plko 1 puro constructs (Addgene inc)
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Addgene inc
plko 1 puro constructs
Plko 1 Puro Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pLKO%2E1+puro+(Plasmid+%238453)/pmc13010945-62-9-16
Average 96 stars, based on 1591 article reviews
Plko 1 Puro Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+constructs/pLKO%2E1+puro+(Plasmid+%238453)/pmc13010945-62-9-16
Average 96 stars, based on 1591 article reviews
plko 1 puro constructs - by Bioz Stars,
2026-10
96/100 stars
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shRNA:Article Title: Prohibitin 2 mediates the mitochondrial protective effect of the whey-derived tetrapeptide Gly-Thr-Trp-Tyr. Article Snippet: .. These oligonucleotides were phosphorylated, annealed, and inserted into the Article Title: DNA replication stress redistributes the histone methyltransferase G9a and activates lineage-specific gene expression Article Snippet: .. HRI knockdown was performed using shRNA constructs (TRCN0000010229 and TRCN0000010231 from the Broad Genome Perturbation Platform) expressed from a Article Title: CircACVR2A(2-4) regulates stem cell pluripotency by uncoupling CDK-activating kinase functions. Article Snippet: .. Short hairpin RNA and circRNA overexpression constructs All human RNA-targeting shRNAs were cloned into Article Title: Fanconi anemia pathway disruption drives lomustine sensitivity in glioma. Article Snippet: 209 Apoptosis Analysis 210 Apoptosis signal under CCNU and PIP-199 treatment in different cell lines was assessed with the BD 211 Annexin V/PI detection Kit (BD, Franklin Lakes, NJ, USA) according to the manufacturer’s protocol as 212 previously described.21 Further information can be found in the Supplementary information. .. 213 Primary glioblastoma cultures 214 Primary cultures were generated and treated as previously described and outlined in the 215 Supplementary Methods.19,21 216 shRNA knockdown human cell lines (lentivirus) 217 We introduced shRNA sequences (Supplementary Table ST4) into Plasmid Preparation:Article Title: Prohibitin 2 mediates the mitochondrial protective effect of the whey-derived tetrapeptide Gly-Thr-Trp-Tyr. Article Snippet: .. These oligonucleotides were phosphorylated, annealed, and inserted into the Article Title: LAMTOR2 Reprograms Energy Metabolism to Sustain Hepatocellular Carcinoma Cell Survival Article Snippet: Hepatocellular carcinoma (HCC) is characterized by profound metabolic reprogramming to support rapid proliferation and survival under nutrient stress.. However, the central regulatory hubs that coordinate these diverse metabolic programs remain poorly understood.. Here, we identify LAMTOR2 as a critical coordinator of energy metabolism and survival in HCC cells. Article Title: Lactate-associated H3K18 lactylation and SYT8 in the temozolomide response of glioblastoma cells. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Purification:Article Title: LAMTOR2 Reprograms Energy Metabolism to Sustain Hepatocellular Carcinoma Cell Survival Article Snippet: Hepatocellular carcinoma (HCC) is characterized by profound metabolic reprogramming to support rapid proliferation and survival under nutrient stress.. However, the central regulatory hubs that coordinate these diverse metabolic programs remain poorly understood.. Here, we identify LAMTOR2 as a critical coordinator of energy metabolism and survival in HCC cells. Gel Extraction:Article Title: LAMTOR2 Reprograms Energy Metabolism to Sustain Hepatocellular Carcinoma Cell Survival Article Snippet: Hepatocellular carcinoma (HCC) is characterized by profound metabolic reprogramming to support rapid proliferation and survival under nutrient stress.. However, the central regulatory hubs that coordinate these diverse metabolic programs remain poorly understood.. Here, we identify LAMTOR2 as a critical coordinator of energy metabolism and survival in HCC cells. Over Expression:Article Title: tRNA dosage regulates lineage dependency and resistance in prostate cancer. Article Snippet: .. For overexpression of tRNAArg(UCU) isodecoders, three tandem repeats of tRNA1 Arg(UCU), tRNA2 Arg(UCU), tRNA3 Arg(UCU), tRNA4 Arg(UCU) and tRNA5 Arg(UCU) and their flanking sequences around the genomic loci (±200 bp) (see Supplementary Table 9 for details) were cloned into Article Title: CircACVR2A(2-4) regulates stem cell pluripotency by uncoupling CDK-activating kinase functions. Article Snippet: .. Short hairpin RNA and circRNA overexpression constructs All human RNA-targeting shRNAs were cloned into Clone Assay:Article Title: tRNA dosage regulates lineage dependency and resistance in prostate cancer. Article Snippet: .. For overexpression of tRNAArg(UCU) isodecoders, three tandem repeats of tRNA1 Arg(UCU), tRNA2 Arg(UCU), tRNA3 Arg(UCU), tRNA4 Arg(UCU) and tRNA5 Arg(UCU) and their flanking sequences around the genomic loci (±200 bp) (see Supplementary Table 9 for details) were cloned into Article Title: Lactate-associated H3K18 lactylation and SYT8 in the temozolomide response of glioblastoma cells. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Article Title: CircACVR2A(2-4) regulates stem cell pluripotency by uncoupling CDK-activating kinase functions. Article Snippet: .. Short hairpin RNA and circRNA overexpression constructs All human RNA-targeting shRNAs were cloned into Knockdown:Article Title: DNA replication stress redistributes the histone methyltransferase G9a and activates lineage-specific gene expression Article Snippet: .. HRI knockdown was performed using shRNA constructs (TRCN0000010229 and TRCN0000010231 from the Broad Genome Perturbation Platform) expressed from a Article Title: Fanconi anemia pathway disruption drives lomustine sensitivity in glioma. Article Snippet: 209 Apoptosis Analysis 210 Apoptosis signal under CCNU and PIP-199 treatment in different cell lines was assessed with the BD 211 Annexin V/PI detection Kit (BD, Franklin Lakes, NJ, USA) according to the manufacturer’s protocol as 212 previously described.21 Further information can be found in the Supplementary information. .. 213 Primary glioblastoma cultures 214 Primary cultures were generated and treated as previously described and outlined in the 215 Supplementary Methods.19,21 216 shRNA knockdown human cell lines (lentivirus) 217 We introduced shRNA sequences (Supplementary Table ST4) into Construct:Article Title: DNA replication stress redistributes the histone methyltransferase G9a and activates lineage-specific gene expression Article Snippet: .. HRI knockdown was performed using shRNA constructs (TRCN0000010229 and TRCN0000010231 from the Broad Genome Perturbation Platform) expressed from a Article Title: A natural compound derivative targeting HADHA disrupts mitochondrial homeostasis and suppresses renal cell carcinoma. Article Snippet: Ara Jo, Sanjeevram Dhandapani, Ziqin Wang, Hyeryeon Jung, Hongjun Jang, Morgan A. Bailey, Andrew Tran, Brandon Tsai, Jinjae Park, Hyejin Lee, Hye-Jin Park, Yoojin Lim, Dolce Feenaghty, Victor M. Moncada, Damu Wu, Keaton Chien, Nancy Chen, Yunsol Jo, Jiazheng Miao, Zhujun Yao, Jihoon Lee, Karthigayan Shanmugasundaram, Gang Greg Wang, Andrew Armstrong, Daniel George, David Howell, Jiaoti Huang, Michael C. Fitzgerald, Jiyong Hong, Jung Wook Park Article Title: CircACVR2A(2-4) regulates stem cell pluripotency by uncoupling CDK-activating kinase functions. Article Snippet: .. Short hairpin RNA and circRNA overexpression constructs All human RNA-targeting shRNAs were cloned into Generated:Article Title: Fanconi anemia pathway disruption drives lomustine sensitivity in glioma. Article Snippet: 209 Apoptosis Analysis 210 Apoptosis signal under CCNU and PIP-199 treatment in different cell lines was assessed with the BD 211 Annexin V/PI detection Kit (BD, Franklin Lakes, NJ, USA) according to the manufacturer’s protocol as 212 previously described.21 Further information can be found in the Supplementary information. .. 213 Primary glioblastoma cultures 214 Primary cultures were generated and treated as previously described and outlined in the 215 Supplementary Methods.19,21 216 shRNA knockdown human cell lines (lentivirus) 217 We introduced shRNA sequences (Supplementary Table ST4) into |
