Virus:Article Title: Multicistronic miRNA delivery for cardiac reprogramming
Article Snippet: .. Generation of self-complementary adeno-associated virus (scAAV): scAAV was generated by sub-cloning the CMV promoter, miR combo construct 1, and the BGH polyadenylation sequence from the pcDNA3.1 plasmid into the MCS of the tr1TR2-Basic (+) plasmid (Addgene). ..
Generated:Article Title: Multicistronic miRNA delivery for cardiac reprogramming
Article Snippet: .. Generation of self-complementary adeno-associated virus (scAAV): scAAV was generated by sub-cloning the CMV promoter, miR combo construct 1, and the BGH polyadenylation sequence from the pcDNA3.1 plasmid into the MCS of the tr1TR2-Basic (+) plasmid (Addgene). ..
Construct:Article Title: Multicistronic miRNA delivery for cardiac reprogramming
Article Snippet: .. Generation of self-complementary adeno-associated virus (scAAV): scAAV was generated by sub-cloning the CMV promoter, miR combo construct 1, and the BGH polyadenylation sequence from the pcDNA3.1 plasmid into the MCS of the tr1TR2-Basic (+) plasmid (Addgene). ..
Article Title: Overproduction of ascorbic acid impairs pollen fertility in tomato.
Article Snippet: CRISPR/Cas9 mutagenesis was performed on cotyledons as described in Bollier et al. (2018), except for the replacement of the level 1 construct pICH47742:2x35S-5′UTR-hCas9(STOP)-NOST by pICH47742:2x35S::Cas9n, which was subcloned from pDicAID_nCas9PmCDA_NptII_Della (Kondo, Addgene #91694). .. The level 1 construct was assembled with level 2 plCSL4723 (Addgene plasmid #86172). ..
Article Title: Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Article Title: TESOGENASE, An Engineered Nuclease Editor for Enhanced Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..
Article Title: Ion channel inhibition by targeted recruitment of NEDD4-2 with divalent nanobodies
Article Snippet: We used Gibson assembly to clone the mVenus fluorescence protein into the C-terminal position of the human leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5; a gift from Toshiro Sato; Addgene plasmid # 129094) . .. The Succinate Receptor 1 plasmid construct (SUCCR1) was a gift from Bryan Roth (Addgene plasmid # 66507) . ..
Article Title: Polyglutamylation of tubulin's C-terminal tail controls pausing and motility of kinesin-3 family member KIF1A
Article Snippet: .. We thank Gary Banker & Marvin Bentley for the gifted pBa.Kif1a 1-396.GFP (Addgene plasmid # 45058), William Hancock for the use of his kinesin-1 construct, and Adam Hendricks for support. ..
Sequencing:Article Title: Multicistronic miRNA delivery for cardiac reprogramming
Article Snippet: .. Generation of self-complementary adeno-associated virus (scAAV): scAAV was generated by sub-cloning the CMV promoter, miR combo construct 1, and the BGH polyadenylation sequence from the pcDNA3.1 plasmid into the MCS of the tr1TR2-Basic (+) plasmid (Addgene). ..
Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..
Plasmid Preparation:Article Title: Multicistronic miRNA delivery for cardiac reprogramming
Article Snippet: .. Generation of self-complementary adeno-associated virus (scAAV): scAAV was generated by sub-cloning the CMV promoter, miR combo construct 1, and the BGH polyadenylation sequence from the pcDNA3.1 plasmid into the MCS of the tr1TR2-Basic (+) plasmid (Addgene). ..
Article Title: Overproduction of ascorbic acid impairs pollen fertility in tomato.
Article Snippet: CRISPR/Cas9 mutagenesis was performed on cotyledons as described in Bollier et al. (2018), except for the replacement of the level 1 construct pICH47742:2x35S-5′UTR-hCas9(STOP)-NOST by pICH47742:2x35S::Cas9n, which was subcloned from pDicAID_nCas9PmCDA_NptII_Della (Kondo, Addgene #91694). .. The level 1 construct was assembled with level 2 plCSL4723 (Addgene plasmid #86172). ..
Article Title: Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Article Title: TESOGENASE, An Engineered Nuclease Editor for Enhanced Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..
Article Title: Ion channel inhibition by targeted recruitment of NEDD4-2 with divalent nanobodies
Article Snippet: We used Gibson assembly to clone the mVenus fluorescence protein into the C-terminal position of the human leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5; a gift from Toshiro Sato; Addgene plasmid # 129094) . .. The Succinate Receptor 1 plasmid construct (SUCCR1) was a gift from Bryan Roth (Addgene plasmid # 66507) . ..
Article Title: Polyglutamylation of tubulin's C-terminal tail controls pausing and motility of kinesin-3 family member KIF1A
Article Snippet: .. We thank Gary Banker & Marvin Bentley for the gifted pBa.Kif1a 1-396.GFP (Addgene plasmid # 45058), William Hancock for the use of his kinesin-1 construct, and Adam Hendricks for support. ..
Binding Assay:Article Title: Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Article Title: TESOGENASE, An Engineered Nuclease Editor for Enhanced Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Synthesized:Article Title: Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Article Title: TESOGENASE, An Engineered Nuclease Editor for Enhanced Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Modification:Article Title: Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Article Title: TESOGENASE, An Engineered Nuclease Editor for Enhanced Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Cloning:Article Title: Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Article Title: TESOGENASE, An Engineered Nuclease Editor for Enhanced Targeted Genome Integration
Article Snippet: .. For all-in-one (AIO) plasmid construct design for various enGagers (Table 1), ssDNA binding protein and peptide sequences were synthesized and assembled into an AIO plasmid modified from Addgene plasmid #42230 by golden gate cloning. ..
Transfection:
Luciferase:
Marker:Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..
Derivative Assay:Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..
Expressing:Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..
Amplification:Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..
Activation Assay:Article Title: Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation
Article Snippet: .. Plasmid Construct Source 1. pRS313 Yeast centromere vector with a HIS3 marker and an MCS derived from pBLUESCRIPT Addgene Vectors 2. pAFS125 GFP-TUB1 expression cassette for integration at the chromosomal URA3 locus (90) 3. pAKD06+CENIV Yeast centromere vector with a URA3 marker and another CEN between GAL1 promoter and lacZα sequence Prof. Santanu Kumar Ghosh, IIT-Bombay 4. pAG32 Episomal vector with HPH marker Prof. Santanu Kumar Ghosh, IIT-Bombay 5. pUG73 Episomal vector with LEU2 marker Prof. Santanu Kumar Ghosh, IIT-Bombay 6. pAW8-mCherry Used for amplification of mCherry for gene tagging Prof. Santanu Kumar Ghosh, IIT-Bombay 7. pGAD-C1 Yeast two-hybrid prey vector for fusing a gene to the GAL4 activation domain Prof. Santanu Kumar Ghosh, IIT-Bombay 8. pGBD-C1 Yeast two-hybrid bait vector for fusing a gene to the GAL4 DNA-binding domain Prof. Santanu Kumar Ghosh, IIT-Bombay 9. pFA6A- HIS3MX6pGAL1-VC155 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 10. pFA6A- KANMX6pGAL1-VN173 Used for N-terminal tagging of proteins for bimolecular fluorescence complementation (BiFC) assay Prof. Santanu Kumar Ghosh, IIT-Bombay 11. pGADC1-ATG11 ATG11 fused with the GAL4 activation domain This study 12. pGBDC1-SPC72 SPC72 fused with the GAL4 DNA-binding domain This study 13. pGBDC1-ATG11 ATG11 fused with the GAL4 DNA-binding domain This study 14. pGADC1-SPC72 SPC72 fused with the GAL4 activation domain This study 15. pGADC1-CNM67 CNM67 fused with the GAL4 activation domain This study 16. pYM19 Used for tagging Clb4 with 9x-Myc (91) 17. pYM16 Used for tagging Pds1 with 6x-HA (91) 18. pTSK561 Used for tagging Atg11 with Halo tag Addgene ..
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