plasmids encoding teluc (Addgene inc)
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plasmids encoding teluc
Plasmids Encoding Teluc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+encoding+teluc/pcDNA3-teLuc+c-myc+(Plasmid+%23100026)/pm41916304-308-0-3
Average 93 stars, based on 5 article reviews
Plasmids Encoding Teluc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+encoding+teluc/pcDNA3-teLuc+c-myc+(Plasmid+%23100026)/pm41916304-308-0-3
Average 93 stars, based on 5 article reviews
plasmids encoding teluc - by Bioz Stars,
2026-11
93/100 stars
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Plasmid Preparation:Article Title: Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging Article Snippet: .. The Article Title: The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Sinorhizobium meliloti 1021 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Agrobacterium rhizogenes MSU440 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Biological samples Medicago truncatula Jemalog A17 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Medicago truncatula sunn-4 mutant Dr. Julia Frugoli, Clemson University N/A Chemicals, peptides, and recombinant proteins Protector RNase Inhibitor Sigma Aldrich SKU #3335399001 DAPI (40,6-diamidino-2-phenylindole) Sigma Aldrich PubChem Substance ID 24894305, SKU #D9542-5MG BD FACS Flow Sheath Buffer BD Biosciences catalog number: 342003 RNase inhibitor RibolockTM Thermo Fisher Scientific catalog number: EO0382 Critical commercial assays CYG germination pouches https://mega-international.com/tech-info/ N/A Polypap stands https://mega-international.com/tech-info/ N/A Humidity dome Alberta LTD Amazon Standard Identification Number: B089Q24D3N 40 mm cell strainer Greiner Bio-One Item No.: 542040 Miracloth Calbiochem Cat# 475855 Chromium Next GEM Chip G Single Cell Kit, 16 rxn 103 Genomics 1000127 MoClo Toolkit Addgene Kit #1000000044 Kulzer Technovit 7100 resin Electron Microscopy Sceinces 14653 Phusion Taq NEB M0530S Monarch DNA Plasmid Prep Kit NEB T1010S pENTR Directional TOPO vector Thermo Fisher K240020 Gatewa LR Clonas II Enzyme mix Thermo Fisher 11791100 10X Genomics Library Construction Kit 10X Genomics 1000190 10X Genomics Chromium Next GEM Single Cell 30 Kit v3.1, 4 Article Title: AAV-mediated BDNF and GAS6 muscle delivery delays disease onset in SOD1 G93A ALS mice. Article Snippet: .. MATERIALS AND METHODS Vector plasmid construction pAAV plasmidswere generated using the pAAV-CMV-teLuc backbonewhichwas constructed by substituting the eGFP gene from the pAAV-CMV-eGFP (Addgene Cat. 67634) with a teLuc gene PCR fragment, utilizing Article Title: Expanded applications of bioluminescence microscopy with phasor analysis. Article Snippet: .. Virus:Article Title: The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Sinorhizobium meliloti 1021 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Agrobacterium rhizogenes MSU440 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Biological samples Medicago truncatula Jemalog A17 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Medicago truncatula sunn-4 mutant Dr. Julia Frugoli, Clemson University N/A Chemicals, peptides, and recombinant proteins Protector RNase Inhibitor Sigma Aldrich SKU #3335399001 DAPI (40,6-diamidino-2-phenylindole) Sigma Aldrich PubChem Substance ID 24894305, SKU #D9542-5MG BD FACS Flow Sheath Buffer BD Biosciences catalog number: 342003 RNase inhibitor RibolockTM Thermo Fisher Scientific catalog number: EO0382 Critical commercial assays CYG germination pouches https://mega-international.com/tech-info/ N/A Polypap stands https://mega-international.com/tech-info/ N/A Humidity dome Alberta LTD Amazon Standard Identification Number: B089Q24D3N 40 mm cell strainer Greiner Bio-One Item No.: 542040 Miracloth Calbiochem Cat# 475855 Chromium Next GEM Chip G Single Cell Kit, 16 rxn 103 Genomics 1000127 MoClo Toolkit Addgene Kit #1000000044 Kulzer Technovit 7100 resin Electron Microscopy Sceinces 14653 Phusion Taq NEB M0530S Monarch DNA Plasmid Prep Kit NEB T1010S pENTR Directional TOPO vector Thermo Fisher K240020 Gatewa LR Clonas II Enzyme mix Thermo Fisher 11791100 10X Genomics Library Construction Kit 10X Genomics 1000190 10X Genomics Chromium Next GEM Single Cell 30 Kit v3.1, 4 Mutagenesis:Article Title: The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Sinorhizobium meliloti 1021 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Agrobacterium rhizogenes MSU440 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Biological samples Medicago truncatula Jemalog A17 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Medicago truncatula sunn-4 mutant Dr. Julia Frugoli, Clemson University N/A Chemicals, peptides, and recombinant proteins Protector RNase Inhibitor Sigma Aldrich SKU #3335399001 DAPI (40,6-diamidino-2-phenylindole) Sigma Aldrich PubChem Substance ID 24894305, SKU #D9542-5MG BD FACS Flow Sheath Buffer BD Biosciences catalog number: 342003 RNase inhibitor RibolockTM Thermo Fisher Scientific catalog number: EO0382 Critical commercial assays CYG germination pouches https://mega-international.com/tech-info/ N/A Polypap stands https://mega-international.com/tech-info/ N/A Humidity dome Alberta LTD Amazon Standard Identification Number: B089Q24D3N 40 mm cell strainer Greiner Bio-One Item No.: 542040 Miracloth Calbiochem Cat# 475855 Chromium Next GEM Chip G Single Cell Kit, 16 rxn 103 Genomics 1000127 MoClo Toolkit Addgene Kit #1000000044 Kulzer Technovit 7100 resin Electron Microscopy Sceinces 14653 Phusion Taq NEB M0530S Monarch DNA Plasmid Prep Kit NEB T1010S pENTR Directional TOPO vector Thermo Fisher K240020 Gatewa LR Clonas II Enzyme mix Thermo Fisher 11791100 10X Genomics Library Construction Kit 10X Genomics 1000190 10X Genomics Chromium Next GEM Single Cell 30 Kit v3.1, 4 Recombinant:Article Title: The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Sinorhizobium meliloti 1021 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Agrobacterium rhizogenes MSU440 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Biological samples Medicago truncatula Jemalog A17 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Medicago truncatula sunn-4 mutant Dr. Julia Frugoli, Clemson University N/A Chemicals, peptides, and recombinant proteins Protector RNase Inhibitor Sigma Aldrich SKU #3335399001 DAPI (40,6-diamidino-2-phenylindole) Sigma Aldrich PubChem Substance ID 24894305, SKU #D9542-5MG BD FACS Flow Sheath Buffer BD Biosciences catalog number: 342003 RNase inhibitor RibolockTM Thermo Fisher Scientific catalog number: EO0382 Critical commercial assays CYG germination pouches https://mega-international.com/tech-info/ N/A Polypap stands https://mega-international.com/tech-info/ N/A Humidity dome Alberta LTD Amazon Standard Identification Number: B089Q24D3N 40 mm cell strainer Greiner Bio-One Item No.: 542040 Miracloth Calbiochem Cat# 475855 Chromium Next GEM Chip G Single Cell Kit, 16 rxn 103 Genomics 1000127 MoClo Toolkit Addgene Kit #1000000044 Kulzer Technovit 7100 resin Electron Microscopy Sceinces 14653 Phusion Taq NEB M0530S Monarch DNA Plasmid Prep Kit NEB T1010S pENTR Directional TOPO vector Thermo Fisher K240020 Gatewa LR Clonas II Enzyme mix Thermo Fisher 11791100 10X Genomics Library Construction Kit 10X Genomics 1000190 10X Genomics Chromium Next GEM Single Cell 30 Kit v3.1, 4 FACS:Article Title: The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Sinorhizobium meliloti 1021 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Agrobacterium rhizogenes MSU440 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Biological samples Medicago truncatula Jemalog A17 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Medicago truncatula sunn-4 mutant Dr. Julia Frugoli, Clemson University N/A Chemicals, peptides, and recombinant proteins Protector RNase Inhibitor Sigma Aldrich SKU #3335399001 DAPI (40,6-diamidino-2-phenylindole) Sigma Aldrich PubChem Substance ID 24894305, SKU #D9542-5MG BD FACS Flow Sheath Buffer BD Biosciences catalog number: 342003 RNase inhibitor RibolockTM Thermo Fisher Scientific catalog number: EO0382 Critical commercial assays CYG germination pouches https://mega-international.com/tech-info/ N/A Polypap stands https://mega-international.com/tech-info/ N/A Humidity dome Alberta LTD Amazon Standard Identification Number: B089Q24D3N 40 mm cell strainer Greiner Bio-One Item No.: 542040 Miracloth Calbiochem Cat# 475855 Chromium Next GEM Chip G Single Cell Kit, 16 rxn 103 Genomics 1000127 MoClo Toolkit Addgene Kit #1000000044 Kulzer Technovit 7100 resin Electron Microscopy Sceinces 14653 Phusion Taq NEB M0530S Monarch DNA Plasmid Prep Kit NEB T1010S pENTR Directional TOPO vector Thermo Fisher K240020 Gatewa LR Clonas II Enzyme mix Thermo Fisher 11791100 10X Genomics Library Construction Kit 10X Genomics 1000190 10X Genomics Chromium Next GEM Single Cell 30 Kit v3.1, 4 Single Cell:Article Title: The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Sinorhizobium meliloti 1021 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Agrobacterium rhizogenes MSU440 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Biological samples Medicago truncatula Jemalog A17 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Medicago truncatula sunn-4 mutant Dr. Julia Frugoli, Clemson University N/A Chemicals, peptides, and recombinant proteins Protector RNase Inhibitor Sigma Aldrich SKU #3335399001 DAPI (40,6-diamidino-2-phenylindole) Sigma Aldrich PubChem Substance ID 24894305, SKU #D9542-5MG BD FACS Flow Sheath Buffer BD Biosciences catalog number: 342003 RNase inhibitor RibolockTM Thermo Fisher Scientific catalog number: EO0382 Critical commercial assays CYG germination pouches https://mega-international.com/tech-info/ N/A Polypap stands https://mega-international.com/tech-info/ N/A Humidity dome Alberta LTD Amazon Standard Identification Number: B089Q24D3N 40 mm cell strainer Greiner Bio-One Item No.: 542040 Miracloth Calbiochem Cat# 475855 Chromium Next GEM Chip G Single Cell Kit, 16 rxn 103 Genomics 1000127 MoClo Toolkit Addgene Kit #1000000044 Kulzer Technovit 7100 resin Electron Microscopy Sceinces 14653 Phusion Taq NEB M0530S Monarch DNA Plasmid Prep Kit NEB T1010S pENTR Directional TOPO vector Thermo Fisher K240020 Gatewa LR Clonas II Enzyme mix Thermo Fisher 11791100 10X Genomics Library Construction Kit 10X Genomics 1000190 10X Genomics Chromium Next GEM Single Cell 30 Kit v3.1, 4 Electron Microscopy:Article Title: The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Sinorhizobium meliloti 1021 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Agrobacterium rhizogenes MSU440 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Biological samples Medicago truncatula Jemalog A17 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Medicago truncatula sunn-4 mutant Dr. Julia Frugoli, Clemson University N/A Chemicals, peptides, and recombinant proteins Protector RNase Inhibitor Sigma Aldrich SKU #3335399001 DAPI (40,6-diamidino-2-phenylindole) Sigma Aldrich PubChem Substance ID 24894305, SKU #D9542-5MG BD FACS Flow Sheath Buffer BD Biosciences catalog number: 342003 RNase inhibitor RibolockTM Thermo Fisher Scientific catalog number: EO0382 Critical commercial assays CYG germination pouches https://mega-international.com/tech-info/ N/A Polypap stands https://mega-international.com/tech-info/ N/A Humidity dome Alberta LTD Amazon Standard Identification Number: B089Q24D3N 40 mm cell strainer Greiner Bio-One Item No.: 542040 Miracloth Calbiochem Cat# 475855 Chromium Next GEM Chip G Single Cell Kit, 16 rxn 103 Genomics 1000127 MoClo Toolkit Addgene Kit #1000000044 Kulzer Technovit 7100 resin Electron Microscopy Sceinces 14653 Phusion Taq NEB M0530S Monarch DNA Plasmid Prep Kit NEB T1010S pENTR Directional TOPO vector Thermo Fisher K240020 Gatewa LR Clonas II Enzyme mix Thermo Fisher 11791100 10X Genomics Library Construction Kit 10X Genomics 1000190 10X Genomics Chromium Next GEM Single Cell 30 Kit v3.1, 4 Gel Extraction:Article Title: The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Sinorhizobium meliloti 1021 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Agrobacterium rhizogenes MSU440 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Biological samples Medicago truncatula Jemalog A17 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Medicago truncatula sunn-4 mutant Dr. Julia Frugoli, Clemson University N/A Chemicals, peptides, and recombinant proteins Protector RNase Inhibitor Sigma Aldrich SKU #3335399001 DAPI (40,6-diamidino-2-phenylindole) Sigma Aldrich PubChem Substance ID 24894305, SKU #D9542-5MG BD FACS Flow Sheath Buffer BD Biosciences catalog number: 342003 RNase inhibitor RibolockTM Thermo Fisher Scientific catalog number: EO0382 Critical commercial assays CYG germination pouches https://mega-international.com/tech-info/ N/A Polypap stands https://mega-international.com/tech-info/ N/A Humidity dome Alberta LTD Amazon Standard Identification Number: B089Q24D3N 40 mm cell strainer Greiner Bio-One Item No.: 542040 Miracloth Calbiochem Cat# 475855 Chromium Next GEM Chip G Single Cell Kit, 16 rxn 103 Genomics 1000127 MoClo Toolkit Addgene Kit #1000000044 Kulzer Technovit 7100 resin Electron Microscopy Sceinces 14653 Phusion Taq NEB M0530S Monarch DNA Plasmid Prep Kit NEB T1010S pENTR Directional TOPO vector Thermo Fisher K240020 Gatewa LR Clonas II Enzyme mix Thermo Fisher 11791100 10X Genomics Library Construction Kit 10X Genomics 1000190 10X Genomics Chromium Next GEM Single Cell 30 Kit v3.1, 4 Generated:Article Title: The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Sinorhizobium meliloti 1021 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Agrobacterium rhizogenes MSU440 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Biological samples Medicago truncatula Jemalog A17 Dr. Jean Michel-Ané, University of Wisconsin-Madison N/A Medicago truncatula sunn-4 mutant Dr. Julia Frugoli, Clemson University N/A Chemicals, peptides, and recombinant proteins Protector RNase Inhibitor Sigma Aldrich SKU #3335399001 DAPI (40,6-diamidino-2-phenylindole) Sigma Aldrich PubChem Substance ID 24894305, SKU #D9542-5MG BD FACS Flow Sheath Buffer BD Biosciences catalog number: 342003 RNase inhibitor RibolockTM Thermo Fisher Scientific catalog number: EO0382 Critical commercial assays CYG germination pouches https://mega-international.com/tech-info/ N/A Polypap stands https://mega-international.com/tech-info/ N/A Humidity dome Alberta LTD Amazon Standard Identification Number: B089Q24D3N 40 mm cell strainer Greiner Bio-One Item No.: 542040 Miracloth Calbiochem Cat# 475855 Chromium Next GEM Chip G Single Cell Kit, 16 rxn 103 Genomics 1000127 MoClo Toolkit Addgene Kit #1000000044 Kulzer Technovit 7100 resin Electron Microscopy Sceinces 14653 Phusion Taq NEB M0530S Monarch DNA Plasmid Prep Kit NEB T1010S pENTR Directional TOPO vector Thermo Fisher K240020 Gatewa LR Clonas II Enzyme mix Thermo Fisher 11791100 10X Genomics Library Construction Kit 10X Genomics 1000190 10X Genomics Chromium Next GEM Single Cell 30 Kit v3.1, 4 Article Title: AAV-mediated BDNF and GAS6 muscle delivery delays disease onset in SOD1 G93A ALS mice. Article Snippet: .. MATERIALS AND METHODS Vector plasmid construction pAAV plasmidswere generated using the pAAV-CMV-teLuc backbonewhichwas constructed by substituting the eGFP gene from the pAAV-CMV-eGFP (Addgene Cat. 67634) with a teLuc gene PCR fragment, utilizing Derivative Assay:Article Title: Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging <i>In Vivo</i> Article Snippet: Plasmids derived PCR Template PCR Oligos Inserted Vectors Note pBAD-QLuc pcDNA3-LumiLuc (Addgene # 126622) pBAD_F_QLuc, pBAD_R_QLuc pBAD/His B 5 rounds of EP-PCR & directed evolution pcDNA3-QLuc (Addgene # 183260) pBAD-QLuc pcDNA3_F_QLuc, pcDNA3_R_QLuc pcDNA3 For mammalian expression pAAV-TBGNLuc pcDNA3-NLuc pAAV_F_NLuc, pAAV_R_NLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGteLuc pcDNA3-teLuc (Addgene # 100026) pAAV_F_NLuc, pAAV_R_teLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGLumiLuc pcDNA3-LumiLuc (Addgene # 126622) pAAV_F _QLuc, pAAV_R_QLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGQLuc pBAD-QLuc pAAV_F _QLuc, pAAV_R_QLuc pAAV-TBG (Addgene # 105535) For AAV packaging pET-scFv-QLuc (Addgene # 183261) pBAD-QLuc and a custom synthetic scFv gene fragment pET_F1_scFvQLuc pET_R1_scFvAnt pET_F2_scFvAnt pET_R2_scFvAnt pET28a For bacterial expression in SHuffle T7 Cells pET-scFvAntares pNCS-Antares (Addgene #74279) and a custom synthetic scFv gene fragment pET_F1_scFvQLuc pET_R1_scFvAnt pET_F2_scFvAnt pET_R2_scFvAnt pET28a For bacterial expression in SHuffle T7 Cells pLX208-CMVAkaLuc pcDNA3-AkaLuc Plx208-F-Akluc, Plx208-R-Akluc pLX208-CMV (Addgene # 153007) For lentiviral packaging S14 Scheme S1. .. Plasmids derived PCR Template PCR Oligos Inserted Vectors Note pBAD-QLuc pcDNA3-LumiLuc (Addgene # 126622) pBAD_F_QLuc, pBAD_R_QLuc pBAD/His B 5 rounds of EP-PCR & directed evolution pcDNA3-QLuc (Addgene # 183260) pBAD-QLuc pcDNA3_F_QLuc, pcDNA3_R_QLuc pcDNA3 For mammalian expression pAAV-TBGNLuc pcDNA3-NLuc pAAV_F_NLuc, pAAV_R_NLuc pAAV-TBG (Addgene # 105535) For Polymerase Chain Reaction:Article Title: Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging <i>In Vivo</i> Article Snippet: Plasmids derived PCR Template PCR Oligos Inserted Vectors Note pBAD-QLuc pcDNA3-LumiLuc (Addgene # 126622) pBAD_F_QLuc, pBAD_R_QLuc pBAD/His B 5 rounds of EP-PCR & directed evolution pcDNA3-QLuc (Addgene # 183260) pBAD-QLuc pcDNA3_F_QLuc, pcDNA3_R_QLuc pcDNA3 For mammalian expression pAAV-TBGNLuc pcDNA3-NLuc pAAV_F_NLuc, pAAV_R_NLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGteLuc pcDNA3-teLuc (Addgene # 100026) pAAV_F_NLuc, pAAV_R_teLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGLumiLuc pcDNA3-LumiLuc (Addgene # 126622) pAAV_F _QLuc, pAAV_R_QLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGQLuc pBAD-QLuc pAAV_F _QLuc, pAAV_R_QLuc pAAV-TBG (Addgene # 105535) For AAV packaging pET-scFv-QLuc (Addgene # 183261) pBAD-QLuc and a custom synthetic scFv gene fragment pET_F1_scFvQLuc pET_R1_scFvAnt pET_F2_scFvAnt pET_R2_scFvAnt pET28a For bacterial expression in SHuffle T7 Cells pET-scFvAntares pNCS-Antares (Addgene #74279) and a custom synthetic scFv gene fragment pET_F1_scFvQLuc pET_R1_scFvAnt pET_F2_scFvAnt pET_R2_scFvAnt pET28a For bacterial expression in SHuffle T7 Cells pLX208-CMVAkaLuc pcDNA3-AkaLuc Plx208-F-Akluc, Plx208-R-Akluc pLX208-CMV (Addgene # 153007) For lentiviral packaging S14 Scheme S1. .. Plasmids derived PCR Template PCR Oligos Inserted Vectors Note pBAD-QLuc pcDNA3-LumiLuc (Addgene # 126622) pBAD_F_QLuc, pBAD_R_QLuc pBAD/His B 5 rounds of EP-PCR & directed evolution pcDNA3-QLuc (Addgene # 183260) pBAD-QLuc pcDNA3_F_QLuc, pcDNA3_R_QLuc pcDNA3 For mammalian expression pAAV-TBGNLuc pcDNA3-NLuc pAAV_F_NLuc, pAAV_R_NLuc pAAV-TBG (Addgene # 105535) For Article Title: AAV-mediated BDNF and GAS6 muscle delivery delays disease onset in SOD1 G93A ALS mice. Article Snippet: .. MATERIALS AND METHODS Vector plasmid construction pAAV plasmidswere generated using the pAAV-CMV-teLuc backbonewhichwas constructed by substituting the eGFP gene from the pAAV-CMV-eGFP (Addgene Cat. 67634) with a teLuc gene PCR fragment, utilizing Expressing:Article Title: Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging <i>In Vivo</i> Article Snippet: Plasmids derived PCR Template PCR Oligos Inserted Vectors Note pBAD-QLuc pcDNA3-LumiLuc (Addgene # 126622) pBAD_F_QLuc, pBAD_R_QLuc pBAD/His B 5 rounds of EP-PCR & directed evolution pcDNA3-QLuc (Addgene # 183260) pBAD-QLuc pcDNA3_F_QLuc, pcDNA3_R_QLuc pcDNA3 For mammalian expression pAAV-TBGNLuc pcDNA3-NLuc pAAV_F_NLuc, pAAV_R_NLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGteLuc pcDNA3-teLuc (Addgene # 100026) pAAV_F_NLuc, pAAV_R_teLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGLumiLuc pcDNA3-LumiLuc (Addgene # 126622) pAAV_F _QLuc, pAAV_R_QLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGQLuc pBAD-QLuc pAAV_F _QLuc, pAAV_R_QLuc pAAV-TBG (Addgene # 105535) For AAV packaging pET-scFv-QLuc (Addgene # 183261) pBAD-QLuc and a custom synthetic scFv gene fragment pET_F1_scFvQLuc pET_R1_scFvAnt pET_F2_scFvAnt pET_R2_scFvAnt pET28a For bacterial expression in SHuffle T7 Cells pET-scFvAntares pNCS-Antares (Addgene #74279) and a custom synthetic scFv gene fragment pET_F1_scFvQLuc pET_R1_scFvAnt pET_F2_scFvAnt pET_R2_scFvAnt pET28a For bacterial expression in SHuffle T7 Cells pLX208-CMVAkaLuc pcDNA3-AkaLuc Plx208-F-Akluc, Plx208-R-Akluc pLX208-CMV (Addgene # 153007) For lentiviral packaging S14 Scheme S1. .. Plasmids derived PCR Template PCR Oligos Inserted Vectors Note pBAD-QLuc pcDNA3-LumiLuc (Addgene # 126622) pBAD_F_QLuc, pBAD_R_QLuc pBAD/His B 5 rounds of EP-PCR & directed evolution pcDNA3-QLuc (Addgene # 183260) pBAD-QLuc pcDNA3_F_QLuc, pcDNA3_R_QLuc pcDNA3 For mammalian expression pAAV-TBGNLuc pcDNA3-NLuc pAAV_F_NLuc, pAAV_R_NLuc pAAV-TBG (Addgene # 105535) For Bioprocessing:Article Title: Engineered Amber-Emitting Nano Luciferase and Its Use for Immunobioluminescence Imaging <i>In Vivo</i> Article Snippet: Plasmids derived PCR Template PCR Oligos Inserted Vectors Note pBAD-QLuc pcDNA3-LumiLuc (Addgene # 126622) pBAD_F_QLuc, pBAD_R_QLuc pBAD/His B 5 rounds of EP-PCR & directed evolution pcDNA3-QLuc (Addgene # 183260) pBAD-QLuc pcDNA3_F_QLuc, pcDNA3_R_QLuc pcDNA3 For mammalian expression pAAV-TBGNLuc pcDNA3-NLuc pAAV_F_NLuc, pAAV_R_NLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGteLuc pcDNA3-teLuc (Addgene # 100026) pAAV_F_NLuc, pAAV_R_teLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGLumiLuc pcDNA3-LumiLuc (Addgene # 126622) pAAV_F _QLuc, pAAV_R_QLuc pAAV-TBG (Addgene # 105535) For AAV packaging pAAV-TBGQLuc pBAD-QLuc pAAV_F _QLuc, pAAV_R_QLuc pAAV-TBG (Addgene # 105535) For AAV packaging pET-scFv-QLuc (Addgene # 183261) pBAD-QLuc and a custom synthetic scFv gene fragment pET_F1_scFvQLuc pET_R1_scFvAnt pET_F2_scFvAnt pET_R2_scFvAnt pET28a For bacterial expression in SHuffle T7 Cells pET-scFvAntares pNCS-Antares (Addgene #74279) and a custom synthetic scFv gene fragment pET_F1_scFvQLuc pET_R1_scFvAnt pET_F2_scFvAnt pET_R2_scFvAnt pET28a For bacterial expression in SHuffle T7 Cells pLX208-CMVAkaLuc pcDNA3-AkaLuc Plx208-F-Akluc, Plx208-R-Akluc pLX208-CMV (Addgene # 153007) For lentiviral packaging S14 Scheme S1. .. Plasmids derived PCR Template PCR Oligos Inserted Vectors Note pBAD-QLuc pcDNA3-LumiLuc (Addgene # 126622) pBAD_F_QLuc, pBAD_R_QLuc pBAD/His B 5 rounds of EP-PCR & directed evolution pcDNA3-QLuc (Addgene # 183260) pBAD-QLuc pcDNA3_F_QLuc, pcDNA3_R_QLuc pcDNA3 For mammalian expression pAAV-TBGNLuc pcDNA3-NLuc pAAV_F_NLuc, pAAV_R_NLuc pAAV-TBG (Addgene # 105535) For Construct:Article Title: AAV-mediated BDNF and GAS6 muscle delivery delays disease onset in SOD1 G93A ALS mice. Article Snippet: .. MATERIALS AND METHODS Vector plasmid construction pAAV plasmidswere generated using the pAAV-CMV-teLuc backbonewhichwas constructed by substituting the eGFP gene from the pAAV-CMV-eGFP (Addgene Cat. 67634) with a teLuc gene PCR fragment, utilizing |