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rabbit polyclonal anti megalin antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit polyclonal anti megalin antibody
    Basic demographic and clinicopathological features of involved subjects, and variables measured in the study.
    Rabbit Polyclonal Anti Megalin Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/megalin+Antibody/pmc08464971-85-26-31
    Average 93 stars, based on 142 article reviews
    rabbit polyclonal anti megalin antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Metallothioneins and Megalin Expression Profiling in Premalignant and Malignant Oral Squamous Epithelial Lesions"

    Article Title: Metallothioneins and Megalin Expression Profiling in Premalignant and Malignant Oral Squamous Epithelial Lesions

    Journal: Cancers

    doi: 10.3390/cancers13184530

    Basic demographic and clinicopathological features of involved subjects, and variables measured in the study.
    Figure Legend Snippet: Basic demographic and clinicopathological features of involved subjects, and variables measured in the study.

    Techniques Used: Biomarker Discovery

    Megalin expression in premalignant and malignant oral squamous epithelial lesions. Representative photomicrographs show immunohistochemical staining with the anti-megalin antibody on paraffin-embedded sections of tissue samples obtained from subjects with healthy oral mucosa ( A ), patients with oral lichen planus ( B ), oral leukoplakia ( C ), grade I OSCC ( D – F ), grade II OSCC ( G – I ), and grade III OSCC ( J – L ). Arrows on I and L indicate chromosomal megalin immunopositivity in mitotic cells. Magnifications: ( D , G , J ) × 100; ( A – C , E , F , H , K ) × 400; ( I , L ) × 1000.
    Figure Legend Snippet: Megalin expression in premalignant and malignant oral squamous epithelial lesions. Representative photomicrographs show immunohistochemical staining with the anti-megalin antibody on paraffin-embedded sections of tissue samples obtained from subjects with healthy oral mucosa ( A ), patients with oral lichen planus ( B ), oral leukoplakia ( C ), grade I OSCC ( D – F ), grade II OSCC ( G – I ), and grade III OSCC ( J – L ). Arrows on I and L indicate chromosomal megalin immunopositivity in mitotic cells. Magnifications: ( D , G , J ) × 100; ( A – C , E , F , H , K ) × 400; ( I , L ) × 1000.

    Techniques Used: Expressing, Immunohistochemical staining, Staining

    Quantified megalin staining intensities in premalignant and malignant oral squamous epithelial lesions. The measurements were made by ImageJ software and data are expressed in arbitrary units (AU) as the median of average gray values with range. HOM, healthy oral mucosa; OLP, oral lichen planus; OSCC, oral squamous cell carcinoma. * p < 0.000001 when each group is compared to the other.
    Figure Legend Snippet: Quantified megalin staining intensities in premalignant and malignant oral squamous epithelial lesions. The measurements were made by ImageJ software and data are expressed in arbitrary units (AU) as the median of average gray values with range. HOM, healthy oral mucosa; OLP, oral lichen planus; OSCC, oral squamous cell carcinoma. * p < 0.000001 when each group is compared to the other.

    Techniques Used: Staining, Software

    Co-expression and interaction of metallothionein I/II and megalin in oral squamous cell carcinoma. ( A ) Representative photomicrographs of double immunofluorescence staining with anti-MT I/II (red staining) and anti-megalin (green staining) antibodies on paraffin-embedded sections of the OSCC tissue. Blue marks DAPI staining of nuclei. Arrows indicate sites of membrane colocalization. Magnification: × 1000. ( B ) Representative photomicrographs obtained by proximity ligation assay (PLA) on paraffin-embedded sections of the OSCC tissue using anti-MT I/II and anti-megalin antibodies. Red fluorescent signals represent interaction sites. Blue marks DAPI staining of nuclei. Magnification: × 1000.
    Figure Legend Snippet: Co-expression and interaction of metallothionein I/II and megalin in oral squamous cell carcinoma. ( A ) Representative photomicrographs of double immunofluorescence staining with anti-MT I/II (red staining) and anti-megalin (green staining) antibodies on paraffin-embedded sections of the OSCC tissue. Blue marks DAPI staining of nuclei. Arrows indicate sites of membrane colocalization. Magnification: × 1000. ( B ) Representative photomicrographs obtained by proximity ligation assay (PLA) on paraffin-embedded sections of the OSCC tissue using anti-MT I/II and anti-megalin antibodies. Red fluorescent signals represent interaction sites. Blue marks DAPI staining of nuclei. Magnification: × 1000.

    Techniques Used: Expressing, Double Immunofluorescence Staining, Staining, Membrane, Proximity Ligation Assay

    Correlation of metallothionein I/II and megalin staining intensities. Pearson correlation analysis included data obtained from the overall study group. Correlation coefficient (r) = 0.8 ( p < 0.001).
    Figure Legend Snippet: Correlation of metallothionein I/II and megalin staining intensities. Pearson correlation analysis included data obtained from the overall study group. Correlation coefficient (r) = 0.8 ( p < 0.001).

    Techniques Used: Staining

    Impact of smoking habit on metallothionein I/II ( A ) and megalin ( B ) expression. The measurements were made by ImageJ software and data are expressed in arbitrary units (AU) as the median of average gray values with range. HOM, healthy oral mucosa; OLP, oral lichen planus; OSCC, oral squamous cell carcinoma.
    Figure Legend Snippet: Impact of smoking habit on metallothionein I/II ( A ) and megalin ( B ) expression. The measurements were made by ImageJ software and data are expressed in arbitrary units (AU) as the median of average gray values with range. HOM, healthy oral mucosa; OLP, oral lichen planus; OSCC, oral squamous cell carcinoma.

    Techniques Used: Expressing, Software

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    Article Snippet: Slides were mounted using VectaMount mounting medium (Vectorlabs) and scanned with Philips IntelliSite Ultra Fast Scanner.

    Incubation:

    Article Title: Heterogeneity in lysosomal dynamics and metabolic functions along the kidney proximal tubule.
    Article Snippet: .. Stainings were performed on 5 μm thick cryosections incubated overnight with the following antibodies: mouse anti-Megalin (H10) (1:100, Santa cruz Biotech, sc-515772), rabbit anti-Rab5 (1:50, Cell signaling, #3547), AR TI CL E IN P RE SS rabbit anti-Rab7 (1:50, Abcam, ab137029), goat anti-Cathepsin L (1:100, R&D Systems, AF1515), rabbit anti-OAT1 (1:100, Alpha diagnostic international, OAT11-A). .. LAL staining was performed in paraffin embedded tissues, following a citrate pH 6 buffer antigen retrieval step at high temperature and pressure, using mouse anti-LAL (1:100, Santa cruz Biotech, sc-58374).

    Article Title: Lipocalin‐2 Restores Soluble Guanylyl Cyclase‐Dependent Dilation of the Afferent Arteriole After Renal Transplantation or Ex Vivo Hypoxia/Reoxygenation in Mice
    Article Snippet: The membranes were blocked for 1 h at room temperature with 5% skim milk (Applichem, Darmstadt, Germany) and 1% bovine serum albumin (SERVA, Heidelberg, Germany), dissolved in TBST (50 mM TRIZMA base, 150 mM NaCl, pH 7.5 adjusted with HCl, 0.1% Tween‐20). .. The membranes were then incubated overnight at 4°C with the following primary antibodies: anti‐Lrp2 (sc‐515772, Santa Cruz Biotechnology, CA, USA), anti‐Akt (#9272, Cell Signaling Technologies). ..

    Article Title: Single‐Chain Anti‐IL‐1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4°C: anti‐His (MA5‐33032, Invitrogen; 1:2000 dilution) and anti‐megalin (sc‐515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.

    Article Title: Lipocalin-2 Restores Soluble Guanylyl Cyclase-Dependent Dilation of the Afferent Arteriole After Renal Transplantation or Ex Vivo Hypoxia/Reoxygenation in Mice.
    Article Snippet: The membranes were blocked for 1 h at room temperature with 5% skim milk (Applichem, Darmstadt, Germany) and 1% bovine serum albumin (SERVA, Heidelberg, Germany), dissolved in TBST (50 mM TRIZMA base, 150 mM NaCl, pH 7.5 adjusted with HCl, 0.1% Tween- 20). .. The membranes were then incubated overnight at 4°C with the following primary antibodies: anti- Lrp2 (sc- 515772, Santa Cruz Biotechnology, CA, USA), antiAkt (#9272, Cell Signaling Technologies). ..

    Article Title: Single-Chain Anti-IL-1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease.
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4◦C: anti-His (MA5-33032, Invitrogen; 1:2000 dilution) and anti-megalin (sc-515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.

    Biomarker Discovery:

    Article Title:
    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used anti-Wilms’ tumor-1 (WT1; ab89901, Abcam plc. .. / sc-7385, Santa Cruz Biotechnology Inc.); anti-cytokeratin 8 (CK8 (TROMA-I); DSHB); anti-human nuclei (HuNu; MAB4383, Merck Millipore); anti-Ku80 (2180S, Cell Signaling Technology, Inc.); anti-lotus tetragonolobus 3 nature portfolio | reporting sum m ary M arch 2021 lectin (LTL; B-1325, Vector Laboratories, Inc.); anti-E-cadherin-1 (ECAD; 610181, BD Biosciences); anti-nephrin (GP-N2, Vector Laboratories, Inc.); anti-podocalyxin (PODXL; AF1658, R&D Systems, Inc.); anti-Jagged 1 (JAG1; sc-390177, Santa Cruz Biotechnology Inc.); anti-cadherin 6 (CDH6; AF1658, R&D Systems, Inc.); anti-aquaporin 1 (AQP1; ab9566, Abcam plc.); anti-megalin (sc-515772, Santa Cruz Biotechnology Inc.); anti-Dolichos biflorus agglutinin (DBA; B-1035, Vector Laboratories, Inc.); anti-sodium-chloride cotransporter (NCC; AB3553, Merck Millipore); anti-integrin alpha 8(ITGA8; BAF4076, R&D Systems, Inc.); anti-platlet-derived growth factor receptor alpha (PDGFRA; 323506, BioLegend, Inc.) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer. .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used anti-Wilms’ tumor-1 (WT1; ab89901, Abcam plc.

    Blocking Assay:

    Article Title: Single‐Chain Anti‐IL‐1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4°C: anti‐His (MA5‐33032, Invitrogen; 1:2000 dilution) and anti‐megalin (sc‐515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.

    Article Title: Single-Chain Anti-IL-1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease.
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4◦C: anti-His (MA5-33032, Invitrogen; 1:2000 dilution) and anti-megalin (sc-515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.

    Staining:

    Article Title: Single‐Chain Anti‐IL‐1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4°C: anti‐His (MA5‐33032, Invitrogen; 1:2000 dilution) and anti‐megalin (sc‐515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.

    Article Title: Single-Chain Anti-IL-1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease.
    Article Snippet: .. After blocking for 1 h with 5% BSA, the samples were incubated with the following primary antibodies overnight at 4◦C: anti-His (MA5-33032, Invitrogen; 1:2000 dilution) and anti-megalin (sc-515772, Santa Cruz, USA; 1:100 dilution); the samples were then incubated with the corresponding secondary antibodies at R/T for 1 h. The nuclei were stained with DAPI. .. An Olympus inverted fluorescence microscope was used to acquire images.



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    Article Snippet: After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking.

    Techniques: Fluorescence, Two Tailed Test, Immunoprecipitation, Irradiation, Membrane, Confocal Microscopy