retroviral plpcx vectors expressing the mitochondrial or cytoplasmic form of grx1–rogfp2 (Addgene inc)
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retroviral plpcx vectors expressing the mitochondrial or cytoplasmic form of grx1–rogfp2
Retroviral Plpcx Vectors Expressing The Mitochondrial Or Cytoplasmic Form Of Grx1–Rogfp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plpcx+expression+vector/plpcx+mito+grx1+rogfp2/pmc10245039-189-10-26
Average 90 stars, based on 1 article reviews
Retroviral Plpcx Vectors Expressing The Mitochondrial Or Cytoplasmic Form Of Grx1–Rogfp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plpcx+expression+vector/plpcx+mito+grx1+rogfp2/pmc10245039-189-10-26
Average 90 stars, based on 1 article reviews
retroviral plpcx vectors expressing the mitochondrial or cytoplasmic form of grx1–rogfp2 - by Bioz Stars,
2026-11
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Construct:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Plasmid Preparation:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Expressing:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Clone Assay:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Cloning:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Generated:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Imaging:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Cell Culture:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Stable Transfection:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Transfection:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Modification:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in Sequencing:Article Title: Mitoregulin Promotes Cell Cycle Progression in Non-Small Cell Lung Cancer Cells. Article Snippet: Cytosol- and mitochondria-localized roGFP2-Orp1 H2O2 sensors were subcloned from Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction Article Snippet: A similar imaging and analysis was used to measure H 2 O 2 in cultured neuroblastoma cell lines ectopically expressing the ratiometric sensors mito-roGFP2-orp1 and mito( Article Title: Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout. Article Snippet: 122 No. 19 e2503978122 https://doi.org/10.1073/pnas.2503978122 7 of 10 Molecular Biology. pRsetB vectors were used for bacterial expression; pAAV vectors under a CAG or a hSyn promoter were used for mammalian expression. pAAV- CAG- Grx1- RoTq- On was cloned from pAAV- CAG- LiLac, Article Title: Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction. Article Snippet: The elevated emission of reactive oxygen species (ROS) from presynaptic mitochondria is well-documented in several inflammatory and neurodegenerative diseases.. However, the potential role of mitochondrial ROS in presynaptic function and plasticity remains largely understudied beyond the context of disease.. Here, we investigated this potential ROS role in presynaptic function and short-term plasticity by combining optogenetics, whole cell electrophysiological recordings, and live confocal imaging using a well-established protocol for induction and measurement of synaptic potentiation in Drosophila melanogaster neuromuscular junctions (NMJ). Article Title: Chronic high-sugar diet in adulthood protects Caenorhabditis elegans from 6-OHDA-induced dopaminergic neurodegeneration Article Snippet: The mito-roGFP2-Grx1 coding sequence was adapted from Article Title: Multivalent binding of the tardigrade Dsup protein to chromatin promotes yeast survival and longevity upon exposure to oxidative damage Article Snippet: Plasmid p415TEF cyto roGFP2-Grx1-NLS was made from Article Title: Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging Article Snippet: After establishment of pALDH1A1-mNeptune stable cell line, cells were transfected with a pCMV-Grx1roGFP2-Hygromycin vector then the stable cell lines were selected with hygromycin B (the vector was modified from pEIGW-Grx1-roGFP2, 64990, Addgene). Article Title: Supporting Information for α-Lipoic acid ameliorates consequences of copper overload by upregulating selenoproteins and decreasing redox misbalance Article Snippet: To develop adenoviral constructs, NNLS-GRX1-roGFP2 cDNA was transferred to a multi-cloning site in |