 (Addgene plasmid#: 58398), pFLAG-Tetherin (Addgene plasmid#: 41070), pRK5FLAG-HBXIP (Addgene plasmid#: 42326). ..

    Expressing:


    Article Title: Activating FcγR function depends on endosomal-signaling platforms
    Article Snippet: The chemiluminescence signal was acquired using a ChemiDoc Imaging System and the quantification was realized with the ImageLab software (BioRad). .. The pMSCV-mCherry-Syk plasmid (Addgene no. 50045) was used to express via a retrovirus mCherry-Syk in DC2.4 expressing the FcγRI-GFP. .. For retrovirus production 10 7 HEK-293-FT cells (Invitrogen), at 80% confluence, were transfected with a plasmid mix containing 10 μg of pMSCV-mCherry-Syk plasmid and 10 μg pCL-Ampho plasmid (Novusbio NBP2-29541) in 2.5 mL of transfection buffer (0.125M CaCl 2 , 25mM BES, 140mM NaCl, 0.75mM Na 2 HPO 4 ).

    Article Title: Syk activation during FcγR-mediated phagocytosis involves Syk palmitoylation and desulfenylation.
    Article Snippet: .. The mSyk expression vector was obtained from Addgene (#50045). .. FLAG-mSyk was prepared by inserting a FLAG epitope (DYKDDDDK) upstream of Syk coding sequence in the pCi vector (Promega).

    Article Title: Syk activation during FcγR-mediated phagocytosis involves Syk palmitoylation and desulfenylation
    Article Snippet: .. The mSyk expression vector was obtained from Addgene (#50045). .. FLAG-mSyk was prepared by inserting a FLAG epitope (DYKDDDDK) upstream of Syk coding sequence in the pCi vector (Promega).

    Article Title: Syk activation during FcγR-mediated phagocytosis involves both Syk palmitoylation and desulfenylation
    Article Snippet: .. mSyk expression vector was obtained from Addgene (#50045). .. FLAG-mSyk was prepared by inserting a FLAG epitope (DYKDDDDK) upstream Syk coding sequence in pCi vector (Promega).

    Bacteria:

    Article Title: TLR-mediated aggresome-like induced structures comprise antimicrobial peptides and attenuate intracellular bacterial survival
    Article Snippet: .. The percent phagocytosis and intracellular proliferation of bacteria (fold proliferation) was determined using the formulae: Percent phagocytosis = [CFU at 2 h]/[CFU of preinoculum]*100 Fold proliferation = [CFU at 16 hours]/[CFU at 2 hours] Plasmids, Antibodies and Reagents: The following plasmids used were obtained from Addgene, H2BpDendra2(N) (Addgene plasmid#: 75283), pMSCV-mCherry-Syk (Addgene plasmid#: 50045), pcDNA3.1 3xFlag IFIT1 (Addgene plasmid#: 53554), pCMV-HA-hIFITM2 (Addgene plasmid#: 58398), pFLAGTetherin (Addgene plasmid#: 41070), pRK5-FLAG-HBXIP (Addgene plasmid#: 42326). p-IRG1-FLAG was received as a generous gift from Dr. Karsten Hiller (Michelucci, A. et al., 2013). ..

    Article Title: TLR-mediated aggresome-like induced structures comprise antimicrobial peptides and attenuate intracellular bacterial survival
    Article Snippet: .. The percent phagocytosis and intracellular proliferation of bacteria (fold proliferation) was determined using the formulae: Percent phagocytosis = [CFU at 2 h]/[CFU of pre-inoculum]*100 Fold proliferation = [CFU at 16 hours]/[CFU at 2 hours] Plasmids, Antibodies and Reagents: The following plasmids used were obtained from Addgene, H2B-pDendra2(N) (Addgene plasmid#: 75283), pMSCV-mCherry-Syk (Addgene plasmid#: 50045), pcDNA3.1 3xFlag IFIT1 (Addgene plasmid#: 53554), pCMV-HAhIFITM2 (Addgene plasmid#: 58398), pFLAG-Tetherin (Addgene plasmid#: 41070), pRK5FLAG-HBXIP (Addgene plasmid#: 42326). ..



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    Image Search Results


    Design of human/murine hybrid epidermal growth factor receptor (EGFR) and EGFR murine chimeric antigen receptor (CAR). ( A ) Schematic of the human/murine hybrid EGFR with cetuximab-binding substitutions (G442S, R467K, M491I, N492S, K497N) and its engagement by a cetuximab-derived single-chain variable fragment (scFv) on mCAR T cells. ( B ) The retroviral vector encoding the EGFR-specific murine CAR and green fluorescent protein (GFP); I—domain I; II—domain II; III—domain III; IV—domain IV.

    Journal: International Journal of Molecular Sciences

    Article Title: Pre-Treatment with Dacarbazine Sensitizes B16 Melanoma to CAR T Cell Therapy in Syngeneic Mouse Model

    doi: 10.3390/ijms27010189

    Figure Lengend Snippet: Design of human/murine hybrid epidermal growth factor receptor (EGFR) and EGFR murine chimeric antigen receptor (CAR). ( A ) Schematic of the human/murine hybrid EGFR with cetuximab-binding substitutions (G442S, R467K, M491I, N492S, K497N) and its engagement by a cetuximab-derived single-chain variable fragment (scFv) on mCAR T cells. ( B ) The retroviral vector encoding the EGFR-specific murine CAR and green fluorescent protein (GFP); I—domain I; II—domain II; III—domain III; IV—domain IV.

    Article Snippet: The synthetic CAR gene was cloned by PCR amplification with primers containing restriction sites, restriction digest, and ligation (restriction enzymes and ligase from New England Biolabs, Ipswich, MA, USA) into the retroviral vector pMSCV (a gift from Dr. Reya, Addgene, #20672) right before IRES followed by the reporter gene of GFP.

    Techniques: Binding Assay, Derivative Assay, Retroviral, Plasmid Preparation

    Generation of murine EGFR CAR T cells. ( A ) Workflow for EGFR mCAR T cell production, including T cell isolation, activation, retroviral transduction, and subsequent in vitro and in vivo experiments created with BioRender ( https://www.biorender.com/ (accessed on 1 September 2025)). ( B ) Representative flow cytometry histogram showing the transduction efficiency of murine T cells with the EGFR-targeted murine CAR construct. ( C ) Summary of independent transduction preparations: percentage of GFP + cells in each preparation. ( D ) Expansion ( left ) and viability ( right ) of EGFR mCAR T cells compared to non-transduced T cells over 14 days of culture. * p < 0.05 and *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: Pre-Treatment with Dacarbazine Sensitizes B16 Melanoma to CAR T Cell Therapy in Syngeneic Mouse Model

    doi: 10.3390/ijms27010189

    Figure Lengend Snippet: Generation of murine EGFR CAR T cells. ( A ) Workflow for EGFR mCAR T cell production, including T cell isolation, activation, retroviral transduction, and subsequent in vitro and in vivo experiments created with BioRender ( https://www.biorender.com/ (accessed on 1 September 2025)). ( B ) Representative flow cytometry histogram showing the transduction efficiency of murine T cells with the EGFR-targeted murine CAR construct. ( C ) Summary of independent transduction preparations: percentage of GFP + cells in each preparation. ( D ) Expansion ( left ) and viability ( right ) of EGFR mCAR T cells compared to non-transduced T cells over 14 days of culture. * p < 0.05 and *** p < 0.001.

    Article Snippet: The synthetic CAR gene was cloned by PCR amplification with primers containing restriction sites, restriction digest, and ligation (restriction enzymes and ligase from New England Biolabs, Ipswich, MA, USA) into the retroviral vector pMSCV (a gift from Dr. Reya, Addgene, #20672) right before IRES followed by the reporter gene of GFP.

    Techniques: Cell Isolation, Activation Assay, Retroviral, Transduction, In Vitro, In Vivo, Flow Cytometry, Construct