Review



duolink blocking solution  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology duolink blocking solution
    ( A ) Effect of DNFB (5 μM) and knockdown of MTCK or CKB <t>on</t> <t>EGFR</t> phosphorylation. Lower panels, effect of siMTCK and siCKB on expression of MTCK and CKB, respectively. ( B ) Semi-quantification of EGFR phosphorylation levels. ( C ) Effect of ATP on suppressed EGFR phosphorylation by DNFB (5 μM). ( D ) Effect of oligomycin on EGFR phosphorylation. ( E ) Effect of CyCr on creatine phosphorylation. ( F ) Effect of CyCr on EGFR phosphorylation. ( G ) Co-immunoprecipitation using anti-CKB antibody or anti-EGFR antibody to examine binding of CKB and EGFR. ( H ) <t>Duolink</t> ® proximity ligation assay. Red, proximity signal of EGFR and CKB. Blue, DAPI. Scale bar, 10 μm. Error bars: standard deviation of three independent trials. Statistical significance was calculated using a two-tailed ordinary analysis of variance. Abbreviations: DNFB: dinitrofluorobenzene; EGFR: epithelial growth factor receptor; pEGFR: phosphorylated EGFR; EGF: epithelial growth factor; CKB: creatine kinase B; MTCK: mitochondrial creatine kinase; siC: short interfering RNA (siRNA) used as control; siMTCK: siRNA for MTCK; siCKB: siRNA for CKB; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; OM: oligomycin; CyCr: cyclocreatine; pCr: phosphocreatine; IP: immunoprecipitation; CB: Coomassie blue; WCL: whole cell lysate; DAPI: 4′,6-diamidino-2-phenylindole.
    Duolink Blocking Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2899 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/EGFR+Antibody/pmc10197964-262-30-45
    Average 96 stars, based on 2899 article reviews
    duolink blocking solution - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Role of creatine shuttle in colorectal cancer cells"

    Article Title: Role of creatine shuttle in colorectal cancer cells

    Journal: Oncotarget

    doi: 10.18632/oncotarget.28436

    ( A ) Effect of DNFB (5 μM) and knockdown of MTCK or CKB on EGFR phosphorylation. Lower panels, effect of siMTCK and siCKB on expression of MTCK and CKB, respectively. ( B ) Semi-quantification of EGFR phosphorylation levels. ( C ) Effect of ATP on suppressed EGFR phosphorylation by DNFB (5 μM). ( D ) Effect of oligomycin on EGFR phosphorylation. ( E ) Effect of CyCr on creatine phosphorylation. ( F ) Effect of CyCr on EGFR phosphorylation. ( G ) Co-immunoprecipitation using anti-CKB antibody or anti-EGFR antibody to examine binding of CKB and EGFR. ( H ) Duolink ® proximity ligation assay. Red, proximity signal of EGFR and CKB. Blue, DAPI. Scale bar, 10 μm. Error bars: standard deviation of three independent trials. Statistical significance was calculated using a two-tailed ordinary analysis of variance. Abbreviations: DNFB: dinitrofluorobenzene; EGFR: epithelial growth factor receptor; pEGFR: phosphorylated EGFR; EGF: epithelial growth factor; CKB: creatine kinase B; MTCK: mitochondrial creatine kinase; siC: short interfering RNA (siRNA) used as control; siMTCK: siRNA for MTCK; siCKB: siRNA for CKB; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; OM: oligomycin; CyCr: cyclocreatine; pCr: phosphocreatine; IP: immunoprecipitation; CB: Coomassie blue; WCL: whole cell lysate; DAPI: 4′,6-diamidino-2-phenylindole.
    Figure Legend Snippet: ( A ) Effect of DNFB (5 μM) and knockdown of MTCK or CKB on EGFR phosphorylation. Lower panels, effect of siMTCK and siCKB on expression of MTCK and CKB, respectively. ( B ) Semi-quantification of EGFR phosphorylation levels. ( C ) Effect of ATP on suppressed EGFR phosphorylation by DNFB (5 μM). ( D ) Effect of oligomycin on EGFR phosphorylation. ( E ) Effect of CyCr on creatine phosphorylation. ( F ) Effect of CyCr on EGFR phosphorylation. ( G ) Co-immunoprecipitation using anti-CKB antibody or anti-EGFR antibody to examine binding of CKB and EGFR. ( H ) Duolink ® proximity ligation assay. Red, proximity signal of EGFR and CKB. Blue, DAPI. Scale bar, 10 μm. Error bars: standard deviation of three independent trials. Statistical significance was calculated using a two-tailed ordinary analysis of variance. Abbreviations: DNFB: dinitrofluorobenzene; EGFR: epithelial growth factor receptor; pEGFR: phosphorylated EGFR; EGF: epithelial growth factor; CKB: creatine kinase B; MTCK: mitochondrial creatine kinase; siC: short interfering RNA (siRNA) used as control; siMTCK: siRNA for MTCK; siCKB: siRNA for CKB; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; OM: oligomycin; CyCr: cyclocreatine; pCr: phosphocreatine; IP: immunoprecipitation; CB: Coomassie blue; WCL: whole cell lysate; DAPI: 4′,6-diamidino-2-phenylindole.

    Techniques Used: Knockdown, Phospho-proteomics, Expressing, Immunoprecipitation, Binding Assay, Proximity Ligation Assay, Standard Deviation, Two Tailed Test, Small Interfering RNA, Control



    Similar Products

    pla blocking solution  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology pla blocking solution
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Pla Blocking Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/Polylactic+acid/pmc12235512-148-3-12
    Average 93 stars, based on 1 article reviews
    pla blocking solution - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    pla blocking solution  (Millipore)
    90
    Millipore pla blocking solution
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Pla Blocking Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/pla+blocking+solution/pm37965896-429-7-10
    Average 90 stars, based on 1 article reviews
    pla blocking solution - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    blocking solution included pla kit (duolink situ probe  (Merck & Co)
    90
    Merck & Co blocking solution included pla kit (duolink situ probe
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Blocking Solution Included Pla Kit (Duolink Situ Probe, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/duo92002/pm37604854-260-41-52
    Average 90 stars, based on 1 article reviews
    blocking solution included pla kit (duolink situ probe - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    duolink pla blocking solution  (Millipore)
    90
    Millipore duolink pla blocking solution
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Duolink Pla Blocking Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/duolink+pla/pm37480622-106-6-10
    Average 90 stars, based on 1 article reviews
    duolink pla blocking solution - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    blocking solution supplied pla kit  (Millipore)
    90
    Millipore blocking solution supplied pla kit
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Blocking Solution Supplied Pla Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/duolink+kit/pmc08921220-490-20-27
    Average 90 stars, based on 1 article reviews
    blocking solution supplied pla kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Journal: Nucleic Acids Research

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions

    doi: 10.1093/nar/gkaf626

    Figure Lengend Snippet: Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Article Snippet: After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking.

    Techniques: Fluorescence, Two Tailed Test, Immunoprecipitation, Irradiation, Membrane, Confocal Microscopy