Enzyme-linked Immunosorbent Assay:Article Title: Metformin increases glycolysis and the stress-induced cytokine GDF15 but not FGF21 in humans
Article Snippet: .. Media stored at -80 °C was thawed on ice, and GDF15 and FGF21 protein levels were measured using the Human GDF-15 Quantikine ELISA Kit (R&D Systems, MN, US) and Human FGF-21 Quantikine ELISA Kit (R&D Systems, MN, US) according to the manufacturer’s protocol. .. The optical density was measured on a Spectramax Plus 384 Microplate Reader (Molecular Devices, CA, USA) at wavelengths of 450 nm and 570 nm.
Article Title: Charting the circulating proteome in ME/CFS using cross-system profiling to uncover mechanistic insights.
Article Snippet: .. Serum samples were stored at − 80 ◦ C. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commerical assays Custom Luminex Human Discovery assay R&D Systems Cat# LXSAHM; RRID:AB_2924693 Quantikine Ready-To-Use ELISA kit (FGF-21) R&D Systems Cat# DF2100; RRID:AB_2783729 Quantikine Ready-To-Use ELISA kit (GDF-15) R&D Systems Cat# DGD150; RRID:AB_2877710 Quantikine Ready-To-Use ELISA kit (C-peptide) R&D Systems Cat# DICP00 SomaScan Discovery, 7 K SomaLogic Inc v. 4.1 Biological samples Human serum samples ME/CFS Biobank, Haukeland University Hospital, Norway RituxME: NCT02229942 CycloME: NCT02444091 Deposited data Proteomics dataset Deposited in the PRIDE 90 repository (PRIDE: PAD000026) N/A Metabolomics data Hoel et al. 17 ; DOI: https://doi.org/ 10.1172/jci.insight.149217 N/A Software and algorithms GraphPad Prism 9 GraphPad Software N/A R (4.5.1) https://www.r-project.org N/A Rstudio (2025.09.1 + 401) Posit Software N/A R package: Vegan (2.7.0) DOI: https://doi.org/10.32614/CRAN.package.vegan N/A R package: limma (3.64.3) DOI: https://doi.org/10.18129/B9.bioc.limma N/A R package: variancePartition (1.38.1) DOI: https://doi.org/10.18129/B9.bioc.variancePartition N/A R package: ppcor (1.1) DOI: https://doi.org/10.32614/CRAN.package.ppcor N/A R package: clusterProfiler (4.16.0) DOI: https://doi.org/10.18129/B9.bioc.clusterProfiler N/A R package: ComplexHeatmap (2.24.1) DOI: https://doi.org/10.18129/B9.bioc.ComplexHeatmap N/A R package: ggplot2 (4.0.0) DOI: https://doi.org/10.32614/CRAN.package.ggplot2 N/A R package: tidygraph (1.3.1) DOI: https://doi.org/10.32614/CRAN.package.tidygraph N/A R package: igraph (2.2.0) DOI: https://doi.org/10.32614/CRAN.package.igraph N/A R package: ggpubr (0.6.2) DOI: https://doi.org/10.32614/CRAN.package.ggpubr N/A Cellinker DOI: https://doi.org/10.1093/bioinformatics/btab036 N/A BioRender BioRender N/A Microsoft Office Excel Microsoft N/A Other Human protein atlas (HPA) https://www.proteinatlas.org/ N/A Cell Reports Medicine 7, 102647, March 17, 2026 e1 OPEN ACCESS In total, 54 ME/CFS and 29 HC participants were analyzed on the aptamer-platform. ..
Luminex:Article Title: Charting the circulating proteome in ME/CFS using cross-system profiling to uncover mechanistic insights.
Article Snippet: .. Serum samples were stored at − 80 ◦ C. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commerical assays Custom Luminex Human Discovery assay R&D Systems Cat# LXSAHM; RRID:AB_2924693 Quantikine Ready-To-Use ELISA kit (FGF-21) R&D Systems Cat# DF2100; RRID:AB_2783729 Quantikine Ready-To-Use ELISA kit (GDF-15) R&D Systems Cat# DGD150; RRID:AB_2877710 Quantikine Ready-To-Use ELISA kit (C-peptide) R&D Systems Cat# DICP00 SomaScan Discovery, 7 K SomaLogic Inc v. 4.1 Biological samples Human serum samples ME/CFS Biobank, Haukeland University Hospital, Norway RituxME: NCT02229942 CycloME: NCT02444091 Deposited data Proteomics dataset Deposited in the PRIDE 90 repository (PRIDE: PAD000026) N/A Metabolomics data Hoel et al. 17 ; DOI: https://doi.org/ 10.1172/jci.insight.149217 N/A Software and algorithms GraphPad Prism 9 GraphPad Software N/A R (4.5.1) https://www.r-project.org N/A Rstudio (2025.09.1 + 401) Posit Software N/A R package: Vegan (2.7.0) DOI: https://doi.org/10.32614/CRAN.package.vegan N/A R package: limma (3.64.3) DOI: https://doi.org/10.18129/B9.bioc.limma N/A R package: variancePartition (1.38.1) DOI: https://doi.org/10.18129/B9.bioc.variancePartition N/A R package: ppcor (1.1) DOI: https://doi.org/10.32614/CRAN.package.ppcor N/A R package: clusterProfiler (4.16.0) DOI: https://doi.org/10.18129/B9.bioc.clusterProfiler N/A R package: ComplexHeatmap (2.24.1) DOI: https://doi.org/10.18129/B9.bioc.ComplexHeatmap N/A R package: ggplot2 (4.0.0) DOI: https://doi.org/10.32614/CRAN.package.ggplot2 N/A R package: tidygraph (1.3.1) DOI: https://doi.org/10.32614/CRAN.package.tidygraph N/A R package: igraph (2.2.0) DOI: https://doi.org/10.32614/CRAN.package.igraph N/A R package: ggpubr (0.6.2) DOI: https://doi.org/10.32614/CRAN.package.ggpubr N/A Cellinker DOI: https://doi.org/10.1093/bioinformatics/btab036 N/A BioRender BioRender N/A Microsoft Office Excel Microsoft N/A Other Human protein atlas (HPA) https://www.proteinatlas.org/ N/A Cell Reports Medicine 7, 102647, March 17, 2026 e1 OPEN ACCESS In total, 54 ME/CFS and 29 HC participants were analyzed on the aptamer-platform. ..
Software:Article Title: Charting the circulating proteome in ME/CFS using cross-system profiling to uncover mechanistic insights.
Article Snippet: .. Serum samples were stored at − 80 ◦ C. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commerical assays Custom Luminex Human Discovery assay R&D Systems Cat# LXSAHM; RRID:AB_2924693 Quantikine Ready-To-Use ELISA kit (FGF-21) R&D Systems Cat# DF2100; RRID:AB_2783729 Quantikine Ready-To-Use ELISA kit (GDF-15) R&D Systems Cat# DGD150; RRID:AB_2877710 Quantikine Ready-To-Use ELISA kit (C-peptide) R&D Systems Cat# DICP00 SomaScan Discovery, 7 K SomaLogic Inc v. 4.1 Biological samples Human serum samples ME/CFS Biobank, Haukeland University Hospital, Norway RituxME: NCT02229942 CycloME: NCT02444091 Deposited data Proteomics dataset Deposited in the PRIDE 90 repository (PRIDE: PAD000026) N/A Metabolomics data Hoel et al. 17 ; DOI: https://doi.org/ 10.1172/jci.insight.149217 N/A Software and algorithms GraphPad Prism 9 GraphPad Software N/A R (4.5.1) https://www.r-project.org N/A Rstudio (2025.09.1 + 401) Posit Software N/A R package: Vegan (2.7.0) DOI: https://doi.org/10.32614/CRAN.package.vegan N/A R package: limma (3.64.3) DOI: https://doi.org/10.18129/B9.bioc.limma N/A R package: variancePartition (1.38.1) DOI: https://doi.org/10.18129/B9.bioc.variancePartition N/A R package: ppcor (1.1) DOI: https://doi.org/10.32614/CRAN.package.ppcor N/A R package: clusterProfiler (4.16.0) DOI: https://doi.org/10.18129/B9.bioc.clusterProfiler N/A R package: ComplexHeatmap (2.24.1) DOI: https://doi.org/10.18129/B9.bioc.ComplexHeatmap N/A R package: ggplot2 (4.0.0) DOI: https://doi.org/10.32614/CRAN.package.ggplot2 N/A R package: tidygraph (1.3.1) DOI: https://doi.org/10.32614/CRAN.package.tidygraph N/A R package: igraph (2.2.0) DOI: https://doi.org/10.32614/CRAN.package.igraph N/A R package: ggpubr (0.6.2) DOI: https://doi.org/10.32614/CRAN.package.ggpubr N/A Cellinker DOI: https://doi.org/10.1093/bioinformatics/btab036 N/A BioRender BioRender N/A Microsoft Office Excel Microsoft N/A Other Human protein atlas (HPA) https://www.proteinatlas.org/ N/A Cell Reports Medicine 7, 102647, March 17, 2026 e1 OPEN ACCESS In total, 54 ME/CFS and 29 HC participants were analyzed on the aptamer-platform. ..
other:Article Title: Association of serum fibroblast growth factor 21 levels with coronary artery calcification and coronary intervention in patients with chronic total occlusion
Article Snippet: Serum FGF21 levels were measured by ELISA (DF2100; R&D Systems, Minneapolis, Minnesota, USA), according to the manufacturer’s instructions.
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![Fig. 3. Effect of FGH21 addition on GH signaling. A, B) Immunoblotting of JAK2, p-JAK2, STAT5b, and HSP90. Fao cells were cultured in the Full or Zero medium with 0–10 [5] pM of recombinant <t>FGF21</t> for 24 h. Cells were then treated with 100 nM GH or vehicle for 10 min. C) Igf1 mRNA levels were quantified using real-time qPCR. Values were normalized against the Actb mRNA level. Fao cells were cultured in the full medium with or without 100 nM GH and 0–105 pM of FGF21 for 24 h. Total RNA was isolated. Bar: means ± SEM (n = 3), *p < 0.05. (D) The expression of α-klotho (Kl), β-klotho (Klb), and FGF Receptor (Fgfr) 1–4 in Fao cells, rat primary hepatocytes, and rat tissue samples were analyzed using reverse transcription-PCR. (E) Immnoblotting of Erk and p-Erk. Fao cells were cultured in the full medium for 24 h. The cells were then treated with 0–104 pM of FGF21 or vehicle for 10 min.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_9244/pm38569244/pm38569244__page5_image1.jpg)
