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blocking solution included pla kit (duolink situ probe  (Merck & Co)

 
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    Merck & Co blocking solution included pla kit (duolink situ probe
    Blocking Solution Included Pla Kit (Duolink Situ Probe, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pla+blocking+solution/duo92002/pm37604854-260-41-52
    Average 90 stars, based on 1 article reviews
    blocking solution included pla kit (duolink situ probe - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Proximity Ligation Assay:

    Article Title: Early tolerance and late persistence as alternative drug responses in cancer
    Article Snippet: .. The next day, cells were fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.5% Triton-X and then proximity ligation assay was performed following customer instructions (PLA Duolink Merck). .. Briefly, cells were incubated with the primary antibodies against HNF4A (K9218; Invitrogen) and PINK1 (NovusBio) for 1 h and 30’ at room temperature.

    Article Title: Kaempferol enhances ER-mitochondria coupling and protects motor neurons from mitochondrial dysfunction and ER stress in C9ORF72 -ALS
    Article Snippet: .. Slides were kept in a humid chamber and blocking solution was added (Duolink PLA probe kit, DUO92008, Merck) for 1 h at 37 °C. .. Subsequently, slides were incubated with the following primary antibodies (1:200, rabbit anti-IP3R, ABCAM, ab5804; 1:100, mouse anti-GRP75, ABCAM, ab53098; 1:100, rabbit anti-GRP75, ABCAM, ab227215; 1:200 rabbit anti-ATP5J, Thermo Fisher Scientific, PA5-29202) in a humidity chamber for 2 nights at 4 °C.

    Article Title: Early tolerance and late persistence as alternative drug responses in cancer.
    Article Snippet: .. The next day, cells were fixed with 4% paraformaldehyde for 10min, permeabilized with 0.5% Triton-X and then proximity ligation assay was performed following customer instructions (PLA Duolink Merck). .. Briefly, cells were incubated with the primary antibodies against HNF4A (K9218; Invitrogen) and PINK1 (NovusBio) for 1 h and 30’ at room temperature.

    Article Title: Kaempferol enhances ER-mitochondria coupling and protects motor neurons from mitochondrial dysfunction and ER stress in C9ORF72-ALS.
    Article Snippet: .. Slides were kept in a humid chamber and blocking solution was added (Duolink PLA probe kit, DUO92008, Merck) for 1 h at 37 °C. .. Subsequently, slides were incubated with the following primary antibodies (1:200, rabbit anti-IP3R, ABCAM, ab5804; 1:100, mouse anti-GRP75, ABCAM, ab53098; 1:100, rabbit anti-GRP75, ABCAM, ab227215; 1:200 rabbit anti-ATP5J, Thermo Fisher Scientific, PA5-29202) in a humidity chamber for 2 nights at 4 °C.

    Article Title: Phosphorylation-dependent WRN-RPA interaction promotes recovery of stalled forks at secondary DNA structure.
    Article Snippet: .. The in situ proximity ligation assay (PLA) in combination with immunofluorescence microscopy was performed using the Duolink Detection (Merck) or the NaveniFlex (Navinci diagnostics) Kit with anti-Mouse PLUS and anti-Rabbit MINUS PLA Probes, according to the manufacturer’s instructions. .. To detect proteins, we used rabbit anti-WRN (Abcam), rabbit anti-Flag (Sigma-Aldrich), mouse anti-RPA34-20 (Millipore), rabbit anti-RAD51 (Santa Cruz) and mouse anti-IdU antibody (Becton Dickinson) antibodies.

    Blocking Assay:

    Article Title: Kaempferol enhances ER-mitochondria coupling and protects motor neurons from mitochondrial dysfunction and ER stress in C9ORF72 -ALS
    Article Snippet: .. Slides were kept in a humid chamber and blocking solution was added (Duolink PLA probe kit, DUO92008, Merck) for 1 h at 37 °C. .. Subsequently, slides were incubated with the following primary antibodies (1:200, rabbit anti-IP3R, ABCAM, ab5804; 1:100, mouse anti-GRP75, ABCAM, ab53098; 1:100, rabbit anti-GRP75, ABCAM, ab227215; 1:200 rabbit anti-ATP5J, Thermo Fisher Scientific, PA5-29202) in a humidity chamber for 2 nights at 4 °C.

    Article Title: Kaempferol enhances ER-mitochondria coupling and protects motor neurons from mitochondrial dysfunction and ER stress in C9ORF72-ALS.
    Article Snippet: .. Slides were kept in a humid chamber and blocking solution was added (Duolink PLA probe kit, DUO92008, Merck) for 1 h at 37 °C. .. Subsequently, slides were incubated with the following primary antibodies (1:200, rabbit anti-IP3R, ABCAM, ab5804; 1:100, mouse anti-GRP75, ABCAM, ab53098; 1:100, rabbit anti-GRP75, ABCAM, ab227215; 1:200 rabbit anti-ATP5J, Thermo Fisher Scientific, PA5-29202) in a humidity chamber for 2 nights at 4 °C.

    Construct:

    Article Title: The MYCN/Aurora-A complex is a cyclin activating kinase for CDK12
    Article Snippet: Cells were treated with inhibitors as indicated and if cells were differentiated according to cell cycle phases, conditions were pulsed with 10 μM EdU (Jena Bioscience). .. For SH-EP MYCN wt /MYCN 3A 750 cells were seeded in a 384 well format and constructs were induced with 1 μg/ml doxycycline (Sigma-Aldrich) for 24 h. Cells were fixed/permeabilized with 100% methanol (Merck) on ice for 20 min. PLA was performed according to manufacturer’s instructions (Duolink In Situ Proximity Ligation Assay, Merck). ..

    In Situ:

    Article Title: The MYCN/Aurora-A complex is a cyclin activating kinase for CDK12
    Article Snippet: Cells were treated with inhibitors as indicated and if cells were differentiated according to cell cycle phases, conditions were pulsed with 10 μM EdU (Jena Bioscience). .. For SH-EP MYCN wt /MYCN 3A 750 cells were seeded in a 384 well format and constructs were induced with 1 μg/ml doxycycline (Sigma-Aldrich) for 24 h. Cells were fixed/permeabilized with 100% methanol (Merck) on ice for 20 min. PLA was performed according to manufacturer’s instructions (Duolink In Situ Proximity Ligation Assay, Merck). ..

    Article Title: Endothelial STING-JAK1 interaction promotes tumor vasculature normalization and antitumor immunity
    Article Snippet: Briefly, sections were washed and incubated for 1 hour at 37°C with Duolink PLA probes MINUS and PLUS (Merck, Cat DUO92002 and DUO92004). .. A Duolink in situ detection kit (Merck, Cat DUO92008) was used to ligate and amplify the signal, and nuclei were stained with DAPI (Merck, Cat DUO82040). .. Finally, images were captured using confocal scanning fluorescence microscopy (ZEISS, LSM880), with red spots indicating palmitoylated STING signal.

    Article Title: Phosphorylation-dependent WRN-RPA interaction promotes recovery of stalled forks at secondary DNA structure.
    Article Snippet: .. The in situ proximity ligation assay (PLA) in combination with immunofluorescence microscopy was performed using the Duolink Detection (Merck) or the NaveniFlex (Navinci diagnostics) Kit with anti-Mouse PLUS and anti-Rabbit MINUS PLA Probes, according to the manufacturer’s instructions. .. To detect proteins, we used rabbit anti-WRN (Abcam), rabbit anti-Flag (Sigma-Aldrich), mouse anti-RPA34-20 (Millipore), rabbit anti-RAD51 (Santa Cruz) and mouse anti-IdU antibody (Becton Dickinson) antibodies.

    Staining:

    Article Title: Endothelial STING-JAK1 interaction promotes tumor vasculature normalization and antitumor immunity
    Article Snippet: Briefly, sections were washed and incubated for 1 hour at 37°C with Duolink PLA probes MINUS and PLUS (Merck, Cat DUO92002 and DUO92004). .. A Duolink in situ detection kit (Merck, Cat DUO92008) was used to ligate and amplify the signal, and nuclei were stained with DAPI (Merck, Cat DUO82040). .. Finally, images were captured using confocal scanning fluorescence microscopy (ZEISS, LSM880), with red spots indicating palmitoylated STING signal.

    Immunofluorescence:

    Article Title: Phosphorylation-dependent WRN-RPA interaction promotes recovery of stalled forks at secondary DNA structure.
    Article Snippet: .. The in situ proximity ligation assay (PLA) in combination with immunofluorescence microscopy was performed using the Duolink Detection (Merck) or the NaveniFlex (Navinci diagnostics) Kit with anti-Mouse PLUS and anti-Rabbit MINUS PLA Probes, according to the manufacturer’s instructions. .. To detect proteins, we used rabbit anti-WRN (Abcam), rabbit anti-Flag (Sigma-Aldrich), mouse anti-RPA34-20 (Millipore), rabbit anti-RAD51 (Santa Cruz) and mouse anti-IdU antibody (Becton Dickinson) antibodies.

    Microscopy:

    Article Title: Phosphorylation-dependent WRN-RPA interaction promotes recovery of stalled forks at secondary DNA structure.
    Article Snippet: .. The in situ proximity ligation assay (PLA) in combination with immunofluorescence microscopy was performed using the Duolink Detection (Merck) or the NaveniFlex (Navinci diagnostics) Kit with anti-Mouse PLUS and anti-Rabbit MINUS PLA Probes, according to the manufacturer’s instructions. .. To detect proteins, we used rabbit anti-WRN (Abcam), rabbit anti-Flag (Sigma-Aldrich), mouse anti-RPA34-20 (Millipore), rabbit anti-RAD51 (Santa Cruz) and mouse anti-IdU antibody (Becton Dickinson) antibodies.

    other:

    Article Title: Endothelial STING-JAK1 interaction promotes tumor vasculature normalization and antitumor immunity
    Article Snippet: Briefly, sections were washed and incubated for 1 hour at 37°C with Duolink PLA probes MINUS and PLUS (Merck, Cat DUO92002 and DUO92004).



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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
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    blocking solution included pla kit (duolink situ probe  (Merck & Co)
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    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. <t>PLA</t> was conducted <t>with</t> <t>HaCaT</t> cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).
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    Image Search Results


    Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Journal: Nucleic Acids Research

    Article Title: BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions

    doi: 10.1093/nar/gkaf626

    Figure Lengend Snippet: Loss of BTG3 leads to reduced mobility of XPC at damage sites. ( A – C ) Colocalization of BTG3 and XPC at damage sites after UV. PLA was conducted with HaCaT cells using anti-BTG3 and anti-XPC (A). Mean fluorescence intensity and PLA foci number were quantified and shown in panels (B) and (C), respectively, with n ≧ 100. Data were analyzed using unpaired two-tailed t -test and presented as mean ± SEM Scale bar, 10 μm. ( D ) Co-immunoprecipitation of endogenous BTG3 and XPC from HaCaT cells. ( E, F ) Retention of XPC at localized UV damage in BTG3 KO cells. Localized UVC-irradiation (100 J/m 2 ) was performed with 5 μM isopore membrane filter and XPC loading at localized UV damage sites was assessed using confocal microscopy (E). Scale bar, 10 μm. Percent cells with XPC-positive foci were counted and presented as mean ± SEM in panel (F). Data were analyzed using unpaired two-tailed t -test. n ≧ 50. ( G, H ) Mobility of XPC was reduced in BTG3 KO cells. The mobility of XPC-GFP in mock-treated or global UV-irradiated (20 J/m 2 ) parental and BTG3 KO HEK293T cells was assessed using FRAP. Fluorescence recovery was monitored over 120 s and normalized to prebleach intensity ( n = 36 from 3 independent experiments) (G). Data were analyzed using unpaired two-tailed t -tests and shown as mean ± SEM in panel (H).

    Article Snippet: After incubation with PLA blocking solution, HaCaT cells were incubated with anti-XPC (Santa Cruz, 1:200 dilution) and anti-BTG3 (Lab raised, 1:100 dilution) antibodies overnight at 4°C with gentle rocking.

    Techniques: Fluorescence, Two Tailed Test, Immunoprecipitation, Irradiation, Membrane, Confocal Microscopy