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pla blocking solution  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology pla blocking solution
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Pla Blocking Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/Polylactic+acid/pmc12235512-148-3-12
    Average 93 stars, based on 7 article reviews
    pla blocking solution - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions"

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaf626

    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Figure Legend Snippet: Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Techniques Used: Fluorescence, Two Tailed Test, Immunoprecipitation, Irradiation, Membrane, Confocal Microscopy

    Related Articles

    other:

    Article Title: The protein composition of human adenovirus replication compartments
    Article Snippet: , Anti-HMGB1 mouse mAb W-18 , IF, WB, PLA , 1:200 , Santa Cruz Biotechnology.

    Article Title: The protein composition of human adenovirus replication compartments
    Article Snippet: Anti-53BP1 mouse mAb E-10 , PLA , 1:200 , Santa Cruz Biotechnology.

    Article Title: EZH2 inhibition sensitizes retinoic acid-driven senescence in synovial sarcoma
    Article Snippet: Antibody Dilution IF/PLA Catalog number Manufacturer Mouse anti PRAME 1:200 sc-137188 Santa Cruz Biotechnology, Dallas, TX, USA Mouse anti Tubulin β-III (2G10) 1:2000 ab78078 Abcam, Cambridge, UK Rabbit anti Retinoic acid Receptor alpha 1:200/1:250 PA1-810A Thermo Fisher Scientific, Waltham, MA, USA Rabbit anti EZH2 (D2C9) 1:200 5246S Cell Signaling Technology, Leiden, Netherlands

    Proximity Ligation Assay:

    Article Title: pH-Sensitive Naproxen Delivery via ZIF and Kaolin@ZIF Nanocarriers in 3D-Printed PLA–Gelatin Hydrogels
    Article Snippet: .. Polylactic Acid (PLA) filament (1.75 mm, matte black, eSUN, Shenzhen, China), pure gelatin powder (food quality) and glutaraldehyde solution (25% w / w in water; GA, Santa Cruz Biotechnology, Dallas, TX, USA), ethanol (EtOH, ≥70%, Sigma-Aldrich, St. Louis, MO, USA), Kaolin (Kao, powder, Santa Cruz Biotechnology, Dallas, TX, USA), zinc acetate dihydrate (Sigma-Aldrich, St. Louis, MO, USA), 2-methylimidazole (2-mIM, >99%, Isolab, Eschau, Germany) was used. .. Naproxen, as the model drug, was obtained from Santa Cruz Biotechnology.

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions
    Article Snippet: A proximity ligation assay (PLA) to detect endogenous interactions between XPC and BTG3 was performed using a Duo-link PLA kit (Sigma, # DUO92101) according to the manufacturer’s protocol. .. After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking. ..

    Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA
    Article Snippet: .. The primary antibody mixture contained PLA and IF antibodies (anti-NF-κB p65 (Santa Cruz Biotechnology; #sc-8008, diluted 1:100, ms and Bethyl Lab.; #A303-945A, diluted 1:1000, gt), anti-TFE3 (Sigma-Aldrich; #HPA023881, diluted 1:50, rb), anti-TFEB (Cell Signaling; #4240, diluted 1:25, rb), anti-GLIS2 (Thermo Fisher Scientific; #PA5-40314, diluted 1:25, rb), anti-ZBTB5 (Sigma, #HPA021521, diluted 1:150, rb) and anti-p50 (Santa Cruz Biotechnology; #sc-8414, diluted 1:100, ms), which were diluted in antibody diluent. ..

    Article Title: The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA.
    Article Snippet: .. The primary antibody mixture contained PLA and IF antibodies (anti-NF-κB p65 (Santa Cruz Biotechnology; #sc-8008, diluted 1:100, ms and Bethyl Lab.; #A303945A, diluted 1:1000, gt), anti-TFE3 (Sigma-Aldrich; #HPA023881, diluted 1:50, rb), anti-TFEB (Cell Signaling; #4240, diluted 1:25, rb), anti-GLIS2 (Thermo Fisher Scientific; #PA5-40314, diluted 1:25, rb), anti-ZBTB5 (Sigma, #HPA021521, diluted 1:150, rb) and antip50 (Santa Cruz Biotechnology; #sc-8414, diluted 1:100, ms), which were diluted in antibody diluent. ..

    Article Title: Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies.
    Article Snippet: Article Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies

    Incubation:

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions
    Article Snippet: A proximity ligation assay (PLA) to detect endogenous interactions between XPC and BTG3 was performed using a Duo-link PLA kit (Sigma, # DUO92101) according to the manufacturer’s protocol. .. After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking. ..

    Article Title: Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies.
    Article Snippet: Article Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies

    Blocking Assay:

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions
    Article Snippet: A proximity ligation assay (PLA) to detect endogenous interactions between XPC and BTG3 was performed using a Duo-link PLA kit (Sigma, # DUO92101) according to the manufacturer’s protocol. .. After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking. ..

    Gentle:

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions
    Article Snippet: A proximity ligation assay (PLA) to detect endogenous interactions between XPC and BTG3 was performed using a Duo-link PLA kit (Sigma, # DUO92101) according to the manufacturer’s protocol. .. After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking. ..

    Ligation:

    Article Title: Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies.
    Article Snippet: Article Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies

    Amplification:

    Article Title: Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies.
    Article Snippet: Article Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies

    Staining:

    Article Title: Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies.
    Article Snippet: Article Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies

    Confocal Microscopy:

    Article Title: Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies.
    Article Snippet: Article Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies



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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
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    Image Search Results


    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Journal: Nucleic Acids Research

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions

    doi: 10.1093/nar/gkaf626

    Figure Lengend Snippet: Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Article Snippet: After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking.

    Techniques: Fluorescence, Two Tailed Test, Immunoprecipitation, Irradiation, Membrane, Confocal Microscopy