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pla blocking solution  (Millipore)


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    Millipore pla blocking solution
    Pla Blocking Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/pla+blocking+solution/pm37965896-429-7-10
    Average 90 stars, based on 1 article reviews
    pla blocking solution - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Proximity Ligation Assay:

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..

    Article Title: Protein Kinase D3 (PKD3) Requires Hsp90 for Stability and Promotion of Prostate Cancer Cell Migration
    Article Snippet: .. Wells were blocked with PLA Blocking solution (Sigma Aldrich, St. Louis, MO, USA) for 1 h, then primary antibodies diluted in PLA antibody diluent solution (Sigma Aldrich, St. Louis, MO, USA) were added and incubated overnight at 4 °C. .. Rabbit polyclonal anti-Akt1 antibody was from Sigma–Aldrich, mouse monoclonal anti-Hsp90 from Institute of Immunology Ltd. (Tokyo, Japan), and rabbit polyclonal anti-PKD2 and rabbit polyclonal anti-PKD3 antibodies from Atlas Antibodies (Bromma, Sweden).

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma‐Aldrich) overnight at 4°C then washed with PBS. .. PLA probes (antimouse minus DUO92004 and antirabbit plus DUO92002, Sigma‐Aldrich) were incubated together in PLA blocking solution for 20 min then added on the coverslips for 1 h at 37°C then washed two times with buffer A (150 mM NaCl, 10 mM Tris, 0.5% Tween).

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma-Aldrich) overnight at 4°C then washed with PBS. .. PLA probes (antimouse minus DUO92004 and antirabbit plus DUO92002, Sigma-Aldrich) were incubated together in PLA blocking solution for 20 min then added on the coverslips for 1 h at 37°C then washed two times with buffer A (150 mM NaCl, 10 mM Tris, 0.5% Tween).

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..

    Blocking Assay:

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..

    Article Title: Protein Kinase D3 (PKD3) Requires Hsp90 for Stability and Promotion of Prostate Cancer Cell Migration
    Article Snippet: .. Wells were blocked with PLA Blocking solution (Sigma Aldrich, St. Louis, MO, USA) for 1 h, then primary antibodies diluted in PLA antibody diluent solution (Sigma Aldrich, St. Louis, MO, USA) were added and incubated overnight at 4 °C. .. Rabbit polyclonal anti-Akt1 antibody was from Sigma–Aldrich, mouse monoclonal anti-Hsp90 from Institute of Immunology Ltd. (Tokyo, Japan), and rabbit polyclonal anti-PKD2 and rabbit polyclonal anti-PKD3 antibodies from Atlas Antibodies (Bromma, Sweden).

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma‐Aldrich) overnight at 4°C then washed with PBS. .. PLA probes (antimouse minus DUO92004 and antirabbit plus DUO92002, Sigma‐Aldrich) were incubated together in PLA blocking solution for 20 min then added on the coverslips for 1 h at 37°C then washed two times with buffer A (150 mM NaCl, 10 mM Tris, 0.5% Tween).

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma-Aldrich) overnight at 4°C then washed with PBS. .. PLA probes (antimouse minus DUO92004 and antirabbit plus DUO92002, Sigma-Aldrich) were incubated together in PLA blocking solution for 20 min then added on the coverslips for 1 h at 37°C then washed two times with buffer A (150 mM NaCl, 10 mM Tris, 0.5% Tween).

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..

    Incubation:

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..

    Article Title: Protein Kinase D3 (PKD3) Requires Hsp90 for Stability and Promotion of Prostate Cancer Cell Migration
    Article Snippet: .. Wells were blocked with PLA Blocking solution (Sigma Aldrich, St. Louis, MO, USA) for 1 h, then primary antibodies diluted in PLA antibody diluent solution (Sigma Aldrich, St. Louis, MO, USA) were added and incubated overnight at 4 °C. .. Rabbit polyclonal anti-Akt1 antibody was from Sigma–Aldrich, mouse monoclonal anti-Hsp90 from Institute of Immunology Ltd. (Tokyo, Japan), and rabbit polyclonal anti-PKD2 and rabbit polyclonal anti-PKD3 antibodies from Atlas Antibodies (Bromma, Sweden).

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma‐Aldrich) overnight at 4°C then washed with PBS. .. PLA probes (antimouse minus DUO92004 and antirabbit plus DUO92002, Sigma‐Aldrich) were incubated together in PLA blocking solution for 20 min then added on the coverslips for 1 h at 37°C then washed two times with buffer A (150 mM NaCl, 10 mM Tris, 0.5% Tween).

    Article Title: The nucleolar protein GNL3 prevents resection of stalled replication forks.
    Article Snippet: .. Coverslips were incubated with primary antibodies in PLA blocking solution (Sigma-Aldrich) overnight at 4°C then washed with PBS. .. PLA probes (antimouse minus DUO92004 and antirabbit plus DUO92002, Sigma-Aldrich) were incubated together in PLA blocking solution for 20 min then added on the coverslips for 1 h at 37°C then washed two times with buffer A (150 mM NaCl, 10 mM Tris, 0.5% Tween).

    Article Title: Transgenic Mice Expressing Human α-Synuclein in Noradrenergic Neurons Develop Locus Ceruleus Pathology and Nonmotor Features of Parkinson's Disease
    Article Snippet: Slides were then incubated for 1 h with secondary antibodies conjugated to Life Technologies Alexa Fluor 488 (Thermo Fisher Scientific) and washed again with TBS-T. For immunohistochemistry, samples were then coverslipped with FluorSave (Calbiochem). .. For PLA, samples were covered in PLA blocking solution (Sigma-Aldrich) for 1 h at 37°C, and then incubated overnight with PLA conjugates (a-syn211; catalog #ab80627, Abcam). ..



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    Santa Cruz Biotechnology pla blocking solution
    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Pla Blocking Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pla blocking solution  (Millipore)
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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
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    blocking solution included pla kit (duolink situ probe  (Merck & Co)
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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Blocking Solution Included Pla Kit (Duolink Situ Probe, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    duolink pla blocking solution  (Millipore)
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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
    Duolink Pla Blocking Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    duolink pla blocking solution - by Bioz Stars, 2026-09
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    blocking solution supplied pla kit  (Millipore)
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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
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    Image Search Results


    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Journal: Nucleic Acids Research

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions

    doi: 10.1093/nar/gkaf626

    Figure Lengend Snippet: Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Article Snippet: After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking.

    Techniques: Fluorescence, Two Tailed Test, Immunoprecipitation, Irradiation, Membrane, Confocal Microscopy