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EZBiolab Inc non-target shrna control plasmid
Non Target Shrna Control Plasmid, supplied by EZBiolab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+coding+shrna+control/non+target+shrna+control+plasmid/pmc04021846-166-0-28
Average 90 stars, based on 1 article reviews
non-target shrna control plasmid - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Expressing:

Article Title: A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis
Article Snippet: .. The Flag-tagged expression constructs for mouse and human Smaug2 and Smaug1 and mouse Nanos1, Nanos 2, and Nanos3 and human Nanos1 were obtained from OriGene. shRNA vectors were obtained from EZBiolab and had the following sequences: Smaug2 shRNA-1 5′-GAG GAG AAC ATC ACC AGT TAC T-3′, Smaug2 shRNA-2 5′-GGG CTG GAA TGA GTG TGA ACAT-3′, and Nanos1 shRNA-1 5′-GCACATACCATCAAGTATTGCT-3′. ..

Construct:

Article Title: A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis
Article Snippet: .. The Flag-tagged expression constructs for mouse and human Smaug2 and Smaug1 and mouse Nanos1, Nanos 2, and Nanos3 and human Nanos1 were obtained from OriGene. shRNA vectors were obtained from EZBiolab and had the following sequences: Smaug2 shRNA-1 5′-GAG GAG AAC ATC ACC AGT TAC T-3′, Smaug2 shRNA-2 5′-GGG CTG GAA TGA GTG TGA ACAT-3′, and Nanos1 shRNA-1 5′-GCACATACCATCAAGTATTGCT-3′. ..

shRNA:

Article Title: A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis
Article Snippet: .. The Flag-tagged expression constructs for mouse and human Smaug2 and Smaug1 and mouse Nanos1, Nanos 2, and Nanos3 and human Nanos1 were obtained from OriGene. shRNA vectors were obtained from EZBiolab and had the following sequences: Smaug2 shRNA-1 5′-GAG GAG AAC ATC ACC AGT TAC T-3′, Smaug2 shRNA-2 5′-GGG CTG GAA TGA GTG TGA ACAT-3′, and Nanos1 shRNA-1 5′-GCACATACCATCAAGTATTGCT-3′. ..

Article Title: Distribution and function of TrkB receptors in the developing brain of the opossum Monodelphis domestica.
Article Snippet: The expression, development pattern, spatiotemporal distribution, and function of TrkB receptors were investigated during the postnatal brain development of the opossum.. Full-length TrkB receptor expression was detectable in the newborn opossum, whereas three different short forms that are expressed in the adult brain were almost undetectable in the newborn opossum brain.. The highest level of full-length TrkB receptor expression was observed at P35, which corresponds to the time of eye opening.

other:

Article Title: SELECTIVE UP-REGULATION OF INTERLEUKIN-8 BY HUMAN RHABDOMYOSARCOMAS IN RESPONSE TO HYPOXIA: THERAPEUTIC IMPLICATIONS
Article Snippet: Non-target shRNA control plasmid contained a harpin insert 5′ – TTC TCC GAA CGT GTC ACG TTT CAA GAG AAC GTG ACA CGT TCG GAG AA – 3′. (EZBiolab Inc., Westfield, IN) RMS cells were transfected using lipofectamine 2000 (Invitrogen, Carlsbad, CA).

Article Title: Transglutaminase 2 cross-linking activity is linked to invadopodia formation and cartilage breakdown in arthritis
Article Snippet: GFP-lentiviral shRNAs targeting rat TG2 and control (scrambled) shRNA plasmids were from ezBiolab (Carmel, IN, USA).

Article Title: Snail Is a Critical Mediator of Invadosome Formation and Joint Degradation in Arthritis.
Article Snippet: GFP-lentiviral shRNA targeting rat TG2 and control (scrambled) shRNA plasmids were from EzBiolab (Carmel, IN).

Control:

Article Title: Distribution and function of TrkB receptors in the developing brain of the opossum Monodelphis domestica.
Article Snippet: The expression, development pattern, spatiotemporal distribution, and function of TrkB receptors were investigated during the postnatal brain development of the opossum.. Full-length TrkB receptor expression was detectable in the newborn opossum, whereas three different short forms that are expressed in the adult brain were almost undetectable in the newborn opossum brain.. The highest level of full-length TrkB receptor expression was observed at P35, which corresponds to the time of eye opening.



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Figure 3 | CYREN interaction with Ku in S and G2 inhibits cNHEJ. a, CYREN isoforms. b, Mean percentage of fused ends per metaphase. siNT, control non-targeting <t>siRNA;</t> siCY, CYREN-targeting siRNA. Error bars, s.e.m. ***P < 0.001, *P < 0.05; NS, not significant. One-way ANOVA, Sidak’s multiple comparison test. n, number of metaphases analysed. Experiment shown is representative of two biological replicates. c, Co-immunoprecipitation of CYREN-1–3×Flag with Ku70/80 in HEK293T cells. For gel source data, see Supplementary Fig. 1. d, Percentage of fusions ± upper and lower value of 95% confidence intervals, Wilson–Brown test. ***P < 0.001, **P < 0.01;
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Figure 3 | CYREN interaction with Ku in S and G2 inhibits cNHEJ. a, CYREN isoforms. b, Mean percentage of fused ends per metaphase. siNT, control non-targeting <t>siRNA;</t> siCY, CYREN-targeting siRNA. Error bars, s.e.m. ***P < 0.001, *P < 0.05; NS, not significant. One-way ANOVA, Sidak’s multiple comparison test. n, number of metaphases analysed. Experiment shown is representative of two biological replicates. c, Co-immunoprecipitation of CYREN-1–3×Flag with Ku70/80 in HEK293T cells. For gel source data, see Supplementary Fig. 1. d, Percentage of fusions ± upper and lower value of 95% confidence intervals, Wilson–Brown test. ***P < 0.001, **P < 0.01;
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Image Search Results


LAMZ induces Mef2c to enhance myogenesis and osteoblastogenesis. a mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells treated with calcium signal inhibitors together with LAMZ. b mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells infected with lentiviruses encoding sh Mef2c . c Representative immunocytofluorescence images of the C2C12 cells infected with lentiviruses encoding sh Mef2c and treated with LAMZ. Myosin heavy chain (red); nuclei (blue). Scale bar, 100 μm. d mRNA expression of Myog and Ppargc1a . e Representative ALP staining images of the MC3T3-E1 cells infected with lentiviruses encoding sh Mef2c and treated with LAMZ. f Representative images of Alizarin Red S staining of MC3T3-E1 cells. g mRNA expression of osteoblastic genes and Ppargc1a . h mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells treated with the PGC-1α inhibitor SR18292. Experiments were repeated 3 times with replicates of two wells. For the comparison of two groups, statistical analyses were carried out using Welch’s t test. For multiple comparisons, two–way ANOVA and Tukey’s multiple-comparison test were applied. The error bars show the mean ± s.e.m. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; N.S. not significant

Journal: Bone Research

Article Title: Simultaneous augmentation of muscle and bone by locomomimetism through calcium-PGC-1α signaling

doi: 10.1038/s41413-022-00225-w

Figure Lengend Snippet: LAMZ induces Mef2c to enhance myogenesis and osteoblastogenesis. a mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells treated with calcium signal inhibitors together with LAMZ. b mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells infected with lentiviruses encoding sh Mef2c . c Representative immunocytofluorescence images of the C2C12 cells infected with lentiviruses encoding sh Mef2c and treated with LAMZ. Myosin heavy chain (red); nuclei (blue). Scale bar, 100 μm. d mRNA expression of Myog and Ppargc1a . e Representative ALP staining images of the MC3T3-E1 cells infected with lentiviruses encoding sh Mef2c and treated with LAMZ. f Representative images of Alizarin Red S staining of MC3T3-E1 cells. g mRNA expression of osteoblastic genes and Ppargc1a . h mRNA expression of Mef2c in the C2C12 cells and MC3T3-E1 cells treated with the PGC-1α inhibitor SR18292. Experiments were repeated 3 times with replicates of two wells. For the comparison of two groups, statistical analyses were carried out using Welch’s t test. For multiple comparisons, two–way ANOVA and Tukey’s multiple-comparison test were applied. The error bars show the mean ± s.e.m. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; N.S. not significant

Article Snippet: The plasmids for producing lentivirus that code shRNAs targeting Mef2c and gfp (control) were purchased from Sigma-Aldrich.

Techniques: Expressing, Infection, Staining

Figure 3 | CYREN interaction with Ku in S and G2 inhibits cNHEJ. a, CYREN isoforms. b, Mean percentage of fused ends per metaphase. siNT, control non-targeting siRNA; siCY, CYREN-targeting siRNA. Error bars, s.e.m. ***P < 0.001, *P < 0.05; NS, not significant. One-way ANOVA, Sidak’s multiple comparison test. n, number of metaphases analysed. Experiment shown is representative of two biological replicates. c, Co-immunoprecipitation of CYREN-1–3×Flag with Ku70/80 in HEK293T cells. For gel source data, see Supplementary Fig. 1. d, Percentage of fusions ± upper and lower value of 95% confidence intervals, Wilson–Brown test. ***P < 0.001, **P < 0.01;

Journal: Nature

Article Title: Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN.

doi: 10.1038/nature24023

Figure Lengend Snippet: Figure 3 | CYREN interaction with Ku in S and G2 inhibits cNHEJ. a, CYREN isoforms. b, Mean percentage of fused ends per metaphase. siNT, control non-targeting siRNA; siCY, CYREN-targeting siRNA. Error bars, s.e.m. ***P < 0.001, *P < 0.05; NS, not significant. One-way ANOVA, Sidak’s multiple comparison test. n, number of metaphases analysed. Experiment shown is representative of two biological replicates. c, Co-immunoprecipitation of CYREN-1–3×Flag with Ku70/80 in HEK293T cells. For gel source data, see Supplementary Fig. 1. d, Percentage of fusions ± upper and lower value of 95% confidence intervals, Wilson–Brown test. ***P < 0.001, **P < 0.01;

Article Snippet: The non-coding control shRNA in pLKO.1 was obtained from D. Sabatini via Addgene (Plasmid 186432).

Techniques: Control, Comparison, Immunoprecipitation