Review



plpcx empty vector  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    TaKaRa plpcx empty vector
    KEY RESOURCES TABLE
    Plpcx Empty Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plpcx+empty+vector/LRCX+Retroviral+Vector+Set/pmc08666140-78-0-4
    Average 95 stars, based on 26 article reviews
    plpcx empty vector - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "TRIM5α Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation"

    Article Title: TRIM5α Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation

    Journal: Cell reports

    doi: 10.1016/j.celrep.2019.05.040

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Transduction, Recombinant, Modification, Protease Inhibitor, Staining, Transfection, Clone Assay, Construct, shRNA, Sequencing, Luciferase, Plasmid Preparation, Expressing, Software, Imaging, Western Blot

    Related Articles

    Expressing:

    Article Title: A molecular optomechanics approach reveals functional relevance of force transduction across talin and desmoplakin.
    Article Snippet: The following cDNA templates were used: human talin-1 (GeneBank BC042923), mCherry-AsLOV2 (Addgene #81041), mCherryAsLOV2(I427T) (Addgene #81035), Zdk2 (Addgene #81011), YPet (pCEP4-YPet; amino acids 1 to 228), PhoCl-mCherry (Addgene #87691), PhoCl2c-mCherry (Addgene #164037), and human DspII (Addgene #32227). .. Final cDNA sequences were assembled in expression vectors pLPCX or pLHCX (Clontech, 631511), N-terminal fragments of the dimers and full-length constructs in the former, and C-terminal fragments in the latter. ..

    Article Title: A conformational change in α-catenin's actin-binding domain governs adherens junction maturation.
    Article Snippet: .. Final cDNA sequences were assembled in the expression vector pLPCX (Clontech, 631511).Mutations in α-catenin (M319G, R326E) andα-catenin (L344P)42 were also created using the Gibson assembly approach. .. The correct cDNA sequences were confirmed by DNA sequencing (Eurofins, Microsynth Seqlab).

    Article Title: A molecular optomechanics approach reveals functional relevance of force transduction across talin and desmoplakin
    Article Snippet: The following cDNA templates were used: human talin-1 (GeneBank BC042923), mCherry-AsLOV2 (Addgene #81041), mCherry-AsLOV2(I427T) (Addgene #81035), Zdk2 (Addgene #81011), YPet (pCEP4-YPet; amino acids 1 to 228), PhoCl-mCherry (Addgene #87691), PhoCl2c-mCherry (Addgene #164037), and human DspII (Addgene #32227). .. Final cDNA sequences were assembled in expression vectors pLPCX or pLHCX (Clontech, 631511), N-terminal fragments of the dimers and full-length constructs in the former, and C-terminal fragments in the latter. ..

    Article Title: A conformational change in α-catenin’s actin-binding domain governs adherens junction maturation
    Article Snippet: .. Final cDNA sequences were assembled in the expression vector pLPCX (Clontech, 631511). ..

    Construct:

    Article Title: A molecular optomechanics approach reveals functional relevance of force transduction across talin and desmoplakin.
    Article Snippet: The following cDNA templates were used: human talin-1 (GeneBank BC042923), mCherry-AsLOV2 (Addgene #81041), mCherryAsLOV2(I427T) (Addgene #81035), Zdk2 (Addgene #81011), YPet (pCEP4-YPet; amino acids 1 to 228), PhoCl-mCherry (Addgene #87691), PhoCl2c-mCherry (Addgene #164037), and human DspII (Addgene #32227). .. Final cDNA sequences were assembled in expression vectors pLPCX or pLHCX (Clontech, 631511), N-terminal fragments of the dimers and full-length constructs in the former, and C-terminal fragments in the latter. ..

    Article Title: A molecular optomechanics approach reveals functional relevance of force transduction across talin and desmoplakin
    Article Snippet: The following cDNA templates were used: human talin-1 (GeneBank BC042923), mCherry-AsLOV2 (Addgene #81041), mCherry-AsLOV2(I427T) (Addgene #81035), Zdk2 (Addgene #81011), YPet (pCEP4-YPet; amino acids 1 to 228), PhoCl-mCherry (Addgene #87691), PhoCl2c-mCherry (Addgene #164037), and human DspII (Addgene #32227). .. Final cDNA sequences were assembled in expression vectors pLPCX or pLHCX (Clontech, 631511), N-terminal fragments of the dimers and full-length constructs in the former, and C-terminal fragments in the latter. ..

    other:

    Article Title: EGFR-phosphorylated GDH1 harmonizes with RSK2 to drive CREB activation and tumor metastasis in EGFR-activated lung cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: Hsd:Athymic Nude-Foxn1nu Envigo Cat#069 Mouse: 129S1/Svlmj The Jackson Laboratory Cat#002448 Mouse: C57BL/6JOlaHsd Envigo Cat#057 Oligonucleotides shRNA targeting sense sequence: RSK2 #1: CGCTGAGAATGGACAGCAAAT Horizon Discovery Cat#TRCN0000040144 shRNA targeting sense sequence: RSK2 #2: GCCTGAAGATACATTCTATTT Horizon Discovery Cat#TRCN0000040147 shRNA targeting sense sequence: GDH1 #1: GCCATTGAGAAAGTCTTCAAA Horizon Discovery Cat#TRCN0000028600 shRNA targeting sense sequence: GDH1 #2: CCCAAGAACTATACTGATAAT Horizon Discovery Cat#TRCN0000028588 shRNA targeting sense sequence: CREB #1: GCTCGATAAATCTAACAGTTA Horizon Discovery Cat#TRCN0000007308 shRNA targeting sense sequence: CREB #2: CGTCTAATGAAGAACAGGGAA Horizon Discovery Cat#TRCN0000007310 Primer: Fascin-1 Forward AGCCAGGGGCGGGACA Integrated DNA Technologies N/A Primer: Fascin-1 Reverse CGCTCCACAAAGCCCAGCTA Integrated DNA Technologies N/A Primer: PTK6 Forward TGCTCTGGAGCGCCTGT Integrated DNA Technologies N/A Primer: PTK6 Reverse TGTGGCCCAGCTGGAGCA Integrated DNA Technologies N/A Primer: ING3 Forward AGAAAACTACATTTCCCACAGAGG Integrated DNA Technologies N/A Primer: ING3 Reverse AAAAAAAACCACTTTGGCGCCTGAG Integrated DNA Technologies N/A Primer: GAPDH Forward GACATCAAGAAGGTGGTG Integrated DNA Technologies N/A Primer: GAPDH Reverse GTCATACCAGGAAATGAGC Integrated DNA Technologies N/A Recombinant DNA Plasmid: Gateway pDEST27 Invitrogen Cat#11812013 Plasmid: pLHCX Takara Cat#631511 Plasmid: pMSCV-hyg-Gateway This paper N/A Plasmid: pRSV-CaMKIV (1-313) Sun et al.35 Addgene plasmid: pRSV-CaMKIV(1-313); Cat#45063 Plasmid: pHAGE PGK-GFP-IRES-LUC-W Wilson et al.40 Addgene plasmid: pHAGE PGK-GFP-IRES- LUC-W; Cat#46793 Plasmid: pBabe-puro-EGFR WT Greulich et al.41 Addgene plasmid: EGFR WT; Cat#11011 Plasmid: pBabe-puro-EGFR D837A Greulich et al.41 Addgene plasmid: EGFR D837A; Cat#11014 Plasmid: pMSCV-myc-CREB S133D This paper N/A Plasmid: pMSCV-myc-CREB S133A This paper N/A Plasmid: pLHCX-flag-GDH1 WT This paper N/A Plasmid: pLHCX-flag-GDH1 Y135F This paper N/A Software and algorithms CompuSyn software ComboSyn Inc. https://www.combosyn.com/ ImageJ software National Institutes of Health https://imagej.nih.gov/ij/ GraphPad Prism software GraphPad Software http://www.graphpad.com Clinical annotation related to Figure 7 US Biomax http://www.biomax.us e4 Cell Reports 41, 111827, December 13, 2022

    Plasmid Preparation:

    Article Title: A conformational change in α-catenin's actin-binding domain governs adherens junction maturation.
    Article Snippet: .. Final cDNA sequences were assembled in the expression vector pLPCX (Clontech, 631511).Mutations in α-catenin (M319G, R326E) andα-catenin (L344P)42 were also created using the Gibson assembly approach. .. The correct cDNA sequences were confirmed by DNA sequencing (Eurofins, Microsynth Seqlab).

    Article Title: EGFR-phosphorylated GDH1 harmonizes with RSK2 to drive CREB activation and tumor metastasis in EGFR-activated lung cancer
    Article Snippet: Plasmid: Gateway pDEST27 , Invitrogen , Cat#11812013. .. Plasmid: pLHCX , Takara , Cat#631511. .. Plasmid: pMSCV-hyg-Gateway , This paper , N/A.

    Article Title: A conformational change in α-catenin’s actin-binding domain governs adherens junction maturation
    Article Snippet: .. Final cDNA sequences were assembled in the expression vector pLPCX (Clontech, 631511). ..

    Sequencing:

    Article Title: FOXC2 promotes vasculogenic mimicry and resistance to anti-angiogenic therapy.
    Article Snippet: The CellTag vector (pSMAL-CellTag-V1) was purchased from addgene (#115643), transduced cells were sorted on GFP. .. For cDNA over-expression, the coding sequence of murine Foxc2, fused to a C-terminal FLAG tag, was cloned into pLHCX (Clontech, 631511). .. For all experiments, infected cells were selected with 400 mg/mL Hygromycin (Thermo Fisher, 10687010) for 48 h then recovered in medium lacking antibiotic for at least two days.

    FLAG-tag:

    Article Title: FOXC2 promotes vasculogenic mimicry and resistance to anti-angiogenic therapy.
    Article Snippet: The CellTag vector (pSMAL-CellTag-V1) was purchased from addgene (#115643), transduced cells were sorted on GFP. .. For cDNA over-expression, the coding sequence of murine Foxc2, fused to a C-terminal FLAG tag, was cloned into pLHCX (Clontech, 631511). .. For all experiments, infected cells were selected with 400 mg/mL Hygromycin (Thermo Fisher, 10687010) for 48 h then recovered in medium lacking antibiotic for at least two days.

    Clone Assay:

    Article Title: FOXC2 promotes vasculogenic mimicry and resistance to anti-angiogenic therapy.
    Article Snippet: The CellTag vector (pSMAL-CellTag-V1) was purchased from addgene (#115643), transduced cells were sorted on GFP. .. For cDNA over-expression, the coding sequence of murine Foxc2, fused to a C-terminal FLAG tag, was cloned into pLHCX (Clontech, 631511). .. For all experiments, infected cells were selected with 400 mg/mL Hygromycin (Thermo Fisher, 10687010) for 48 h then recovered in medium lacking antibiotic for at least two days.



    Similar Products

    95
    TaKaRa plpcx empty vector
    KEY RESOURCES TABLE
    Plpcx Empty Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plpcx+empty+vector/LRCX+Retroviral+Vector+Set/pmc08666140-78-0-4
    Average 95 stars, based on 1 article reviews
    plpcx empty vector - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    TaKaRa paper tgccaagcatgcctcactgcaa recombinant dna plpcx empty vector clontech
    KEY RESOURCES TABLE
    Paper Tgccaagcatgcctcactgcaa Recombinant Dna Plpcx Empty Vector Clontech, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plpcx+empty+vector/LRCX+Retroviral+Vector+Set/pm31189110-196-54-61
    Average 95 stars, based on 1 article reviews
    paper tgccaagcatgcctcactgcaa recombinant dna plpcx empty vector clontech - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Addgene inc plpcx empty vector
    KEY RESOURCES TABLE
    Plpcx Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plpcx+empty+vector/pcDNA3%2E1-(empty)-TAG+(Plasmid+%23138209)/pmc07265331-259-10-13
    Average 95 stars, based on 1 article reviews
    plpcx empty vector - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: TRIM5α Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation

    doi: 10.1016/j.celrep.2019.05.040

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: pLPCX Empty Vector , Clontech , Cat #519 631511.

    Techniques: Transduction, Recombinant, Modification, Protease Inhibitor, Staining, Transfection, Clone Assay, Construct, shRNA, Sequencing, Luciferase, Plasmid Preparation, Expressing, Software, Imaging, Western Blot