mutant allele plant codon optimized cas9 potato intron  (Addgene inc)

Journal: Microbial Cell Factories

Article Title: Complete microbial synthesis of crocetin and crocins from glycerol in Escherichia coli

doi: 10.1186/s12934-023-02287-9

Figure Lengend Snippet: Engineered crocin biosynthetic pathway in Escherichia coli

Article Snippet: The E. coli codon-optimized versions of the plant-derived genes (csCCD2 from Crocus sativus , GjUGT1 from Gardenia jasminoides , GT1-316 from Populus fremontii x Populus angustifolia , NtUGT from Nicotiana tabacum , FaGT2 from Fragaria ananassa , StUGT from Solanum tuberosum , CaUGT3 from Catharanthus roseus , NsUGT from Nicotiana sylvestris , and SpUGT from Solanum pennellii ) were chemically synthesized (GenScript, Piscataway, NJ, USA) and individually subcloned into a plasmid pKK223-3 (Table ; Additional file : Table for a sequence of the synthetic genes).

Techniques:

Journal: Microbial Cell Factories

Article Title: Complete microbial synthesis of crocetin and crocins from glycerol in Escherichia coli

doi: 10.1186/s12934-023-02287-9

Figure Lengend Snippet: Microbial crocetin/crocin production

Article Snippet: The E. coli codon-optimized versions of the plant-derived genes (csCCD2 from Crocus sativus , GjUGT1 from Gardenia jasminoides , GT1-316 from Populus fremontii x Populus angustifolia , NtUGT from Nicotiana tabacum , FaGT2 from Fragaria ananassa , StUGT from Solanum tuberosum , CaUGT3 from Catharanthus roseus , NsUGT from Nicotiana sylvestris , and SpUGT from Solanum pennellii ) were chemically synthesized (GenScript, Piscataway, NJ, USA) and individually subcloned into a plasmid pKK223-3 (Table ; Additional file : Table for a sequence of the synthetic genes).

Techniques:

Journal: Microbial Cell Factories

Article Title: Complete microbial synthesis of crocetin and crocins from glycerol in Escherichia coli

doi: 10.1186/s12934-023-02287-9

Figure Lengend Snippet: E. coli strains and plasmids used in this study

Article Snippet: The E. coli codon-optimized versions of the plant-derived genes (csCCD2 from Crocus sativus , GjUGT1 from Gardenia jasminoides , GT1-316 from Populus fremontii x Populus angustifolia , NtUGT from Nicotiana tabacum , FaGT2 from Fragaria ananassa , StUGT from Solanum tuberosum , CaUGT3 from Catharanthus roseus , NsUGT from Nicotiana sylvestris , and SpUGT from Solanum pennellii ) were chemically synthesized (GenScript, Piscataway, NJ, USA) and individually subcloned into a plasmid pKK223-3 (Table ; Additional file : Table for a sequence of the synthetic genes).

Techniques: Plasmid Preparation, Sequencing, Cloning, Expressing

Journal: BMC Biotechnology

Article Title: Optimized production of a biologically active Clostridium perfringens glycosyl hydrolase phage endolysin PlyCP41 in plants using virus-based systemic expression

doi: 10.1186/s12896-019-0594-7

Figure Lengend Snippet: Comparison of the expression of PlyCp41p and PlyCp41pc in plants. Western blot of plant tissue collected 13 days post-infiltration. Neat (O.D. 600 nm = 2.7) and 1:10 (O. D at 600 nm = .27) represent dilutions of Agrobacterium cultures used to infiltrate plants with pGDPVXMCS: PlyCP41pc (CP41pc) or pGDPVXMCS:PlyCP41p mixed in a 1:10 dilution with Agrobacterium containing pGDp19. CP41p (A) designates a plant that was mechanically inoculated from a plant 22 days’ post-infiltration. This sample represents 7 days’ post-infection. rCP41 = 2 μg. M = Precision Plus Kaleidoscope protein standards

Article Snippet: A plant codon-optimized CP41 gene containing a C-terminal 6xHis-tag (PlyCP41pc) with a CAI index of 0.92 was synthesized by Genscript USA (Piscataway, NJ) and was cloned in the pJET1.2 vector [ ] (Additional file : Figure S1).

Techniques: Comparison, Expressing, Western Blot, Infection