pkg pka in buffer  (Bio-Rad)

Journal: Cell chemical biology

Article Title: Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase

doi: 10.1016/j.chembiol.2017.09.011

Figure Lengend Snippet: Key Resources Table

Article Snippet: Assessment of C W196R/Y204A Substrate Specificity VASP (80 ng) was incubated with or without 500 ng PKG/PKA in buffer (50 mM Tris + 100 NaCl + 10 mM MgCl 2 + 1 mM DTT + 1 mM EDTA + 1 mM ATP) with a final volume of 100 μL at room temperature for 30 min. Western blots of all samples, 10 μL per well, were performed on 4–20% SDS Tris-HCl gels (Biorad) and transferred to nitrocellulose membranes.

Techniques: Recombinant, Transfection, Isolation, Cell Culture, Protease Inhibitor, Bradford Protein Assay, Electron Microscopy, Lysis, Western Blot, Stripping Membranes, Mutagenesis, Amplification, Sequencing, Plasmid Preparation, Modification, Software