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Sangon Biotech sanprep column plasmid mini preps kit
Sanprep Column Plasmid Mini Preps Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/column+dna+extraction+gel+kit+sanprep/pmc12907642-86-9-25
Average 86 stars, based on 1 article reviews
sanprep column plasmid mini preps kit - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Clinical Proteomics:

Article Title: Fragmentation Analysis of the MYC Enhancer in Cell-Free DNA by qPCR for Early Detection of Hepatocellular Carcinoma.
Article Snippet: Background: The early detection of hepatocellular carcinoma (HCC) remains a significant challenge.. This study aimed to develop a quantitative PCR (qPCR) assay to analyze the fragment size distribution of cfDNA at the enhancer region of the oncogene MYC, evaluate its potential ability as a diagnostic biomarker for HCC.. Methods: In this retrospective case‐control study, we enrolled 40 patients with newly diagnosed HCC, 45 patients with cirrhosis, and 130 healthy volunteers.

Extraction:

Article Title: Fragmentation Analysis of the MYC Enhancer in Cell-Free DNA by qPCR for Early Detection of Hepatocellular Carcinoma.
Article Snippet: Background: The early detection of hepatocellular carcinoma (HCC) remains a significant challenge.. This study aimed to develop a quantitative PCR (qPCR) assay to analyze the fragment size distribution of cfDNA at the enhancer region of the oncogene MYC, evaluate its potential ability as a diagnostic biomarker for HCC.. Methods: In this retrospective case‐control study, we enrolled 40 patients with newly diagnosed HCC, 45 patients with cirrhosis, and 130 healthy volunteers.

Article Title: Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system
Article Snippet: Point mutations were introduced using the Mut Express II Fast Mutagenesis kit V2 (C214; Vazyme). .. Plasmid extraction and gel recovery were performed using the SanPrep Column Plasmid Mini-Preps kit (B518191) and the SanPrep Column DNA Gel extraction kit (B518131) from Sangon Biotech, respectively. .. Final constructed vectors were verified via Sanger sequencing by Sangon Biotech.

DNA Extraction:

Article Title: De novo design of dual-target mini-binders simultaneously neutralizing Streptococcus equi subspecies zooepidemicus M-like protein and host TNFR1 confers complete protection against lethal infection
Article Snippet: .. Briefly, the genome of SEZ was extracted by using Rapid Bacterial Genomic DNA Isolation Kit (B518225, Sangon Biotech, China), and the gene of SzM protein was amplified using specific primers. .. Then the gene was cloned into pET-22b vectors (69744-3, Addgene) by In-Fusion cloning, and the protein was expressed and purified as described above.

Article Title: In situ continuous evolution of native transporter HGT1 unlocks xylose utilization in Kluyveromyces lactis
Article Snippet: .. Genomic DNA was extracted using the SanPrep Column Yeast Genomic DNA Extraction Kit (Sangon Biotech). ..

Amplification:

Article Title: De novo design of dual-target mini-binders simultaneously neutralizing Streptococcus equi subspecies zooepidemicus M-like protein and host TNFR1 confers complete protection against lethal infection
Article Snippet: .. Briefly, the genome of SEZ was extracted by using Rapid Bacterial Genomic DNA Isolation Kit (B518225, Sangon Biotech, China), and the gene of SzM protein was amplified using specific primers. .. Then the gene was cloned into pET-22b vectors (69744-3, Addgene) by In-Fusion cloning, and the protein was expressed and purified as described above.

Purification:

Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus
Article Snippet: .. Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai), and first-strand cDNA was synthesized using ToloScript All-in-one RT EasyMix for qPCR (Tolo Biotech, Shanghai) following the manufacturers’ instructions. .. RT-qPCR was performed with 2 × Q3 SYBR qPCR Master Mix (Tolo Biotech, Shanghai) on a LightCycler 96 Real-Time PCR System (Roche, Switzerland) using gene-specific primers ( ).

Article Title: Metabolic and transport engineering enable efficient de novo biosynthesis of patchoulol in Saccharomyces cerevisiae
Article Snippet: .. Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai, China) according to the manufacturer's instructions. ..

Article Title: In situ continuous evolution of native transporter HGT1 unlocks xylose utilization in Kluyveromyces lactis
Article Snippet: .. The PCR products were purified using the SanPrep Column DNA Gel Extraction Kit (Sangon Biotech). .. Library construction, quality control, and high-throughput sequencing were performed on the Illumina NovaSeq platform by GENEWIZ (Suzhou, China).

Synthesized:

Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus
Article Snippet: .. Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai), and first-strand cDNA was synthesized using ToloScript All-in-one RT EasyMix for qPCR (Tolo Biotech, Shanghai) following the manufacturers’ instructions. .. RT-qPCR was performed with 2 × Q3 SYBR qPCR Master Mix (Tolo Biotech, Shanghai) on a LightCycler 96 Real-Time PCR System (Roche, Switzerland) using gene-specific primers ( ).

Real-time Polymerase Chain Reaction:

Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus
Article Snippet: .. Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai), and first-strand cDNA was synthesized using ToloScript All-in-one RT EasyMix for qPCR (Tolo Biotech, Shanghai) following the manufacturers’ instructions. .. RT-qPCR was performed with 2 × Q3 SYBR qPCR Master Mix (Tolo Biotech, Shanghai) on a LightCycler 96 Real-Time PCR System (Roche, Switzerland) using gene-specific primers ( ).

Polymerase Chain Reaction:

Article Title: In situ continuous evolution of native transporter HGT1 unlocks xylose utilization in Kluyveromyces lactis
Article Snippet: .. The PCR products were purified using the SanPrep Column DNA Gel Extraction Kit (Sangon Biotech). .. Library construction, quality control, and high-throughput sequencing were performed on the Illumina NovaSeq platform by GENEWIZ (Suzhou, China).

Gel Extraction:

Article Title: In situ continuous evolution of native transporter HGT1 unlocks xylose utilization in Kluyveromyces lactis
Article Snippet: .. The PCR products were purified using the SanPrep Column DNA Gel Extraction Kit (Sangon Biotech). .. Library construction, quality control, and high-throughput sequencing were performed on the Illumina NovaSeq platform by GENEWIZ (Suzhou, China).

Article Title: Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system
Article Snippet: Point mutations were introduced using the Mut Express II Fast Mutagenesis kit V2 (C214; Vazyme). .. Plasmid extraction and gel recovery were performed using the SanPrep Column Plasmid Mini-Preps kit (B518191) and the SanPrep Column DNA Gel extraction kit (B518131) from Sangon Biotech, respectively. .. Final constructed vectors were verified via Sanger sequencing by Sangon Biotech.

Plasmid Preparation:

Article Title: Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system
Article Snippet: Point mutations were introduced using the Mut Express II Fast Mutagenesis kit V2 (C214; Vazyme). .. Plasmid extraction and gel recovery were performed using the SanPrep Column Plasmid Mini-Preps kit (B518191) and the SanPrep Column DNA Gel extraction kit (B518131) from Sangon Biotech, respectively. .. Final constructed vectors were verified via Sanger sequencing by Sangon Biotech.



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