Review



plasmid vector constructs 449 zebrafish liver zfl cells  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    ATCC plasmid vector constructs 449 zebrafish liver zfl cells
    Plasmid Vector Constructs 449 Zebrafish Liver Zfl Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+constructs/ZFL/pm42219039-231-0-9
    Average 95 stars, based on 181 article reviews
    plasmid vector constructs 449 zebrafish liver zfl cells - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Amuc_1100 alleviates HFD-induced hepatic lipid accumulation via gut microbiota in zebrafish: insights from the role of intestinal 14-3-3β/α-A.
    Article Snippet: .. The ZFL (ATCC ® CRL-2643TM), ZF4 (ATCC ® CRL2050TM) and HEK293T (ATCC ® CRL-3216TM) cell lines were purchased from American Type Culture Collection (Manassas, VA, USA), and cultured according to an established protocol [43, 44]. .. All media were obtained from Corning Inc. Penicillin-Streptomycin solution and bovine insulin were purchased from Sigma (St. Louis, MO, USA).

    Article Title: Tiered Genotoxicity Testing of Enriched River Water Samples Using Zebrafish In Vitro and In Vivo Models: A Joint Danube Survey 4 Case Study
    Article Snippet: .. ZFL cells, derived from normal liver of adult zebrafish, were obtained from the American Type Culture Collection (ATCC number: CRL-2634) and were cultured at 28 ◦C in a humidified air atmosphere in a medium composed of 50 % Leibovitz L-15, 35 % DMEM, and 15 % Ham F12, supplemented with 15 mM HEPES, 0.15 g/L NaHCO3, 0.01 mg/mL bovine insulin, 50 ng/mL mouse EGF, 0.1 % penicillin/streptomycin, and 5 % heath inactivated FBS. ..

    Article Title: Developmental hepatotoxicity induced by flusilazole in zebrafish: Mechanistic insights into mitochondrial dysfunction, oxidative stress, ferroptosis, and regenerative impairment.
    Article Snippet: Flusilazole is a triazole-based fungicide that persists in various environments because of its high stability and solubility, raising concerns about its developmental and ecological impacts.. Although numerous studies have reported flusilazole-induced toxicity, the specific effects and mechanisms of flusilazole-induced hepatotoxicity during development remain unclear.. In this study, we examined the in vivo and in vitro toxicities in Danio rerio (zebrafish) and zebrafish-derived liver (ZFL) cells.

    Recombinant:

    Article Title: CYP1A1-targeted monoclonal antibody with reactivity across vertebrate taxa
    Article Snippet: Animal Cell Lines and Tissues Origin Source ZFL Zebrafish liver cells ATCC CRL-2643 PLHC-1 Top minnow hepatocellular ATCC CRL-2406 carcinoma Hepa1c1c7 Mouse liver hepatoma ATCC CRL-2026 XLK-WG African clawed frog kidney ATCC CRL-2527 epithelial HEK293 Human embryonic kidney ATCC CRL-3467 Rat liver S9 Arochlor-1254-induced P450s MOLTOX 11101.5 fraction Rat liver S9 Non-induced livers MOLTOX 11102.2 fraction Recombinant Over-expressed in HEK293 cell OriGene LC400170 human lysates CYP1A Mouse livers PCB-126-induced CYP1A (and DeLong and Rice, 1997 for IHC controls) J. Toxicol. .. Animal Cell Lines and Tissues Origin Source ZFL Zebrafish liver cells ATCC CRL-2643 PLHC-1 Top minnow hepatocellular ATCC CRL-2406 carcinoma Hepa1c1c7 Mouse liver hepatoma ATCC CRL-2026 XLK-WG African clawed frog kidney ATCC CRL-2527 epithelial HEK293 Human embryonic kidney ATCC CRL-3467 Rat liver S9 Arochlor-1254-induced P450s MOLTOX 11101.5 fraction Rat liver S9 Non-induced livers MOLTOX 11102.2 fraction Recombinant Over-expressed in HEK293 cell OriGene LC400170 human lysates CYP1A Mouse livers PCB-126-induced CYP1A (and DeLong and Rice, 1997 for IHC controls) J. Toxicol. ..

    Immunohistochemistry:

    Article Title: CYP1A1-targeted monoclonal antibody with reactivity across vertebrate taxa
    Article Snippet: Animal Cell Lines and Tissues Origin Source ZFL Zebrafish liver cells ATCC CRL-2643 PLHC-1 Top minnow hepatocellular ATCC CRL-2406 carcinoma Hepa1c1c7 Mouse liver hepatoma ATCC CRL-2026 XLK-WG African clawed frog kidney ATCC CRL-2527 epithelial HEK293 Human embryonic kidney ATCC CRL-3467 Rat liver S9 Arochlor-1254-induced P450s MOLTOX 11101.5 fraction Rat liver S9 Non-induced livers MOLTOX 11102.2 fraction Recombinant Over-expressed in HEK293 cell OriGene LC400170 human lysates CYP1A Mouse livers PCB-126-induced CYP1A (and DeLong and Rice, 1997 for IHC controls) J. Toxicol. .. Animal Cell Lines and Tissues Origin Source ZFL Zebrafish liver cells ATCC CRL-2643 PLHC-1 Top minnow hepatocellular ATCC CRL-2406 carcinoma Hepa1c1c7 Mouse liver hepatoma ATCC CRL-2026 XLK-WG African clawed frog kidney ATCC CRL-2527 epithelial HEK293 Human embryonic kidney ATCC CRL-3467 Rat liver S9 Arochlor-1254-induced P450s MOLTOX 11101.5 fraction Rat liver S9 Non-induced livers MOLTOX 11102.2 fraction Recombinant Over-expressed in HEK293 cell OriGene LC400170 human lysates CYP1A Mouse livers PCB-126-induced CYP1A (and DeLong and Rice, 1997 for IHC controls) J. Toxicol. ..

    Derivative Assay:

    Article Title: Tiered Genotoxicity Testing of Enriched River Water Samples Using Zebrafish In Vitro and In Vivo Models: A Joint Danube Survey 4 Case Study
    Article Snippet: .. ZFL cells, derived from normal liver of adult zebrafish, were obtained from the American Type Culture Collection (ATCC number: CRL-2634) and were cultured at 28 ◦C in a humidified air atmosphere in a medium composed of 50 % Leibovitz L-15, 35 % DMEM, and 15 % Ham F12, supplemented with 15 mM HEPES, 0.15 g/L NaHCO3, 0.01 mg/mL bovine insulin, 50 ng/mL mouse EGF, 0.1 % penicillin/streptomycin, and 5 % heath inactivated FBS. ..

    Isolation:

    Article Title: Developmental hepatotoxicity induced by flusilazole in zebrafish: Mechanistic insights into mitochondrial dysfunction, oxidative stress, ferroptosis, and regenerative impairment.
    Article Snippet: Flusilazole is a triazole-based fungicide that persists in various environments because of its high stability and solubility, raising concerns about its developmental and ecological impacts.. Although numerous studies have reported flusilazole-induced toxicity, the specific effects and mechanisms of flusilazole-induced hepatotoxicity during development remain unclear.. In this study, we examined the in vivo and in vitro toxicities in Danio rerio (zebrafish) and zebrafish-derived liver (ZFL) cells.

    other:

    Article Title: Bacillus velezensis -derived muropeptide promotes growth of zebrafish via NOD2-mediated induction of IGF1 signaling
    Article Snippet: The culture method of zebrafish liver cells (ZFL, ATCC CRL-2643) was as described previously [ ].

    Article Title: Developing a pan cancer therapy based on DISE inducing short RNAs
    Article Snippet: HAP1 (human chronic myeloid leukemia, Horizon Genomics) and PC3 (human prostate cancer, 339 ATCC/LGC Promochem, Wesel, Germany) were cultured in Iscove's Modified Dulbecco's Medium (IMDM) 340 (Gibco Life Technologies cat#12440053), 10 % FBS, 1 % L-Glut.

    Plasmid Preparation:

    Article Title: GRP75 Chaperone-driven Mitochondria-ER Membrane Coupling Promotes Ca 2+ -Dependent ROS Accumulation and Ferroptosis During Hypoxic Stress.
    Article Snippet: .. Plasmid vector constructs 449 Zebrafish liver (ZFL) cells (CRL-2643, ATCC) were maintained in Ham's F-12 450 medium containing 10% heat-inactivated FBS, with incubation conditions set at 28 °C 451 under 5% CO2 atmosphere. ..

    Construct:

    Article Title: GRP75 Chaperone-driven Mitochondria-ER Membrane Coupling Promotes Ca 2+ -Dependent ROS Accumulation and Ferroptosis During Hypoxic Stress.
    Article Snippet: .. Plasmid vector constructs 449 Zebrafish liver (ZFL) cells (CRL-2643, ATCC) were maintained in Ham's F-12 450 medium containing 10% heat-inactivated FBS, with incubation conditions set at 28 °C 451 under 5% CO2 atmosphere. ..

    Incubation:

    Article Title: GRP75 Chaperone-driven Mitochondria-ER Membrane Coupling Promotes Ca 2+ -Dependent ROS Accumulation and Ferroptosis During Hypoxic Stress.
    Article Snippet: .. Plasmid vector constructs 449 Zebrafish liver (ZFL) cells (CRL-2643, ATCC) were maintained in Ham's F-12 450 medium containing 10% heat-inactivated FBS, with incubation conditions set at 28 °C 451 under 5% CO2 atmosphere. ..



    Similar Products

    96
    Addgene inc crispr guide constructs targeting tet1
    Crispr Guide Constructs Targeting Tet1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+constructs/lentiCRISPR+v2-Blast+(Plasmid+%2383480)/pm42869907-371-0-18
    Average 96 stars, based on 1 article reviews
    crispr guide constructs targeting tet1 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    94
    Addgene inc loxp dsred loxp egfp cre reporter construct
    Loxp Dsred Loxp Egfp Cre Reporter Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+constructs/CMV-loxp-DsRed-loxp-eGFP+(Plasmid+%23141148)/pm42834039-604-6-10
    Average 94 stars, based on 1 article reviews
    loxp dsred loxp egfp cre reporter construct - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    New England Biolabs plasmid constructs
    Plasmid Constructs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+constructs/PsiI-v2/pm42834082-376-1-7
    Average 94 stars, based on 1 article reviews
    plasmid constructs - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    96
    Addgene inc viral construct
    Viral Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+constructs/pAAV-hSyn-DIO-hM3D(Gq)-mCherry+(Plasmid+%2344361)/pm42830177-55-3-6
    Average 96 stars, based on 1 article reviews
    viral construct - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    99
    Plasmidsaurus plasmid construction
    Plasmid Construction, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+constructs/Plasmid/10__1177_slash_1540658x261490394-133-1-19
    Average 99 stars, based on 1 article reviews
    plasmid construction - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    94
    Addgene inc lentiviral plko 1 shrna construct shgfp
    Lentiviral Plko 1 Shrna Construct Shgfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+constructs/pLKO%2E1+puro+GFP+siRNA+(Plasmid+%2312273)/10__1158_slash_0008___5472__can___26___1063-43-1-6
    Average 94 stars, based on 1 article reviews
    lentiviral plko 1 shrna construct shgfp - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Addgene inc megfp c1 construct
    Megfp C1 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+constructs/mEGFP-C1+(Plasmid+%2354759)/10__1038_slash_s41467___026___78058___y-607-22-24
    Average 94 stars, based on 1 article reviews
    megfp c1 construct - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    99
    Plasmidsaurus plasmid constructs
    Plasmid Constructs, supplied by Plasmidsaurus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+constructs/Plasmid/pm42789393-1076-1-14
    Average 99 stars, based on 1 article reviews
    plasmid constructs - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    93
    Addgene inc lentiviral constructs
    (A) Genome schematics of DVG-B (left) and DVG-ΔN (right). (B) A549-ACE2 cells were infected with WT, DVG-B or DVG-ΔN at MOI 1 and cells were collected 24-hours post-infection for RT-qPCR. Expression of host genes and viral genes/eGFP were calculated relative to GAPDH. **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-way ANOVA with Tukey’s multiple comparisons test. N = 3, mean±SD. (C) A549-ACE2 cells were infected with WT (MOI 0.1), DVG-B or DVG-ΔN (MOI 1) and cells were collected 24-hours post-inoculation for confocal microscopy. Representative maximum intensity projections of merged dsRNA (red) staining. Nuclei were Hoechst stained (blue). GFP (green) indicates DVG-B and DVG-ΔN, respectively. Scale bar indicates 20μm. N = 4 fields for WT, DVG-B and DVG-ΔN, respectively. (D) A549-ACE2 cells were transduced with <t>lentiviral</t> vectors encoding SARS-CoV-2 orf9b (ORF9b) or an empty lentiviral vector control (EV). 48 hours post-transduction, cells were infected with DVG-ΔN (MOI 1). 24 hpi cells were collected for RT-qPCR. Expression of host and viral genes were calculated relative to GAPDH. *p < 0.05, by two-way ANOVA with Bonferroni’s multiple comparisons test. N = 3, mean±SD.
    Lentiviral Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+constructs/pCph8+(Plasmid+%2322869)/pmc13626462-492-0-5
    Average 93 stars, based on 1 article reviews
    lentiviral constructs - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    (A) Genome schematics of DVG-B (left) and DVG-ΔN (right). (B) A549-ACE2 cells were infected with WT, DVG-B or DVG-ΔN at MOI 1 and cells were collected 24-hours post-infection for RT-qPCR. Expression of host genes and viral genes/eGFP were calculated relative to GAPDH. **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-way ANOVA with Tukey’s multiple comparisons test. N = 3, mean±SD. (C) A549-ACE2 cells were infected with WT (MOI 0.1), DVG-B or DVG-ΔN (MOI 1) and cells were collected 24-hours post-inoculation for confocal microscopy. Representative maximum intensity projections of merged dsRNA (red) staining. Nuclei were Hoechst stained (blue). GFP (green) indicates DVG-B and DVG-ΔN, respectively. Scale bar indicates 20μm. N = 4 fields for WT, DVG-B and DVG-ΔN, respectively. (D) A549-ACE2 cells were transduced with lentiviral vectors encoding SARS-CoV-2 orf9b (ORF9b) or an empty lentiviral vector control (EV). 48 hours post-transduction, cells were infected with DVG-ΔN (MOI 1). 24 hpi cells were collected for RT-qPCR. Expression of host and viral genes were calculated relative to GAPDH. *p < 0.05, by two-way ANOVA with Bonferroni’s multiple comparisons test. N = 3, mean±SD.

    Journal: PLOS Pathogens

    Article Title: SARS-CoV-2 defective viral genomes from distinct genomic regions drive divergent interferon responses

    doi: 10.1371/journal.ppat.1014099

    Figure Lengend Snippet: (A) Genome schematics of DVG-B (left) and DVG-ΔN (right). (B) A549-ACE2 cells were infected with WT, DVG-B or DVG-ΔN at MOI 1 and cells were collected 24-hours post-infection for RT-qPCR. Expression of host genes and viral genes/eGFP were calculated relative to GAPDH. **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-way ANOVA with Tukey’s multiple comparisons test. N = 3, mean±SD. (C) A549-ACE2 cells were infected with WT (MOI 0.1), DVG-B or DVG-ΔN (MOI 1) and cells were collected 24-hours post-inoculation for confocal microscopy. Representative maximum intensity projections of merged dsRNA (red) staining. Nuclei were Hoechst stained (blue). GFP (green) indicates DVG-B and DVG-ΔN, respectively. Scale bar indicates 20μm. N = 4 fields for WT, DVG-B and DVG-ΔN, respectively. (D) A549-ACE2 cells were transduced with lentiviral vectors encoding SARS-CoV-2 orf9b (ORF9b) or an empty lentiviral vector control (EV). 48 hours post-transduction, cells were infected with DVG-ΔN (MOI 1). 24 hpi cells were collected for RT-qPCR. Expression of host and viral genes were calculated relative to GAPDH. *p < 0.05, by two-way ANOVA with Bonferroni’s multiple comparisons test. N = 3, mean±SD.

    Article Snippet: Lentiviral constructs were obtained from addgene ( https://doi.org/10.1038/s41586-020-2286-9 ).

    Techniques: Infection, Quantitative RT-PCR, Expressing, Confocal, Microscopy, Staining, Transduction, Plasmid Preparation, Control