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BioComp Systems Inc gradient master
Gradient Master, supplied by BioComp Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionator/piston+gradient+fractionator/pmc03257569-237-5-7
Average 90 stars, based on 1 article reviews
gradient master - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Fatty Acid Remodeling of GPI-anchored Proteins Is Required for Their Raft Association
Article Snippet: After ultracentrifugation at 35,000 rpm (SW41 rotor) at 4°C for 16–18 h, fractions of 1 ml were collected from the top using Piston Gradient Fractionator (BioComp Systems).

Article Title: Cryo-EM structure of the chain-elongating E3 ubiquitin ligase UBR5.
Article Snippet: Gradients were made by mixing the two solutions using the Gradient Master (Biocomp systems) to create a continuous sucrose gradient.

Article Title: Implications of lipid moiety in oligomerization and immunoreactivities of GPI-anchored proteins
Article Snippet: One milliliter of lysate was mixed with 1 ml of 80% sucrose in MBS-E, transferred to a centrifuge tube for SW41-Ti, overlaid with 7 ml of 30% and 2 ml of 5% sucrose in MBS-E, ultracentrifuged at 38,000 rpm for 16–18 h at 4°C, and fractionated from the top using Piston Gradient Fractionator (BioComp Systems) with each fraction of 1 ml (total 11 fractions).

Article Title: Fatty Acid Remodeling of GPI-anchored Proteins Is Required for Their Raft Association
Article Snippet: One milliliter of lysate was mixed with 1 ml of 80% (wt/vol) sucrose in MBS-E, transferred to a centrifuge tube for SW41, overlaid with 7 ml of 30% and 2 ml of 5% sucrose in MBS-E, ultracentrifuged at 38,000 rpm for 16–18 h at 4°C, and fractionated from the top using Piston Gradient Fractionator (BioComp Systems) with each fraction of 1 ml (total 11 fractions).

Article Title: Fatty Acid Remodeling of GPI-anchored Proteins Is Required for Their Raft Association
Article Snippet: All supernatants were combined (∼18 ml in total), and 1.5 ml each of the combined supernatant was placed on each of 10 ml of a continuous sucrose gradient (20–50%, wt/vol) in 20 mM HEPES-NaOH, pH 7.4, prepared in 12 tubes by Gradient Master (BioComp Systems, Minneapolis, MN).

Generated:

Article Title: Eisosome proteins assemble into a membrane scaffold
Article Snippet: .. Gradients were generated using a gradient master (BioComp Systems, Inc.). .. The gradients were spun in an ultracentrifuge (WX Ultra Series; Thermo Fisher Scientific) equipped with a TH-641 rotor at 40,000 rpm at 4°C for 3.5 h. 1-ml fractions were manually pipetted, and proteins were precipitated using TCA and analyzed by SDS-PAGE and Coomassie blue staining.



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