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plasmids coding for shrna targeting human foxo1 and scrambled shrna as negative control  (Ribobio co)

 
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    Ribobio co plasmids coding for shrna targeting human foxo1 and scrambled shrna as negative control
    Plasmids Coding For Shrna Targeting Human Foxo1 And Scrambled Shrna As Negative Control, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+coding+shrna+control/foxo1+sirna/pmc06096113-151-9-17
    Average 90 stars, based on 1 article reviews
    plasmids coding for shrna targeting human foxo1 and scrambled shrna as negative control - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Tumor-derived exosomal miR-1247-3p promotes angiogenesis in bladder cancer by targeting FOXO1.
    Article Snippet: The miR-1247-3p inhibitor and si-FOXO1, and the inhibitor NC and si-NC were synthesized from RiboBio Co., Ltd (Guangzhou, China).

    Article Title: LncRNA DANCR deficiency promotes high glucose-induced endothelial to mesenchymal transition in cardiac microvascular cells via the FoxO1/DDAH1/ADMA signaling pathway.
    Article Snippet: Cardiac fibrosis is the main pathological basis of diabetic cardiomyopathy (DCM), and endothelial-tomeschenymal transition (EndMT) is a key driver to cardiac fibrosis and plays an important role in the pathogenesis of DCM.. Asymmetric dimethylarginine (ADMA), a crucial pathologic factor in diabetes mellitus, is involved in organ fibrosis.. This study aims to evaluate underlying mechanisms of ADMA in DCM especially for EndMT under diabetic conditions.

    Article Title: EGF-driven EGFR/miR-27b-3p/FOXO1 promotes rat FSH synthesis and secretion.
    Article Snippet: Folliclestimulating hormone (FSH) is a heterodimeric glycoprotein hormone composed of α and β subunits that are secreted by gonadotropin cells located in the anterior pituitary.. FSH is involved in regulating follicle development,1 oogenesis,2 and spermatogenesis.3 It has also been linked to reproductive disorders such as polycystic ovary syndrome4 and infertility.5 Moreover, outside the reproductive system, FSH plays important roles in biological activities Received: 26 September 2023 | Revised: 4 January 2024 | Accepted: 23 January 2024 DOI: 10.1096/fj.202301970R

    Negative Control:

    Article Title: FOXO1 regulates RUNX2 ubiquitination through SMURF2 in calcific aortic valve disease.
    Article Snippet: .. siRNAs targeting FOXO1, SMURF2 and the negative control siRNA (si-NC) with no definite target were designed and synthesized by RiboBio. (Guangzhou, China). ..

    Synthesized:

    Article Title: FOXO1 regulates RUNX2 ubiquitination through SMURF2 in calcific aortic valve disease.
    Article Snippet: .. siRNAs targeting FOXO1, SMURF2 and the negative control siRNA (si-NC) with no definite target were designed and synthesized by RiboBio. (Guangzhou, China). ..

    Transfection:

    Article Title: The FOXO1/G6PC axis promotes gastric cancer progression and mediates 5-fluorouracil resistance by targeting the PI3K/AKT/mTOR signaling pathway.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 82160695; Projects of Science and Technology Department of Jilin Province, Grant/Award Number: 20210101422JC Abstract Gastric cancer (GC) is a prevalent malignancy of the digestive system.. Distant metastasis and chemotherapy resistance are the crucial obstacles to prognosis in GC.. Recent research has discovered that the glucose‐6‐phosphatase catalytic subunit (G6PC) plays an important role in tumor malignant development.

    Small Interfering RNA:

    Article Title: Ginkgolide A enhances FoxO1 expression and reduces endoplasmic reticulum stress to mitigate osteoarthritis in mice.
    Article Snippet: This study aimed to investigate the effects of Ginkgolide A (GA) on chondrocytes under oxidative stress and to elucidate its potential molecular mechanisms.. Using a destabilization of the medial meniscus (DMM) model in mice and an in vitro osteoarthritis (OA) model induced by tert-butyl hydroperoxide (TBHP) in chondrocytes, we validated the therapeutic efficacy and underlying mechanisms of GA.. Potential OA targets of GA were identified through network pharmacology, Gene Ontology (GO) analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.



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    Figure 3 | CYREN interaction with Ku in S and G2 inhibits cNHEJ. a, CYREN isoforms. b, Mean percentage of fused ends per metaphase. siNT, control non-targeting <t>siRNA;</t> siCY, CYREN-targeting siRNA. Error bars, s.e.m. ***P < 0.001, *P < 0.05; NS, not significant. One-way ANOVA, Sidak’s multiple comparison test. n, number of metaphases analysed. Experiment shown is representative of two biological replicates. c, Co-immunoprecipitation of CYREN-1–3×Flag with Ku70/80 in HEK293T cells. For gel source data, see Supplementary Fig. 1. d, Percentage of fusions ± upper and lower value of 95% confidence intervals, Wilson–Brown test. ***P < 0.001, **P < 0.01;
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    Image Search Results


    LAMZ induces Mef2c to enhance myogenesis and osteoblastogenesis. a mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells treated with calcium signal inhibitors together with LAMZ. b mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells infected with lentiviruses encoding sh Mef2c . c Representative immunocytofluorescence images of the C2C12 cells infected with lentiviruses encoding sh Mef2c and treated with LAMZ. Myosin heavy chain (red); nuclei (blue). Scale bar, 100 μm. d mRNA expression of Myog and Ppargc1a . e Representative ALP staining images of the MC3T3-E1 cells infected with lentiviruses encoding sh Mef2c and treated with LAMZ. f Representative images of Alizarin Red S staining of MC3T3-E1 cells. g mRNA expression of osteoblastic genes and Ppargc1a . h mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells treated with the PGC-1α inhibitor SR18292. Experiments were repeated 3 times with replicates of two wells. For the comparison of two groups, statistical analyses were carried out using Welch’s t test. For multiple comparisons, two–way ANOVA and Tukey’s multiple-comparison test were applied. The error bars show the mean ± s.e.m. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; N.S. not significant

    Journal: Bone Research

    Article Title: Simultaneous augmentation of muscle and bone by locomomimetism through calcium-PGC-1α signaling

    doi: 10.1038/s41413-022-00225-w

    Figure Lengend Snippet: LAMZ induces Mef2c to enhance myogenesis and osteoblastogenesis. a mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells treated with calcium signal inhibitors together with LAMZ. b mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells infected with lentiviruses encoding sh Mef2c . c Representative immunocytofluorescence images of the C2C12 cells infected with lentiviruses encoding sh Mef2c and treated with LAMZ. Myosin heavy chain (red); nuclei (blue). Scale bar, 100 μm. d mRNA expression of Myog and Ppargc1a . e Representative ALP staining images of the MC3T3-E1 cells infected with lentiviruses encoding sh Mef2c and treated with LAMZ. f Representative images of Alizarin Red S staining of MC3T3-E1 cells. g mRNA expression of osteoblastic genes and Ppargc1a . h mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells treated with the PGC-1α inhibitor SR18292. Experiments were repeated 3 times with replicates of two wells. For the comparison of two groups, statistical analyses were carried out using Welch’s t test. For multiple comparisons, two–way ANOVA and Tukey’s multiple-comparison test were applied. The error bars show the mean ± s.e.m. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; N.S. not significant

    Article Snippet: The plasmids for producing lentivirus that code shRNAs targeting Mef2c and gfp (control) were purchased from Sigma-Aldrich.

    Techniques: Expressing, Infection, Staining

    Figure 3 | CYREN interaction with Ku in S and G2 inhibits cNHEJ. a, CYREN isoforms. b, Mean percentage of fused ends per metaphase. siNT, control non-targeting siRNA; siCY, CYREN-targeting siRNA. Error bars, s.e.m. ***P < 0.001, *P < 0.05; NS, not significant. One-way ANOVA, Sidak’s multiple comparison test. n, number of metaphases analysed. Experiment shown is representative of two biological replicates. c, Co-immunoprecipitation of CYREN-1–3×Flag with Ku70/80 in HEK293T cells. For gel source data, see Supplementary Fig. 1. d, Percentage of fusions ± upper and lower value of 95% confidence intervals, Wilson–Brown test. ***P < 0.001, **P < 0.01;

    Journal: Nature

    Article Title: Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN.

    doi: 10.1038/nature24023

    Figure Lengend Snippet: Figure 3 | CYREN interaction with Ku in S and G2 inhibits cNHEJ. a, CYREN isoforms. b, Mean percentage of fused ends per metaphase. siNT, control non-targeting siRNA; siCY, CYREN-targeting siRNA. Error bars, s.e.m. ***P < 0.001, *P < 0.05; NS, not significant. One-way ANOVA, Sidak’s multiple comparison test. n, number of metaphases analysed. Experiment shown is representative of two biological replicates. c, Co-immunoprecipitation of CYREN-1–3×Flag with Ku70/80 in HEK293T cells. For gel source data, see Supplementary Fig. 1. d, Percentage of fusions ± upper and lower value of 95% confidence intervals, Wilson–Brown test. ***P < 0.001, **P < 0.01;

    Article Snippet: The non-coding control shRNA in pLKO.1 was obtained from D. Sabatini via Addgene (Plasmid 186432).

    Techniques: Control, Comparison, Immunoprecipitation