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retroviral expression plasmid plpcx  (TaKaRa)


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    Structured Review

    TaKaRa retroviral expression plasmid plpcx
    Retroviral Expression Plasmid Plpcx, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plpcx+expression+vector/LRCX+Retroviral+Vector+Set/pmc07747745-161-7-11
    Average 95 stars, based on 26 article reviews
    retroviral expression plasmid plpcx - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: A molecular optomechanics approach reveals functional relevance of force transduction across talin and desmoplakin.
    Article Snippet: The following cDNA templates were used: human talin-1 (GeneBank BC042923), mCherry-AsLOV2 (Addgene #81041), mCherryAsLOV2(I427T) (Addgene #81035), Zdk2 (Addgene #81011), YPet (pCEP4-YPet; amino acids 1 to 228), PhoCl-mCherry (Addgene #87691), PhoCl2c-mCherry (Addgene #164037), and human DspII (Addgene #32227). .. Final cDNA sequences were assembled in expression vectors pLPCX or pLHCX (Clontech, 631511), N-terminal fragments of the dimers and full-length constructs in the former, and C-terminal fragments in the latter. ..

    Article Title: A conformational change in α-catenin's actin-binding domain governs adherens junction maturation.
    Article Snippet: .. Final cDNA sequences were assembled in the expression vector pLPCX (Clontech, 631511).Mutations in α-catenin (M319G, R326E) andα-catenin (L344P)42 were also created using the Gibson assembly approach. .. The correct cDNA sequences were confirmed by DNA sequencing (Eurofins, Microsynth Seqlab).

    Article Title: A molecular optomechanics approach reveals functional relevance of force transduction across talin and desmoplakin
    Article Snippet: The following cDNA templates were used: human talin-1 (GeneBank BC042923), mCherry-AsLOV2 (Addgene #81041), mCherry-AsLOV2(I427T) (Addgene #81035), Zdk2 (Addgene #81011), YPet (pCEP4-YPet; amino acids 1 to 228), PhoCl-mCherry (Addgene #87691), PhoCl2c-mCherry (Addgene #164037), and human DspII (Addgene #32227). .. Final cDNA sequences were assembled in expression vectors pLPCX or pLHCX (Clontech, 631511), N-terminal fragments of the dimers and full-length constructs in the former, and C-terminal fragments in the latter. ..

    Article Title: A conformational change in α-catenin’s actin-binding domain governs adherens junction maturation
    Article Snippet: .. Final cDNA sequences were assembled in the expression vector pLPCX (Clontech, 631511). ..

    Construct:

    Article Title: A molecular optomechanics approach reveals functional relevance of force transduction across talin and desmoplakin.
    Article Snippet: The following cDNA templates were used: human talin-1 (GeneBank BC042923), mCherry-AsLOV2 (Addgene #81041), mCherryAsLOV2(I427T) (Addgene #81035), Zdk2 (Addgene #81011), YPet (pCEP4-YPet; amino acids 1 to 228), PhoCl-mCherry (Addgene #87691), PhoCl2c-mCherry (Addgene #164037), and human DspII (Addgene #32227). .. Final cDNA sequences were assembled in expression vectors pLPCX or pLHCX (Clontech, 631511), N-terminal fragments of the dimers and full-length constructs in the former, and C-terminal fragments in the latter. ..

    Article Title: A molecular optomechanics approach reveals functional relevance of force transduction across talin and desmoplakin
    Article Snippet: The following cDNA templates were used: human talin-1 (GeneBank BC042923), mCherry-AsLOV2 (Addgene #81041), mCherry-AsLOV2(I427T) (Addgene #81035), Zdk2 (Addgene #81011), YPet (pCEP4-YPet; amino acids 1 to 228), PhoCl-mCherry (Addgene #87691), PhoCl2c-mCherry (Addgene #164037), and human DspII (Addgene #32227). .. Final cDNA sequences were assembled in expression vectors pLPCX or pLHCX (Clontech, 631511), N-terminal fragments of the dimers and full-length constructs in the former, and C-terminal fragments in the latter. ..

    other:

    Article Title: EGFR-phosphorylated GDH1 harmonizes with RSK2 to drive CREB activation and tumor metastasis in EGFR-activated lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: Hsd:Athymic Nude-Foxn1nu Envigo Cat#069 Mouse: 129S1/Svlmj The Jackson Laboratory Cat#002448 Mouse: C57BL/6JOlaHsd Envigo Cat#057 Oligonucleotides shRNA targeting sense sequence: RSK2 #1: CGCTGAGAATGGACAGCAAAT Horizon Discovery Cat#TRCN0000040144 shRNA targeting sense sequence: RSK2 #2: GCCTGAAGATACATTCTATTT Horizon Discovery Cat#TRCN0000040147 shRNA targeting sense sequence: GDH1 #1: GCCATTGAGAAAGTCTTCAAA Horizon Discovery Cat#TRCN0000028600 shRNA targeting sense sequence: GDH1 #2: CCCAAGAACTATACTGATAAT Horizon Discovery Cat#TRCN0000028588 shRNA targeting sense sequence: CREB #1: GCTCGATAAATCTAACAGTTA Horizon Discovery Cat#TRCN0000007308 shRNA targeting sense sequence: CREB #2: CGTCTAATGAAGAACAGGGAA Horizon Discovery Cat#TRCN0000007310 Primer: Fascin-1 Forward AGCCAGGGGCGGGACA Integrated DNA Technologies N/A Primer: Fascin-1 Reverse CGCTCCACAAAGCCCAGCTA Integrated DNA Technologies N/A Primer: PTK6 Forward TGCTCTGGAGCGCCTGT Integrated DNA Technologies N/A Primer: PTK6 Reverse TGTGGCCCAGCTGGAGCA Integrated DNA Technologies N/A Primer: ING3 Forward AGAAAACTACATTTCCCACAGAGG Integrated DNA Technologies N/A Primer: ING3 Reverse AAAAAAAACCACTTTGGCGCCTGAG Integrated DNA Technologies N/A Primer: GAPDH Forward GACATCAAGAAGGTGGTG Integrated DNA Technologies N/A Primer: GAPDH Reverse GTCATACCAGGAAATGAGC Integrated DNA Technologies N/A Recombinant DNA Plasmid: Gateway pDEST27 Invitrogen Cat#11812013 Plasmid: pLHCX Takara Cat#631511 Plasmid: pMSCV-hyg-Gateway This paper N/A Plasmid: pRSV-CaMKIV (1-313) Sun et al.35 Addgene plasmid: pRSV-CaMKIV(1-313); Cat#45063 Plasmid: pHAGE PGK-GFP-IRES-LUC-W Wilson et al.40 Addgene plasmid: pHAGE PGK-GFP-IRES- LUC-W; Cat#46793 Plasmid: pBabe-puro-EGFR WT Greulich et al.41 Addgene plasmid: EGFR WT; Cat#11011 Plasmid: pBabe-puro-EGFR D837A Greulich et al.41 Addgene plasmid: EGFR D837A; Cat#11014 Plasmid: pMSCV-myc-CREB S133D This paper N/A Plasmid: pMSCV-myc-CREB S133A This paper N/A Plasmid: pLHCX-flag-GDH1 WT This paper N/A Plasmid: pLHCX-flag-GDH1 Y135F This paper N/A Software and algorithms CompuSyn software ComboSyn Inc. https://www.combosyn.com/ ImageJ software National Institutes of Health https://imagej.nih.gov/ij/ GraphPad Prism software GraphPad Software http://www.graphpad.com Clinical annotation related to Figure 7 US Biomax http://www.biomax.us e4 Cell Reports 41, 111827, December 13, 2022

    Plasmid Preparation:

    Article Title: A conformational change in α-catenin's actin-binding domain governs adherens junction maturation.
    Article Snippet: .. Final cDNA sequences were assembled in the expression vector pLPCX (Clontech, 631511).Mutations in α-catenin (M319G, R326E) andα-catenin (L344P)42 were also created using the Gibson assembly approach. .. The correct cDNA sequences were confirmed by DNA sequencing (Eurofins, Microsynth Seqlab).

    Article Title: EGFR-phosphorylated GDH1 harmonizes with RSK2 to drive CREB activation and tumor metastasis in EGFR-activated lung cancer
    Article Snippet: Plasmid: Gateway pDEST27 , Invitrogen , Cat#11812013. .. Plasmid: pLHCX , Takara , Cat#631511. .. Plasmid: pMSCV-hyg-Gateway , This paper , N/A.

    Article Title: A conformational change in α-catenin’s actin-binding domain governs adherens junction maturation
    Article Snippet: .. Final cDNA sequences were assembled in the expression vector pLPCX (Clontech, 631511). ..

    Sequencing:

    Article Title: FOXC2 promotes vasculogenic mimicry and resistance to anti-angiogenic therapy.
    Article Snippet: The CellTag vector (pSMAL-CellTag-V1) was purchased from addgene (#115643), transduced cells were sorted on GFP. .. For cDNA over-expression, the coding sequence of murine Foxc2, fused to a C-terminal FLAG tag, was cloned into pLHCX (Clontech, 631511). .. For all experiments, infected cells were selected with 400 mg/mL Hygromycin (Thermo Fisher, 10687010) for 48 h then recovered in medium lacking antibiotic for at least two days.

    FLAG-tag:

    Article Title: FOXC2 promotes vasculogenic mimicry and resistance to anti-angiogenic therapy.
    Article Snippet: The CellTag vector (pSMAL-CellTag-V1) was purchased from addgene (#115643), transduced cells were sorted on GFP. .. For cDNA over-expression, the coding sequence of murine Foxc2, fused to a C-terminal FLAG tag, was cloned into pLHCX (Clontech, 631511). .. For all experiments, infected cells were selected with 400 mg/mL Hygromycin (Thermo Fisher, 10687010) for 48 h then recovered in medium lacking antibiotic for at least two days.

    Clone Assay:

    Article Title: FOXC2 promotes vasculogenic mimicry and resistance to anti-angiogenic therapy.
    Article Snippet: The CellTag vector (pSMAL-CellTag-V1) was purchased from addgene (#115643), transduced cells were sorted on GFP. .. For cDNA over-expression, the coding sequence of murine Foxc2, fused to a C-terminal FLAG tag, was cloned into pLHCX (Clontech, 631511). .. For all experiments, infected cells were selected with 400 mg/mL Hygromycin (Thermo Fisher, 10687010) for 48 h then recovered in medium lacking antibiotic for at least two days.



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