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goat serum vector laboratories  (Vector Laboratories)


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    Structured Review

    Vector Laboratories goat serum vector laboratories
    Goat Serum Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 6336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/Normal+Goat+Serum+Blocking+Solution/pm40499536-278-40-42
    Average 96 stars, based on 6336 article reviews
    goat serum vector laboratories - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Plasmid Preparation:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Binding Assay:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Incubation:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Staining:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Blocking Assay:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Saline:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Immunohistochemistry:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Agarose Gel Electrophoresis:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Fractionation:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Size-exclusion Chromatography:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Ethanol Precipitation:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Purification:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Extraction:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi

    Chromatography:

    Article Title: Systematic discovery of immunomodulatory plant-derived nanoparticles reveals RNA-mediated macrophage reprogramming
    Article Snippet: cam) for 10 min, followed by cooling at room temperature for 2 h. Endogenous peroxidase activity was quenched using 3% hydrogen peroxide, and nonspecific binding was blocked with 5% normal goat serum (Vector Laboratories, S-1000) in PBS. Sections were incubated overnight at 4 °C with primary antibodies diluted in 5% normal goat serum (100 μL per slide). After washing, appropriate secondary antibodi



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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Journal: Nucleic Acids Research

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions

    doi: 10.1093/nar/gkaf626

    Figure Lengend Snippet: Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Article Snippet: After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking.

    Techniques: Fluorescence, Two Tailed Test, Immunoprecipitation, Irradiation, Membrane, Confocal Microscopy